NUP67

NUP67 (TG_306560) is a component of the Toxoplasma gondii nuclear pore complex, identified as a TgNup302-interacting protein by affinity tag purification and mass spectrometry, with nuclear pore localization of the complex confirmed by structured illumination microscopy (PMID:28138739). Beyond this association, no functional mechanism has been characterized for NUP67 in the available corpus.

1. 2017 Low

   Whether NUP67 is a constituent of the Toxoplasma nuclear pore was unknown; co-purification with TgNup302 placed it within the NPC interaction network and established it as a candidate nucleoporin.

   Evidence Affinity tag purification with mass spectrometry, reverse immunoprecipitation, and structured illumination microscopy in T. gondii

   PMID:28138739

   Open questions at the time

   • NUP67 was identified only within a bulk interaction screen; no functional experiments were performed on NUP67 itself
   • Direct NPC localization of NUP67 (as opposed to the complex) was not independently demonstrated
   • The specific structural or transport role of NUP67 within the NPC is undefined