{"gene":"TG_306560","gene_symbol":"NUP67","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2017,"finding":"The Toxoplasma gondii nuclear pore complex (NPC), which includes NUP67 (TG_306560) as one of the TgNup302-interacting proteins identified by affinity tag purification and mass spectrometry, localizes to the nuclear pore as confirmed by structured illumination microscopy.","method":"Affinity tag purification combined with mass spectrometry; reverse immunoprecipitation; structured illumination microscopy","journal":"Cellular and molecular life sciences : CMLS","confidence":"Low","confidence_rationale":"Tier 3 / Weak — NUP67 was identified as a component of the TgNup302 interaction network in a single mass spectrometry screen; no functional experiments were performed specifically on NUP67 itself.","pmids":["28138739"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"NUP67 (TG_306560) has been identified as a component of the Toxoplasma gondii nuclear pore complex through co-purification with TgNup302 and mass spectrometry, with NPC localization supported by structured illumination microscopy, but no functional mechanism has been established for NUP67 specifically."},"narrative":{"mechanistic_narrative":"NUP67 (TG_306560) is a component of the Toxoplasma gondii nuclear pore complex, identified as a TgNup302-interacting protein by affinity tag purification and mass spectrometry, with nuclear pore localization of the complex confirmed by structured illumination microscopy [PMID:28138739]. Beyond this association, no functional mechanism has been characterized for NUP67 in the available corpus.","teleology":[{"year":2017,"claim":"Whether NUP67 is a constituent of the Toxoplasma nuclear pore was unknown; co-purification with TgNup302 placed it within the NPC interaction network and established it as a candidate nucleoporin.","evidence":"Affinity tag purification with mass spectrometry, reverse immunoprecipitation, and structured illumination microscopy in T. gondii","pmids":["28138739"],"confidence":"Low","gaps":["NUP67 was identified only within a bulk interaction screen; no functional experiments were performed on NUP67 itself","Direct NPC localization of NUP67 (as opposed to the complex) was not independently demonstrated","The specific structural or transport role of NUP67 within the NPC is undefined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii nuclear pore complex (TgNup302 interaction network)"],"partners":["TGNUP302"],"other_free_text":["Identified as a TgNup302-interacting protein by affinity purification-mass spectrometry; no NUP67-specific functional characterization available."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"28138739","id":"PMC_28138739","title":"Characterization of a nuclear pore protein sheds light on the roles and composition of the Toxoplasma gondii nuclear pore complex.","date":"2017","source":"Cellular and molecular life sciences : CMLS","url":"https://pubmed.ncbi.nlm.nih.gov/28138739","citation_count":13,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2996,"output_tokens":1027,"usd":0.012197,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5956,"output_tokens":2234,"usd":0.042815,"stage2_stop_reason":"end_turn"},"total_usd":0.088157,"stage1_batch_id":"msgbatch_01S6GjoYQm8LTPUsdhASdqag","stage2_batch_id":"msgbatch_01VX63RTMdBJqsG6gQMqoCjz","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"The Toxoplasma gondii nuclear pore complex (NPC), which includes NUP67 (TG_306560) as one of the TgNup302-interacting proteins identified by affinity tag purification and mass spectrometry, localizes to the nuclear pore as confirmed by structured illumination microscopy.\",\n      \"method\": \"Affinity tag purification combined with mass spectrometry; reverse immunoprecipitation; structured illumination microscopy\",\n      \"journal\": \"Cellular and molecular life sciences : CMLS\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — NUP67 was identified as a component of the TgNup302 interaction network in a single mass spectrometry screen; no functional experiments were performed specifically on NUP67 itself.\",\n      \"pmids\": [\"28138739\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"NUP67 (TG_306560) has been identified as a component of the Toxoplasma gondii nuclear pore complex through co-purification with TgNup302 and mass spectrometry, with NPC localization supported by structured illumination microscopy, but no functional mechanism has been established for NUP67 specifically.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"NUP67 (TG_306560) is a component of the Toxoplasma gondii nuclear pore complex, identified as a TgNup302-interacting protein by affinity tag purification and mass spectrometry, with nuclear pore localization of the complex confirmed by structured illumination microscopy [#0]. Beyond this association, no functional mechanism has been characterized for NUP67 in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"Whether NUP67 is a constituent of the Toxoplasma nuclear pore was unknown; co-purification with TgNup302 placed it within the NPC interaction network and established it as a candidate nucleoporin.\",\n      \"evidence\": \"Affinity tag purification with mass spectrometry, reverse immunoprecipitation, and structured illumination microscopy in T. gondii\",\n      \"pmids\": [\"28138739\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"NUP67 was identified only within a bulk interaction screen; no functional experiments were performed on NUP67 itself\",\n        \"Direct NPC localization of NUP67 (as opposed to the complex) was not independently demonstrated\",\n        \"The specific structural or transport role of NUP67 within the NPC is undefined\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function, transport role, and interaction stoichiometry of NUP67 within the Toxoplasma NPC remain unestablished.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No functional assay on NUP67 itself\",\n        \"No structural model of NUP67 within the NPC\",\n        \"Mechanism of NPC assembly or nucleocytoplasmic transport contribution unknown\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0005643\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"Toxoplasma gondii nuclear pore complex (TgNup302 interaction network)\"],\n    \"partners\": [\"TgNup302\"],\n    \"other_free_text\": [\"Identified as a TgNup302-interacting protein by affinity purification-mass spectrometry; no NUP67-specific functional characterization available.\"]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_306560","name":"NUP67","product":"nucleoporin NUP67, putative","uniprot_acc":"A0A125YIF1","chromosome":"TGME49_chrIX","start_pos":6180349},{"strain":"GT1","gene_id":"TGGT1_306560","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":5962419},{"strain":"RH88","gene_id":"TGRH88_018010","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023090","start_pos":6304876},{"strain":"VEG","gene_id":"TGVEG_306560","name":null,"product":"nucleoporin FG repeat region protein","uniprot_acc":"B6KRE5","chromosome":"TGVEG_chrIX","start_pos":6221153}]}