{"gene":"TG_232260","gene_symbol":"TSC5","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2016,"finding":"TSC5 is one of five novel proteins that exclusively localize to the transverse sutures of the Toxoplasma IMC, identified by proximity-dependent biotin identification (BioID) using ISC4 as bait, establishing a distinct subcompartment of proteins (TSCs) separate from ISCs that span both transverse and longitudinal sutures.","method":"Proximity-dependent biotin identification (BioID) with ISC4 as bait, followed by localization studies","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID proximity labeling with follow-up localization, single lab, single study","pmids":["27696623"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TSC5 is a component of the Toxoplasma inner membrane complex (IMC) that localizes exclusively to the transverse sutures, distinguishing it from ISC proteins that span both transverse and longitudinal sutures, as identified by BioID proximity labeling."},"narrative":{"mechanistic_narrative":"TSC5 is a component of the Toxoplasma gondii inner membrane complex (IMC) that localizes exclusively to the transverse sutures, one of five novel proteins defining a distinct suture subcompartment (the TSCs) separate from ISC proteins that span both transverse and longitudinal sutures [PMID:27696623]. It was identified by proximity-dependent biotin identification (BioID) using ISC4 as bait [PMID:27696623]. Beyond this localization within the transverse-suture subcompartment, no further mechanistic detail about TSC5 has been characterized in the available corpus.","teleology":[{"year":2016,"claim":"Whether the Toxoplasma IMC sutures contain compartment-specific proteins beyond the previously known ISCs was unknown; BioID from ISC4 resolved a distinct transverse-suture protein set, placing TSC5 in a spatially defined IMC subcompartment.","evidence":"Proximity-dependent biotin identification (BioID) with ISC4 as bait followed by localization studies in Toxoplasma gondii","pmids":["27696623"],"confidence":"Medium","gaps":["No molecular function or biochemical activity for TSC5 established","Direct binding partners and how TSC5 is targeted specifically to transverse sutures unknown","No loss-of-function or phenotypic characterization reported"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma inner membrane complex (IMC), transverse suture subcompartment (TSCs)"],"partners":["ISC4 (BIOID BAIT USED TO IDENTIFY TSC5)"],"other_free_text":["TSC5 is one of five proteins defining the transverse-suture (TSC) subcompartment, distinct from ISC proteins that span both transverse and longitudinal sutures."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"27696623","id":"PMC_27696623","title":"Novel insights into the composition and function of the Toxoplasma IMC sutures.","date":"2016","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/27696623","citation_count":59,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2890,"output_tokens":307,"usd":0.006638,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4882,"output_tokens":1064,"usd":0.025505,"stage2_stop_reason":"end_turn"},"total_usd":0.066287,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2016,\n      \"finding\": \"TSC5 is one of five novel proteins that exclusively localize to the transverse sutures of the Toxoplasma IMC, identified by proximity-dependent biotin identification (BioID) using ISC4 as bait, establishing a distinct subcompartment of proteins (TSCs) separate from ISCs that span both transverse and longitudinal sutures.\",\n      \"method\": \"Proximity-dependent biotin identification (BioID) with ISC4 as bait, followed by localization studies\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID proximity labeling with follow-up localization, single lab, single study\",\n      \"pmids\": [\"27696623\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"TSC5 is a component of the Toxoplasma inner membrane complex (IMC) that localizes exclusively to the transverse sutures, distinguishing it from ISC proteins that span both transverse and longitudinal sutures, as identified by BioID proximity labeling.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"TSC5 is a component of the Toxoplasma gondii inner membrane complex (IMC) that localizes exclusively to the transverse sutures, one of five novel proteins defining a distinct suture subcompartment (the TSCs) separate from ISC proteins that span both transverse and longitudinal sutures [#0]. It was identified by proximity-dependent biotin identification (BioID) using ISC4 as bait [#0]. Beyond this localization within the transverse-suture subcompartment, no further mechanistic detail about TSC5 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2016,\n      \"claim\": \"Whether the Toxoplasma IMC sutures contain compartment-specific proteins beyond the previously known ISCs was unknown; BioID from ISC4 resolved a distinct transverse-suture protein set, placing TSC5 in a spatially defined IMC subcompartment.\",\n      \"evidence\": \"Proximity-dependent biotin identification (BioID) with ISC4 as bait followed by localization studies in Toxoplasma gondii\",\n      \"pmids\": [\n        \"27696623\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No molecular function or biochemical activity for TSC5 established\",\n        \"Direct binding partners and how TSC5 is targeted specifically to transverse sutures unknown\",\n        \"No loss-of-function or phenotypic characterization reported\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of TSC5, its interaction partners, and its role in IMC assembly or parasite biology remain uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No enzymatic or structural activity assigned\",\n        \"No functional genetic disruption or phenotype\",\n        \"Mechanism of transverse-suture-specific targeting unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0070258\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Toxoplasma inner membrane complex (IMC), transverse suture subcompartment (TSCs)\"\n    ],\n    \"partners\": [\n      \"ISC4 (BioID bait used to identify TSC5)\"\n    ],\n    \"other_free_text\": [\n      \"TSC5 is one of five proteins defining the transverse-suture (TSC) subcompartment, distinct from ISC proteins that span both transverse and longitudinal sutures.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_232260","name":"TSC5","product":"transverse sutures component TSC5","uniprot_acc":"A0A125YGI5","chromosome":"TGME49_chrVIII","start_pos":1982806},{"strain":"GT1","gene_id":"TGGT1_232260","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":1978812},{"strain":"RH88","gene_id":"TGRH88_076390","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":7089125},{"strain":"VEG","gene_id":"TGVEG_232260","name":null,"product":"putative transmembrane protein","uniprot_acc":"B9PGK8","chromosome":"TGVEG_chrVIII","start_pos":1989858}]}