{"gene":"TG_248500","gene_symbol":"NUP37","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2017,"finding":"The Toxoplasma gondii nuclear pore complex (NPC), which includes NUP37 (TG_248500) as an identified component, was partially characterized via affinity tag purification and mass spectrometry of TgNup302-interacting proteins; NUP37 was among the NPC proteins identified in this interactome, and structured illuminated microscopy confirmed NPC localization of some TgNup302-interacting proteins.","method":"Affinity tag purification combined with mass spectrometry (AP-MS); reverse immunoprecipitation; structured illumination microscopy","journal":"Cellular and molecular life sciences : CMLS","confidence":"Low","confidence_rationale":"Tier 3 / Weak — NUP37 identified as a component in a co-purification/MS screen of TgNup302 interactors; no direct functional experiments performed on NUP37 itself; single lab, single method for NUP37 identification","pmids":["28138739"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"NUP37 (TG_248500) has been identified as a component of the Toxoplasma gondii nuclear pore complex through co-purification mass spectrometry of the nucleoporin TgNup302, suggesting structural conservation of NPC subunits in Apicomplexa, but no direct functional experiments on NUP37 itself have been reported."},"narrative":{"mechanistic_narrative":"NUP37 (TG_248500) has been identified as a component of the Toxoplasma gondii nuclear pore complex, recovered among the proteins co-purifying with the nucleoporin TgNup302 by affinity tag purification and mass spectrometry, with NPC localization confirmed for some of the co-purifying nucleoporins by structured illumination microscopy [PMID:28138739]. Beyond this identification as an NPC-associated protein, nothing further about NUP37 function has been characterized in the available corpus.","teleology":[{"year":2017,"claim":"Before this work it was unknown whether Toxoplasma encodes a conserved NPC subunit corresponding to NUP37; co-purification with TgNup302 placed NUP37 within the parasite's nuclear pore complex interactome, establishing it as a candidate structural NPC component.","evidence":"Affinity tag purification and mass spectrometry of TgNup302 interactors, with reverse immunoprecipitation and structured illumination microscopy of NPC localization","pmids":["28138739"],"confidence":"Low","gaps":["NUP37 was identified only as a co-purifying protein in a single AP-MS screen, without reciprocal validation targeting NUP37 directly","No direct functional experiments, localization, or interaction mapping specific to NUP37 were performed","The stoichiometry and position of NUP37 within the NPC architecture are undefined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Nuclear pore complex (NPC)"],"partners":["TGNUP302"],"other_free_text":["NUP37 identified as a component of the Toxoplasma gondii NPC via co-purification with TgNup302; no direct functional data reported."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"28138739","id":"PMC_28138739","title":"Characterization of a nuclear pore protein sheds light on the roles and composition of the Toxoplasma gondii nuclear pore complex.","date":"2017","source":"Cellular and molecular life sciences : CMLS","url":"https://pubmed.ncbi.nlm.nih.gov/28138739","citation_count":13,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2996,"output_tokens":1121,"usd":0.012901,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6119,"output_tokens":2229,"usd":0.04316,"stage2_stop_reason":"end_turn"},"total_usd":0.091101,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"The Toxoplasma gondii nuclear pore complex (NPC), which includes NUP37 (TG_248500) as an identified component, was partially characterized via affinity tag purification and mass spectrometry of TgNup302-interacting proteins; NUP37 was among the NPC proteins identified in this interactome, and structured illuminated microscopy confirmed NPC localization of some TgNup302-interacting proteins.\",\n      \"method\": \"Affinity tag purification combined with mass spectrometry (AP-MS); reverse immunoprecipitation; structured illumination microscopy\",\n      \"journal\": \"Cellular and molecular life sciences : CMLS\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — NUP37 identified as a component in a co-purification/MS screen of TgNup302 interactors; no direct functional experiments performed on NUP37 itself; single lab, single method for NUP37 identification\",\n      \"pmids\": [\"28138739\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"NUP37 (TG_248500) has been identified as a component of the Toxoplasma gondii nuclear pore complex through co-purification mass spectrometry of the nucleoporin TgNup302, suggesting structural conservation of NPC subunits in Apicomplexa, but no direct functional experiments on NUP37 itself have been reported.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"NUP37 (TG_248500) has been identified as a component of the Toxoplasma gondii nuclear pore complex, recovered among the proteins co-purifying with the nucleoporin TgNup302 by affinity tag purification and mass spectrometry, with NPC localization confirmed for some of the co-purifying nucleoporins by structured illumination microscopy [#0]. Beyond this identification as an NPC-associated protein, nothing further about NUP37 function has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"Before this work it was unknown whether Toxoplasma encodes a conserved NPC subunit corresponding to NUP37; co-purification with TgNup302 placed NUP37 within the parasite's nuclear pore complex interactome, establishing it as a candidate structural NPC component.\",\n      \"evidence\": \"Affinity tag purification and mass spectrometry of TgNup302 interactors, with reverse immunoprecipitation and structured illumination microscopy of NPC localization\",\n      \"pmids\": [\n        \"28138739\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"NUP37 was identified only as a co-purifying protein in a single AP-MS screen, without reciprocal validation targeting NUP37 directly\",\n        \"No direct functional experiments, localization, or interaction mapping specific to NUP37 were performed\",\n        \"The stoichiometry and position of NUP37 within the NPC architecture are undefined\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The functional role, essentiality, and precise molecular activity of NUP37 within the Toxoplasma nuclear pore complex remain unknown.\",\n      \"evidence\": \"No further discoveries in the timeline\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No direct functional characterization of NUP37 exists\",\n        \"No structural placement within the NPC is defined\",\n        \"No genetic or phenotypic data on NUP37 are available\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0005643\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Nuclear pore complex (NPC)\"\n    ],\n    \"partners\": [\n      \"TgNup302\"\n    ],\n    \"other_free_text\": [\n      \"NUP37 identified as a component of the Toxoplasma gondii NPC via co-purification with TgNup302; no direct functional data reported.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_248500","name":"NUP37","product":"nucleoporin NUP37","uniprot_acc":"A0A125YJ23","chromosome":"TGME49_chrXII","start_pos":3890613},{"strain":"GT1","gene_id":"TGGT1_248500","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrXII","start_pos":3823885},{"strain":"RH88","gene_id":"TGRH88_062380","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023093","start_pos":3944465},{"strain":"VEG","gene_id":"TGVEG_248500","name":null,"product":"nucleoporin complex subunit 54","uniprot_acc":"B6KH74","chromosome":"TGVEG_chrXII","start_pos":3862193}]}