{"gene":"TG_271930","gene_symbol":"IMC20","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"IMC20 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) through BioID proximity-dependent biotinylation, placing it within the IMC suborganellar compartment (alveoli or cytoskeletal network).","method":"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry identification of biotinylated proteins","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID is a proximity-labeling method with functional validation by localization, but IMC20 was one of 19 newly discovered proteins and was not itself one of the two functionally evaluated by gene knockout; single lab, single method for this specific protein.","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC20 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling as residing within the IMC suborganellar compartments, but its specific molecular function and binding partners remain uncharacterized."},"narrative":{"mechanistic_narrative":"IMC20 is a component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity-dependent biotinylation as residing within the IMC suborganellar compartment (alveoli or cytoskeletal network) [PMID:25691595]. Beyond this proximity-based localization, no further mechanistic detail — molecular function or binding partners — has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"Establishing the protein composition of the Toxoplasma IMC required an unbiased spatial proteomics approach; BioID placed IMC20 among the constituents of the IMC suborganellar compartment, defining it as a bona fide IMC-resident protein.","evidence":"BioID proximity-dependent biotinylation followed by mass spectrometry in T. gondii","pmids":["25691595"],"confidence":"Medium","gaps":["IMC20 was one of 19 newly discovered proteins and was not functionally evaluated by gene knockout","specific molecular function not determined","binding partners and sub-compartment assignment (alveoli vs cytoskeletal network) not resolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["inner membrane complex (IMC)"],"partners":[],"other_free_text":["Identified via BioID proximity labeling as an IMC-resident protein; sub-compartment (alveoli vs cytoskeletal network) not resolved."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3086,"output_tokens":347,"usd":0.007232,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4931,"output_tokens":921,"usd":0.02384,"stage2_stop_reason":"end_turn"},"total_usd":0.032811,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"IMC20 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) through BioID proximity-dependent biotinylation, placing it within the IMC suborganellar compartment (alveoli or cytoskeletal network).\",\n      \"method\": \"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry identification of biotinylated proteins\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID is a proximity-labeling method with functional validation by localization, but IMC20 was one of 19 newly discovered proteins and was not itself one of the two functionally evaluated by gene knockout; single lab, single method for this specific protein.\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC20 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling as residing within the IMC suborganellar compartments, but its specific molecular function and binding partners remain uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC20 is a component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity-dependent biotinylation as residing within the IMC suborganellar compartment (alveoli or cytoskeletal network) [#0]. Beyond this proximity-based localization, no further mechanistic detail — molecular function or binding partners — has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"Establishing the protein composition of the Toxoplasma IMC required an unbiased spatial proteomics approach; BioID placed IMC20 among the constituents of the IMC suborganellar compartment, defining it as a bona fide IMC-resident protein.\",\n      \"evidence\": \"BioID proximity-dependent biotinylation followed by mass spectrometry in T. gondii\",\n      \"pmids\": [\n        \"25691595\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"IMC20 was one of 19 newly discovered proteins and was not functionally evaluated by gene knockout\",\n        \"specific molecular function not determined\",\n        \"binding partners and sub-compartment assignment (alveoli vs cytoskeletal network) not resolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular activity, direct binding partners, and functional contribution of IMC20 to IMC biology remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"no biochemical activity assigned\",\n        \"no direct interaction partners identified\",\n        \"no loss-of-function phenotype established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0070258\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified via BioID proximity labeling as an IMC-resident protein; sub-compartment (alveoli vs cytoskeletal network) not resolved.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_271930","name":"IMC20","product":"inner membrane complex protein IMC20","uniprot_acc":"S8EYN9","chromosome":"TGME49_chrVIII","start_pos":4277188},{"strain":"GT1","gene_id":"TGGT1_271930","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":4274565},{"strain":"RH88","gene_id":"TGRH88_079680","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":9385999},{"strain":"VEG","gene_id":"TGVEG_271930","name":null,"product":"hypothetical protein","uniprot_acc":"B9Q6D5","chromosome":"TGVEG_chrVIII","start_pos":4286701}]}