{"gene":"TG_314810","gene_symbol":"RPL7","run_date":"2026-09-08T15:15:13","timeline":{"discoveries":[{"year":2022,"finding":"TgRPL7 (ribosomal protein RPL7) was localized to the membrane surface of Toxoplasma gondii tachyzoites, as determined by immunofluorescence assay, and was detected as a circulating antigen in the serum of infected hosts during acute infection.","method":"Immunofluorescence assay for subcellular localization; immunoprecipitation-shotgun proteomics and Western blotting for detection in serum","journal":"Journal of Immunology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, single immunofluorescence method for localization with no functional consequence tested; localization finding is incidental to a diagnostic study","pmids":["35022274"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TgRPL7 is a ribosomal protein of Toxoplasma gondii that localizes to the membrane surface and is secreted/shed into the host serum during acute infection, but no further mechanistic details about its function have been established in the available literature."},"narrative":{"mechanistic_narrative":"TgRPL7 is annotated as a ribosomal protein of Toxoplasma gondii that, beyond its expected ribosomal identity, was found associated with the tachyzoite membrane surface and detected as a circulating antigen in host serum during acute infection [PMID:35022274]. Beyond this single localization and serum-detection observation, no functional, mechanistic, or interaction detail for TgRPL7 has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"It was unknown whether TgRPL7 had any presentation outside the ribosome; immunolocalization and serum proteomics showed it at the tachyzoite membrane surface and as a circulating antigen, establishing it as a host-exposed, potentially diagnostic marker during acute infection.","evidence":"Immunofluorescence for subcellular localization plus immunoprecipitation-shotgun proteomics and Western blotting on infected-host serum","pmids":["35022274"],"confidence":"Low","gaps":["Single lab, single immunofluorescence method for localization with no functional consequence tested; incidental to a diagnostic study","No mechanism explaining how a ribosomal protein reaches the membrane surface or is secreted/shed","No functional role, interaction partner, or contribution to infection established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"How TgRPL7 is trafficked to the membrane surface and released into serum, and whether it has any non-ribosomal function during infection, remains unknown.","evidence":"","pmids":[],"confidence":"Low","gaps":["No secretion or trafficking mechanism identified","No functional characterization of a non-ribosomal role","No interaction partners or host targets defined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":["Detected as a circulating antigen in host serum during acute Toxoplasma gondii infection [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"35022274","id":"PMC_35022274","title":"Diagnostic Value of Circulating Antigens in the Serum of Piglets with Experimental Acute Toxoplasmosis.","date":"2022","source":"Journal of immunology (Baltimore, Md. : 1950)","url":"https://pubmed.ncbi.nlm.nih.gov/35022274","citation_count":1,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3024,"output_tokens":339,"usd":0.007078,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4899,"output_tokens":954,"usd":0.024172,"stage2_stop_reason":"end_turn"},"total_usd":0.03125,"stage1_batch_id":"msgbatch_01S6GjoYQm8LTPUsdhASdqag","stage2_batch_id":"msgbatch_01VX63RTMdBJqsG6gQMqoCjz","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"TgRPL7 (ribosomal protein RPL7) was localized to the membrane surface of Toxoplasma gondii tachyzoites, as determined by immunofluorescence assay, and was detected as a circulating antigen in the serum of infected hosts during acute infection.\",\n      \"method\": \"Immunofluorescence assay for subcellular localization; immunoprecipitation-shotgun proteomics and Western blotting for detection in serum\",\n      \"journal\": \"Journal of Immunology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, single immunofluorescence method for localization with no functional consequence tested; localization finding is incidental to a diagnostic study\",\n      \"pmids\": [\"35022274\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"TgRPL7 is a ribosomal protein of Toxoplasma gondii that localizes to the membrane surface and is secreted/shed into the host serum during acute infection, but no further mechanistic details about its function have been established in the available literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"TgRPL7 is annotated as a ribosomal protein of Toxoplasma gondii that, beyond its expected ribosomal identity, was found associated with the tachyzoite membrane surface and detected as a circulating antigen in host serum during acute infection [#0]. Beyond this single localization and serum-detection observation, no functional, mechanistic, or interaction detail for TgRPL7 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"It was unknown whether TgRPL7 had any presentation outside the ribosome; immunolocalization and serum proteomics showed it at the tachyzoite membrane surface and as a circulating antigen, establishing it as a host-exposed, potentially diagnostic marker during acute infection.\",\n      \"evidence\": \"Immunofluorescence for subcellular localization plus immunoprecipitation-shotgun proteomics and Western blotting on infected-host serum\",\n      \"pmids\": [\n        \"35022274\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single lab, single immunofluorescence method for localization with no functional consequence tested; incidental to a diagnostic study\",\n        \"No mechanism explaining how a ribosomal protein reaches the membrane surface or is secreted/shed\",\n        \"No functional role, interaction partner, or contribution to infection established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How TgRPL7 is trafficked to the membrane surface and released into serum, and whether it has any non-ribosomal function during infection, remains unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No secretion or trafficking mechanism identified\",\n        \"No functional characterization of a non-ribosomal role\",\n        \"No interaction partners or host targets defined\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0009986\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Detected as a circulating antigen in host serum during acute Toxoplasma gondii infection [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_314810","name":"RPL7","product":"ribosomal protein RPL7","uniprot_acc":"A0A125YUF7","chromosome":"TGME49_chrXI","start_pos":4309773},{"strain":"GT1","gene_id":"TGGT1_314810","name":null,"product":"ribosomal protein RPL7","uniprot_acc":null,"chromosome":"TGGT1_chrXI","start_pos":4256933},{"strain":"RH88","gene_id":"TGRH88_053800","name":null,"product":"ribosomal protein RPL7","uniprot_acc":null,"chromosome":"CM023092","start_pos":4378909},{"strain":"VEG","gene_id":"TGVEG_314810","name":null,"product":"ribosomal protein RPL7","uniprot_acc":"B9PIY5","chromosome":"TGVEG_chrXI","start_pos":4332287}]}