{"gene":"TG_316340","gene_symbol":"IMC22","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"IMC22 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with known IMC proteins as bait, placing IMC22 within the IMC organelle composed of alveolar sacs and an underlying cytoskeletal network.","method":"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry identification","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 3 / Weak — single proximity-labeling screen identifying IMC22 as an IMC component; no functional knockout or orthogonal localization validation reported specifically for IMC22 in this abstract","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC22 is a novel inner membrane complex protein of Toxoplasma gondii identified by BioID proximity labeling, localizing to the IMC alveolar/cytoskeletal compartment, but its specific molecular function remains uncharacterized."},"narrative":{"mechanistic_narrative":"IMC22 is a component of the inner membrane complex (IMC) of Toxoplasma gondii, an organelle composed of alveolar sacs and an underlying cytoskeletal network, identified through proximity-dependent biotin identification (BioID) using known IMC proteins as bait [PMID:25691595]. Beyond this proximity-based assignment to the IMC compartment, nothing further about the molecular function of IMC22 has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"The composition of the Toxoplasma IMC was incompletely defined; BioID proximity labeling with known IMC baits established IMC22 as a previously unrecognized resident of this organelle.","evidence":"BioID proximity-dependent biotin identification with IMC-protein baits followed by mass spectrometry in T. gondii","pmids":["25691595"],"confidence":"Medium","gaps":["No functional knockout or knockdown of IMC22 was performed","No orthogonal localization validation specific to IMC22 was reported","Whether IMC22 associates with the alveolar sac membranes or the cytoskeletal network was not resolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Inner membrane complex (IMC)"],"partners":[],"other_free_text":["Identified by BioID proximity labeling with known IMC proteins as bait; associated with the alveolar sac / cytoskeletal compartment of the IMC."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3086,"output_tokens":359,"usd":0.007321,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4949,"output_tokens":894,"usd":0.023547,"stage2_stop_reason":"end_turn"},"total_usd":0.032311,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"IMC22 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with known IMC proteins as bait, placing IMC22 within the IMC organelle composed of alveolar sacs and an underlying cytoskeletal network.\",\n      \"method\": \"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry identification\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single proximity-labeling screen identifying IMC22 as an IMC component; no functional knockout or orthogonal localization validation reported specifically for IMC22 in this abstract\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC22 is a novel inner membrane complex protein of Toxoplasma gondii identified by BioID proximity labeling, localizing to the IMC alveolar/cytoskeletal compartment, but its specific molecular function remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC22 is a component of the inner membrane complex (IMC) of Toxoplasma gondii, an organelle composed of alveolar sacs and an underlying cytoskeletal network, identified through proximity-dependent biotin identification (BioID) using known IMC proteins as bait [#0]. Beyond this proximity-based assignment to the IMC compartment, nothing further about the molecular function of IMC22 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"The composition of the Toxoplasma IMC was incompletely defined; BioID proximity labeling with known IMC baits established IMC22 as a previously unrecognized resident of this organelle.\",\n      \"evidence\": \"BioID proximity-dependent biotin identification with IMC-protein baits followed by mass spectrometry in T. gondii\",\n      \"pmids\": [\"25691595\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional knockout or knockdown of IMC22 was performed\",\n        \"No orthogonal localization validation specific to IMC22 was reported\",\n        \"Whether IMC22 associates with the alveolar sac membranes or the cytoskeletal network was not resolved\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular activity, binding partners, and cellular role of IMC22 within the IMC remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No defined biochemical or structural function for IMC22\",\n        \"No characterized direct interaction partners\",\n        \"No loss-of-function phenotype established\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"inner membrane complex\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"Inner membrane complex (IMC)\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified by BioID proximity labeling with known IMC proteins as bait; associated with the alveolar sac / cytoskeletal compartment of the IMC.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_316340","name":"IMC22","product":"inner membrane complex protein IMC22","uniprot_acc":"A0A125YHU0","chromosome":"TGME49_chrXI","start_pos":5247249},{"strain":"GT1","gene_id":"TGGT1_316340","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrXI","start_pos":5191484},{"strain":"RH88","gene_id":"TGRH88_055080","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023092","start_pos":5313464},{"strain":"VEG","gene_id":"TGVEG_316340","name":null,"product":"hypothetical protein","uniprot_acc":"B6K9S7","chromosome":"TGVEG_chrXI","start_pos":5269879}]}