Affinage · Toxoplasma gondii

PFK1

6-phosphofructokinase · UniProt A0A125YZ29

Annotated
2026-09-10
1 papers in source corpus 1 papers cited in narrative 2 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

In Toxoplasma gondii, PFK1 is an ATP-dependent phosphofructokinase (ATP-PFK) expressed in the cytoplasm alongside a distinct PPi-dependent phosphofructokinase (PMID:35104280). Genetic deletion of PFK1 does not impair parasite proliferation in vitro or virulence in vivo, establishing that PFK1 is dispensable for growth (PMID:35104280). Beyond its cytoplasmic localization (PMID:35104280) and dispensability (PMID:35104280), no further mechanistic detail on PFK1 has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 2022 Medium

    It was unknown whether the ATP-dependent phosphofructokinase contributes non-redundantly to Toxoplasma glycolysis and fitness; targeted deletion showed PFK1 is dispensable for both proliferation and virulence, establishing that ATP-PFK activity is not required for parasite growth.

    Evidence Gene deletion / knockout with in vitro proliferation and in vivo virulence assays in Toxoplasma gondii

    PMID:35104280

    Open questions at the time
    • Does not test functional redundancy with the PPi-dependent phosphofructokinase (e.g. double-deletion or conditional depletion)
    • No direct enzymatic characterization of PFK1 activity
    • No metabolic flux measurement to confirm glycolytic contribution
  2. 2022 Low

    The subcellular compartment of PFK1 was undefined; localization studies placed PFK1 in the cytoplasm, consistent with a role in cytosolic glycolysis.

    Evidence Subcellular localization experiment (fractionation / imaging) in Toxoplasma gondii

    PMID:35104280

    Open questions at the time
    • Localization reported without detailed methodology specific to PFK1
    • No co-localization or high-resolution imaging to exclude additional compartments

Mechanism profile

Synthesis pass · controlled-vocabulary classification
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2022 Toxoplasma gondii expresses both ATP-dependent (ATP-PFK, i.e. PFK1) and PPi-dependent phosphofructokinases (PPi-PFK) in the cytoplasm; deletion of ATP-PFK (PFK1) did not affect parasite proliferation or virulence, demonstrating it is dispensable for growth. Gene deletion / knockout with parasite growth and virulence assays PLoS Pathogens Medium 35104280
2022 PFK1 (ATP-PFK) localizes to the cytoplasm of Toxoplasma gondii, as determined by localization studies showing both phosphofructokinases are cytoplasmic. Subcellular localization experiment (fractionation / imaging) PLoS Pathogens Low 35104280

Source papers

Stage 0 corpus · 1 paper · ranked by NIH iCite citations
Year Title Journal Citations PMID
2022 Essential role of pyrophosphate homeostasis mediated by the pyrophosphate-dependent phosphofructokinase in Toxoplasma gondii. PLoS pathogens 18 35104280