Affinage · Toxoplasma gondii

RPL40

ribosomal-ubiquitin protein RPL40 · UniProt A0A125YNY5

Annotated
2026-09-08
1 papers in source corpus 1 papers cited in narrative 2 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

TgRPL40 (ribosomal-ubiquitin protein L40) of Toxoplasma gondii is a tachyzoite antigen that is broadly distributed throughout the parasite and is recovered in both circulating antigen and excretory/secretory antigen (ESA) fractions (PMID:31592775). Its presence in ESA and circulating fractions was established by Western blotting with an antibody raised against recombinant TgRPL40, and its parasite-wide distribution across the tachyzoite was confirmed by immunofluorescence and immunoelectron microscopy (PMID:31592775). Beyond this antigenic localization and secretion profile, no molecular activity, binding partners, or mechanistic role for TgRPL40 has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 2019 Medium

    Whether TgRPL40 is confined intracellularly or reaches the parasite secretome was unknown; detecting it in circulating and excretory/secretory antigen fractions established it as a released/circulating antigen relevant to host exposure.

    Evidence Western blotting of circulating antigen and ESA fractions with anti-recombinant TgRPL40 antibody

    PMID:31592775

    Open questions at the time
    • Mechanism and route of secretion not defined
    • No functional or enzymatic role established for the protein
    • Strain not specified
  2. 2019 Medium

    The subcellular and stage distribution of TgRPL40 was unknown; imaging showed it is broadly distributed throughout the tachyzoite, consistent with an abundant, non-compartment-restricted antigen.

    Evidence Immunofluorescence and immunoelectron microscopy with anti-TgRPL40 antibody in tachyzoites

    PMID:31592775

    Open questions at the time
    • No specific organellar assignment resolved
    • Distribution in other life-cycle stages not examined
    • No link between localization and function established

Open questions

Synthesis pass · forward-looking unresolved questions
  • The molecular function of TgRPL40, its role in the ribosome or ubiquitin pathway, its binding partners, and the basis of any protective immunity it confers remain uncharacterized in the available corpus.
  • No enzymatic or ribosomal activity demonstrated experimentally
  • No interaction partners or complex membership identified
  • Mechanism of immune protection not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 TgRPL40 (ribosomal-ubiquitin protein L40) is present in circulating antigens and excretory/secretory antigens (ESA) of Toxoplasma gondii, as determined by Western blotting with antibody prepared against recombinant TgRPL40. Western blotting of circulating antigens and ESA fractions using anti-recombinant TgRPL40 antibody Folia parasitologica Medium 31592775
2019 TgRPL40 protein is widely distributed throughout the tachyzoite stage of T. gondii, as revealed by immunofluorescence and immunoelectron microscopy. Immunofluorescence microscopy and immunoelectron microscopy with anti-TgRPL40 antibody Folia parasitologica Medium 31592775

Source papers

Stage 0 corpus · 1 paper · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 Toxoplasma gondii RPL40 is a circulating antigen with immune protection effect. Folia parasitologica 3 31592775