NUP37

NUP37 (TG_248500) has been identified as a component of the Toxoplasma gondii nuclear pore complex, recovered among the proteins co-purifying with the nucleoporin TgNup302 by affinity tag purification and mass spectrometry, with NPC localization confirmed for some of the co-purifying nucleoporins by structured illumination microscopy (PMID:28138739). Beyond this identification as an NPC-associated protein, nothing further about NUP37 function has been characterized in the available corpus.

1. 2017 Low

   Before this work it was unknown whether Toxoplasma encodes a conserved NPC subunit corresponding to NUP37; co-purification with TgNup302 placed NUP37 within the parasite's nuclear pore complex interactome, establishing it as a candidate structural NPC component.

   Evidence Affinity tag purification and mass spectrometry of TgNup302 interactors, with reverse immunoprecipitation and structured illumination microscopy of NPC localization

   PMID:28138739

   Open questions at the time

   • NUP37 was identified only as a co-purifying protein in a single AP-MS screen, without reciprocal validation targeting NUP37 directly
   • No direct functional experiments, localization, or interaction mapping specific to NUP37 were performed
   • The stoichiometry and position of NUP37 within the NPC architecture are undefined