IMC21

IMC21 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified through BioID proximity labeling using established IMC proteins as bait, which places it within the alveolar or cytoskeletal subcompartment of the IMC (PMID:25691595). Beyond this proximity-based assignment, no molecular function, interaction, or phenotype for IMC21 has been characterized in the available corpus.

1. 2015 Low

   Whether additional uncharacterized proteins reside in the Toxoplasma IMC was unresolved; proximity labeling with known IMC baits placed IMC21 in the alveolar or cytoskeletal subcompartment, establishing it as a bona fide IMC-resident protein.

   Evidence BioID proximity-dependent biotin identification in T. gondii with IMC-protein baits, followed by mass spectrometry

   PMID:25691595

   Open questions at the time

   • Single proximity-labeling screen; IMC21 was not among the proteins functionally evaluated by knockout
   • No direct demonstration of localization by imaging for IMC21 specifically
   • No molecular activity, binding partners, or phenotype established