{"gene":"TG_231070","gene_symbol":"BCC2","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"BCC2 was identified as a basal complex component (BCC) by spatial proteomics and its localization was confirmed by in situ imaging, including ultrastructure expansion microscopy, positioning it within a sub-compartment of the basal complex. BCC2 was found to be non-essential for parasite growth in vitro.","method":"Spatial proteomics (proximity-based biotinylation / BioID), ultrastructure expansion microscopy, functional disruption assays","journal":"Frontiers in cellular and infection microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — spatial proteomics with imaging confirmation in a single study; localization established but functional role limited to a non-essential growth phenotype without further mechanistic detail specific to BCC2","pmids":["36310866"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2022,"finding":"BCC2 was identified as a component of the basal complex (BC) proteome through a reciprocal proximity-based biotinylation approach. The BC proteome study identified four architecturally distinct BC subcomplexes whose compositions change dynamically with BC function, and BCC2 is placed within this expanded hierarchy.","method":"Reciprocal proximity-based biotinylation (BioID) proteomics, expression disruption","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — BCC2 is listed as part of a proteome-level BC map with no experiment specific to BCC2 itself reported in this abstract","pmids":["35941170"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"BCC2 is a basal complex component of Toxoplasma gondii that localizes to a sub-compartment of the basal complex, is non-essential for in vitro parasite growth, and is part of a dynamic multi-subcomplex basal complex architecture identified by spatial proteomics, but its specific molecular function remains undefined."},"narrative":{"mechanistic_narrative":"BCC2 is a component of the basal complex of Toxoplasma gondii, localizing to a defined sub-compartment of this cytoskeletal structure as shown by spatial proteomics and confirmed by in situ ultrastructure expansion microscopy [PMID:36310866]. Functional disruption established that BCC2 is non-essential for parasite growth in vitro [PMID:36310866]. The basal complex proteome to which BCC2 belongs comprises four architecturally distinct subcomplexes whose compositions change dynamically with basal complex function, and BCC2 is placed within this expanded hierarchy [PMID:35941170]. Beyond its localization and dispensability for in vitro growth, no specific molecular function for BCC2 has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Establishing where BCC2 acts and whether it is required for growth was the first step in defining its role; spatial proteomics placed it within a sub-compartment of the basal complex and disruption showed it is dispensable for in vitro replication.","evidence":"Proximity-based biotinylation (BioID), ultrastructure expansion microscopy, and functional disruption assays in Toxoplasma gondii","pmids":["36310866"],"confidence":"Medium","gaps":["No molecular activity or binding partners of BCC2 were defined","Non-essentiality in vitro leaves any role in vivo or under stress untested","The functional consequence of its specific sub-compartment localization is unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2022,"claim":"Mapping the basal complex proteome addressed how BCC2 fits into overall basal complex organization, placing it within one of four dynamically changing subcomplexes rather than a single static structure.","evidence":"Reciprocal proximity-based biotinylation (BioID) proteomics with expression disruption in Toxoplasma gondii","pmids":["35941170"],"confidence":"Low","gaps":["No experiment specific to BCC2 itself is reported in this proteome-level map","Which subcomplex BCC2 resides in and its interactions there are not resolved","The mechanistic driver of the dynamic compositional changes is not defined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii basal complex"],"partners":[],"other_free_text":["BCC2 is non-essential for Toxoplasma gondii growth in vitro [#0]","BCC2 is placed within a basal complex hierarchy of four architecturally distinct, dynamically changing subcomplexes [#1]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"35941170","id":"PMC_35941170","title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.","date":"2022","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/35941170","citation_count":46,"is_preprint":false},{"pmid":"36310866","id":"PMC_36310866","title":"Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions.","date":"2022","source":"Frontiers in cellular and infection microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/36310866","citation_count":20,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3286,"output_tokens":1660,"usd":0.017379,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6883,"output_tokens":2041,"usd":0.04272,"stage2_stop_reason":"end_turn"},"total_usd":0.100778,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"BCC2 was identified as a basal complex component (BCC) by spatial proteomics and its localization was confirmed by in situ imaging, including ultrastructure expansion microscopy, positioning it within a sub-compartment of the basal complex. BCC2 was found to be non-essential for parasite growth in vitro.\",\n      \"method\": \"Spatial proteomics (proximity-based biotinylation / BioID), ultrastructure expansion microscopy, functional disruption assays\",\n      \"journal\": \"Frontiers in cellular and infection microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — spatial proteomics with imaging confirmation in a single study; localization established but functional role limited to a non-essential growth phenotype without further mechanistic detail specific to BCC2\",\n      \"pmids\": [\"36310866\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": 2022,\n      \"finding\": \"BCC2 was identified as a component of the basal complex (BC) proteome through a reciprocal proximity-based biotinylation approach. The BC proteome study identified four architecturally distinct BC subcomplexes whose compositions change dynamically with BC function, and BCC2 is placed within this expanded hierarchy.\",\n      \"method\": \"Reciprocal proximity-based biotinylation (BioID) proteomics, expression disruption\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — BCC2 is listed as part of a proteome-level BC map with no experiment specific to BCC2 itself reported in this abstract\",\n      \"pmids\": [\"35941170\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"BCC2 is a basal complex component of Toxoplasma gondii that localizes to a sub-compartment of the basal complex, is non-essential for in vitro parasite growth, and is part of a dynamic multi-subcomplex basal complex architecture identified by spatial proteomics, but its specific molecular function remains undefined.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"BCC2 is a component of the basal complex of Toxoplasma gondii, localizing to a defined sub-compartment of this cytoskeletal structure as shown by spatial proteomics and confirmed by in situ ultrastructure expansion microscopy [#0]. Functional disruption established that BCC2 is non-essential for parasite growth in vitro [#0]. The basal complex proteome to which BCC2 belongs comprises four architecturally distinct subcomplexes whose compositions change dynamically with basal complex function, and BCC2 is placed within this expanded hierarchy [#1]. Beyond its localization and dispensability for in vitro growth, no specific molecular function for BCC2 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Establishing where BCC2 acts and whether it is required for growth was the first step in defining its role; spatial proteomics placed it within a sub-compartment of the basal complex and disruption showed it is dispensable for in vitro replication.\",\n      \"evidence\": \"Proximity-based biotinylation (BioID), ultrastructure expansion microscopy, and functional disruption assays in Toxoplasma gondii\",\n      \"pmids\": [\"36310866\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No molecular activity or binding partners of BCC2 were defined\",\n        \"Non-essentiality in vitro leaves any role in vivo or under stress untested\",\n        \"The functional consequence of its specific sub-compartment localization is unknown\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2022,\n      \"claim\": \"Mapping the basal complex proteome addressed how BCC2 fits into overall basal complex organization, placing it within one of four dynamically changing subcomplexes rather than a single static structure.\",\n      \"evidence\": \"Reciprocal proximity-based biotinylation (BioID) proteomics with expression disruption in Toxoplasma gondii\",\n      \"pmids\": [\"35941170\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No experiment specific to BCC2 itself is reported in this proteome-level map\",\n        \"Which subcomplex BCC2 resides in and its interactions there are not resolved\",\n        \"The mechanistic driver of the dynamic compositional changes is not defined\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of BCC2 within the basal complex remains undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No biochemical activity identified for BCC2\",\n        \"No direct binding partners of BCC2 established\",\n        \"No structural or mechanistic role within its basal complex sub-compartment defined\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"basal complex sub-compartment\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"Toxoplasma gondii basal complex\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"BCC2 is non-essential for Toxoplasma gondii growth in vitro [#0]\",\n      \"BCC2 is placed within a basal complex hierarchy of four architecturally distinct, dynamically changing subcomplexes [#1]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_231070","name":"BCC2","product":"basal complex component BCC2","uniprot_acc":"S8F667","chromosome":"TGME49_chrVIII","start_pos":1222675},{"strain":"GT1","gene_id":"TGGT1_231070","name":null,"product":"protein kinase","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":1218431},{"strain":"RH88","gene_id":"TGRH88_075390","name":null,"product":"protein kinase","uniprot_acc":null,"chromosome":"CM023089","start_pos":6329964},{"strain":"VEG","gene_id":"TGVEG_231070","name":null,"product":"protein kinase","uniprot_acc":"V4ZUB4","chromosome":"TGVEG_chrVIII","start_pos":1229519}]}