IMC20

IMC20 is a component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity-dependent biotinylation as residing within the IMC suborganellar compartment (alveoli or cytoskeletal network) (PMID:25691595). Beyond this proximity-based localization, no further mechanistic detail — molecular function or binding partners — has been characterized in the available corpus.

1. 2015 Medium

   Establishing the protein composition of the Toxoplasma IMC required an unbiased spatial proteomics approach; BioID placed IMC20 among the constituents of the IMC suborganellar compartment, defining it as a bona fide IMC-resident protein.

   Evidence BioID proximity-dependent biotinylation followed by mass spectrometry in T. gondii

   PMID:25691595

   Open questions at the time

   • IMC20 was one of 19 newly discovered proteins and was not functionally evaluated by gene knockout
   • specific molecular function not determined
   • binding partners and sub-compartment assignment (alveoli vs cytoskeletal network) not resolved