Affinage · Toxoplasma gondii

BCC2

basal complex component BCC2 · UniProt S8F667

Annotated
2026-09-10
2 papers in source corpus 2 papers cited in narrative 2 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

BCC2 is a component of the basal complex of Toxoplasma gondii, localizing to a defined sub-compartment of this cytoskeletal structure as shown by spatial proteomics and confirmed by in situ ultrastructure expansion microscopy (PMID:36310866). Functional disruption established that BCC2 is non-essential for parasite growth in vitro (PMID:36310866). The basal complex proteome to which BCC2 belongs comprises four architecturally distinct subcomplexes whose compositions change dynamically with basal complex function, and BCC2 is placed within this expanded hierarchy (PMID:35941170). Beyond its localization and dispensability for in vitro growth, no specific molecular function for BCC2 has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 2022 Medium

    Establishing where BCC2 acts and whether it is required for growth was the first step in defining its role; spatial proteomics placed it within a sub-compartment of the basal complex and disruption showed it is dispensable for in vitro replication.

    Evidence Proximity-based biotinylation (BioID), ultrastructure expansion microscopy, and functional disruption assays in Toxoplasma gondii

    PMID:36310866

    Open questions at the time
    • No molecular activity or binding partners of BCC2 were defined
    • Non-essentiality in vitro leaves any role in vivo or under stress untested
    • The functional consequence of its specific sub-compartment localization is unknown
  2. 2022 Low

    Mapping the basal complex proteome addressed how BCC2 fits into overall basal complex organization, placing it within one of four dynamically changing subcomplexes rather than a single static structure.

    Evidence Reciprocal proximity-based biotinylation (BioID) proteomics with expression disruption in Toxoplasma gondii

    PMID:35941170

    Open questions at the time
    • No experiment specific to BCC2 itself is reported in this proteome-level map
    • Which subcomplex BCC2 resides in and its interactions there are not resolved
    • The mechanistic driver of the dynamic compositional changes is not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification
Complex memberships
Toxoplasma gondii basal complex

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2022 BCC2 was identified as a basal complex component (BCC) by spatial proteomics and its localization was confirmed by in situ imaging, including ultrastructure expansion microscopy, positioning it within a sub-compartment of the basal complex. BCC2 was found to be non-essential for parasite growth in vitro. Spatial proteomics (proximity-based biotinylation / BioID), ultrastructure expansion microscopy, functional disruption assays Frontiers in cellular and infection microbiology Medium 36310866
2022 BCC2 was identified as a component of the basal complex (BC) proteome through a reciprocal proximity-based biotinylation approach. The BC proteome study identified four architecturally distinct BC subcomplexes whose compositions change dynamically with BC function, and BCC2 is placed within this expanded hierarchy. Reciprocal proximity-based biotinylation (BioID) proteomics, expression disruption Nature communications Low 35941170

Source papers

Stage 0 corpus · 2 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2022 Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function. Nature communications 46 35941170
2022 Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions. Frontiers in cellular and infection microbiology 20 36310866