{"gene":"TG_232030","gene_symbol":"IMC21","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"IMC21 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with established IMC proteins as bait, placing it within the alveolar or cytoskeletal subcompartment of the IMC.","method":"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry identification of biotinylated proteins","journal":"mBio","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single proximity-labeling screen; IMC21 is listed among 19 novel IMC proteins but is not one of the two functionally evaluated by knockout; no direct functional characterization of IMC21 itself is described","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC21 is a novel inner membrane complex protein of Toxoplasma gondii identified by BioID proximity labeling, localizing to the IMC alveolar or cytoskeletal network, but its specific molecular function has not yet been experimentally characterized."},"narrative":{"mechanistic_narrative":"IMC21 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified through BioID proximity labeling using established IMC proteins as bait, which places it within the alveolar or cytoskeletal subcompartment of the IMC [PMID:25691595]. Beyond this proximity-based assignment, no molecular function, interaction, or phenotype for IMC21 has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"Whether additional uncharacterized proteins reside in the Toxoplasma IMC was unresolved; proximity labeling with known IMC baits placed IMC21 in the alveolar or cytoskeletal subcompartment, establishing it as a bona fide IMC-resident protein.","evidence":"BioID proximity-dependent biotin identification in T. gondii with IMC-protein baits, followed by mass spectrometry","pmids":["25691595"],"confidence":"Low","gaps":["Single proximity-labeling screen; IMC21 was not among the proteins functionally evaluated by knockout","No direct demonstration of localization by imaging for IMC21 specifically","No molecular activity, binding partners, or phenotype established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Inner membrane complex (IMC)"],"partners":[],"other_free_text":["Assigned to the alveolar or cytoskeletal subcompartment of the IMC by BioID proximity labeling [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3086,"output_tokens":328,"usd":0.007089,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4902,"output_tokens":929,"usd":0.023867,"stage2_stop_reason":"end_turn"},"total_usd":0.033213,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"IMC21 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with established IMC proteins as bait, placing it within the alveolar or cytoskeletal subcompartment of the IMC.\",\n      \"method\": \"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry identification of biotinylated proteins\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single proximity-labeling screen; IMC21 is listed among 19 novel IMC proteins but is not one of the two functionally evaluated by knockout; no direct functional characterization of IMC21 itself is described\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC21 is a novel inner membrane complex protein of Toxoplasma gondii identified by BioID proximity labeling, localizing to the IMC alveolar or cytoskeletal network, but its specific molecular function has not yet been experimentally characterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC21 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified through BioID proximity labeling using established IMC proteins as bait, which places it within the alveolar or cytoskeletal subcompartment of the IMC [#0]. Beyond this proximity-based assignment, no molecular function, interaction, or phenotype for IMC21 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"Whether additional uncharacterized proteins reside in the Toxoplasma IMC was unresolved; proximity labeling with known IMC baits placed IMC21 in the alveolar or cytoskeletal subcompartment, establishing it as a bona fide IMC-resident protein.\",\n      \"evidence\": \"BioID proximity-dependent biotin identification in T. gondii with IMC-protein baits, followed by mass spectrometry\",\n      \"pmids\": [\n        \"25691595\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single proximity-labeling screen; IMC21 was not among the proteins functionally evaluated by knockout\",\n        \"No direct demonstration of localization by imaging for IMC21 specifically\",\n        \"No molecular activity, binding partners, or phenotype established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of IMC21, its direct interaction partners, and its contribution to parasite biology remain unknown.\",\n      \"evidence\": \"No further experimental characterization present in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No knockout or functional assay for IMC21\",\n        \"No defined enzymatic or structural role\",\n        \"No identified binding partners within the IMC\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0070258\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Assigned to the alveolar or cytoskeletal subcompartment of the IMC by BioID proximity labeling [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_232030","name":"IMC21","product":"inner membrane complex protein IMC21","uniprot_acc":"A0A125YM25","chromosome":"TGME49_chrVIII","start_pos":1772693},{"strain":"GT1","gene_id":"TGGT1_232030","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":1768528},{"strain":"RH88","gene_id":"TGRH88_076150","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":6878843},{"strain":"VEG","gene_id":"TGVEG_232030","name":null,"product":"hypothetical protein","uniprot_acc":"B9Q4U5","chromosome":"TGVEG_chrVIII","start_pos":1779398}]}