TSC5

TSC5 is a component of the Toxoplasma gondii inner membrane complex (IMC) that localizes exclusively to the transverse sutures, one of five novel proteins defining a distinct suture subcompartment (the TSCs) separate from ISC proteins that span both transverse and longitudinal sutures (PMID:27696623). It was identified by proximity-dependent biotin identification (BioID) using ISC4 as bait (PMID:27696623). Beyond this localization within the transverse-suture subcompartment, no further mechanistic detail about TSC5 has been characterized in the available corpus.

1. 2016 Medium

   Whether the Toxoplasma IMC sutures contain compartment-specific proteins beyond the previously known ISCs was unknown; BioID from ISC4 resolved a distinct transverse-suture protein set, placing TSC5 in a spatially defined IMC subcompartment.

   Evidence Proximity-dependent biotin identification (BioID) with ISC4 as bait followed by localization studies in Toxoplasma gondii

   PMID:27696623

   Open questions at the time

   • No molecular function or biochemical activity for TSC5 established
   • Direct binding partners and how TSC5 is targeted specifically to transverse sutures unknown
   • No loss-of-function or phenotypic characterization reported