{"gene":"TG_313550","gene_symbol":"RPL22","run_date":"2026-09-08T15:15:13","timeline":{"discoveries":[{"year":2010,"finding":"RPL22 protein levels in T. gondii decrease upon conditional knockdown of the ribosomal small subunit protein RPS13, but at a slower rate than RPS13 itself, indicating that RPL22 expression is coupled to ribosomal biogenesis and RPS13-dependent translational activity.","method":"Conditional knockdown of rps13 using TetR/ATc system with Myc-tagged RPL22 expression and protein quantitation","journal":"PloS one","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — single lab, direct protein quantitation by tagged construct, but RPL22 is a secondary observation in an RPS13-focused study","pmids":["21124925"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"T. gondii RPL22 is a large ribosomal subunit protein whose expression is coupled to ribosomal biogenesis, as its protein levels decline (albeit more slowly) when the small subunit protein RPS13 is depleted, linking RPL22 to ribosome assembly and parasite proliferation."},"narrative":{"mechanistic_narrative":"In Toxoplasma gondii, RPL22 is a large ribosomal subunit protein whose expression is coupled to ribosome biogenesis: conditional knockdown of the small subunit protein RPS13 causes RPL22 protein levels to decline, though more slowly than RPS13 itself, linking RPL22 abundance to RPS13-dependent translational activity and ribosome assembly [PMID:21124925]. Beyond this coupling observation [PMID:21124925], no further mechanistic detail on RPL22 has been characterized in the available corpus.","teleology":[{"year":2010,"claim":"It was unknown whether RPL22 abundance in T. gondii is tied to overall ribosome biogenesis; depleting the small subunit protein RPS13 showed RPL22 levels fall in parallel but more slowly, establishing that RPL22 expression is coupled to RPS13-dependent ribosomal biogenesis.","evidence":"Conditional TetR/ATc knockdown of rps13 with Myc-tagged RPL22 expression and protein quantitation in T. gondii","pmids":["21124925"],"confidence":"Medium","gaps":["RPL22 was a secondary observation in an RPS13-focused study; its own function was not directly interrogated","No direct knockdown or knockout of RPL22 to define its role in ribosome assembly or parasite proliferation","No structural, localization, or binding-partner data for RPL22"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The direct molecular function of RPL22, its position within the large ribosomal subunit, and any extra-ribosomal roles remain uncharacterized in the available corpus.","evidence":"","pmids":[],"confidence":"Low","gaps":["No direct loss-of-function study of RPL22 itself","No structural or interaction data","No evidence for functions beyond coupling to ribosome biogenesis"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Large ribosomal subunit"],"partners":[],"other_free_text":["RPL22 protein abundance is coupled to RPS13-dependent ribosome biogenesis, declining upon RPS13 knockdown but more slowly than RPS13 [#0]."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"21124925","id":"PMC_21124925","title":"T. gondii RP promoters & knockdown reveal molecular pathways associated with proliferation and cell-cycle arrest.","date":"2010","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/21124925","citation_count":22,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3138,"output_tokens":343,"usd":0.00728,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4929,"output_tokens":1080,"usd":0.025823,"stage2_stop_reason":"end_turn"},"total_usd":0.033103,"stage1_batch_id":"msgbatch_01S6GjoYQm8LTPUsdhASdqag","stage2_batch_id":"msgbatch_01VX63RTMdBJqsG6gQMqoCjz","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2010,\n      \"finding\": \"RPL22 protein levels in T. gondii decrease upon conditional knockdown of the ribosomal small subunit protein RPS13, but at a slower rate than RPS13 itself, indicating that RPL22 expression is coupled to ribosomal biogenesis and RPS13-dependent translational activity.\",\n      \"method\": \"Conditional knockdown of rps13 using TetR/ATc system with Myc-tagged RPL22 expression and protein quantitation\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — single lab, direct protein quantitation by tagged construct, but RPL22 is a secondary observation in an RPS13-focused study\",\n      \"pmids\": [\"21124925\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"T. gondii RPL22 is a large ribosomal subunit protein whose expression is coupled to ribosomal biogenesis, as its protein levels decline (albeit more slowly) when the small subunit protein RPS13 is depleted, linking RPL22 to ribosome assembly and parasite proliferation.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"In Toxoplasma gondii, RPL22 is a large ribosomal subunit protein whose expression is coupled to ribosome biogenesis: conditional knockdown of the small subunit protein RPS13 causes RPL22 protein levels to decline, though more slowly than RPS13 itself, linking RPL22 abundance to RPS13-dependent translational activity and ribosome assembly [#0]. Beyond this coupling observation [#0], no further mechanistic detail on RPL22 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2010,\n      \"claim\": \"It was unknown whether RPL22 abundance in T. gondii is tied to overall ribosome biogenesis; depleting the small subunit protein RPS13 showed RPL22 levels fall in parallel but more slowly, establishing that RPL22 expression is coupled to RPS13-dependent ribosomal biogenesis.\",\n      \"evidence\": \"Conditional TetR/ATc knockdown of rps13 with Myc-tagged RPL22 expression and protein quantitation in T. gondii\",\n      \"pmids\": [\"21124925\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"RPL22 was a secondary observation in an RPS13-focused study; its own function was not directly interrogated\",\n        \"No direct knockdown or knockout of RPL22 to define its role in ribosome assembly or parasite proliferation\",\n        \"No structural, localization, or binding-partner data for RPL22\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct molecular function of RPL22, its position within the large ribosomal subunit, and any extra-ribosomal roles remain uncharacterized in the available corpus.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No direct loss-of-function study of RPL22 itself\",\n        \"No structural or interaction data\",\n        \"No evidence for functions beyond coupling to ribosome biogenesis\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003735\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [],\n    \"pathway\": [\n      {\"term_id\": \"GO:0042254\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [\"Large ribosomal subunit\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"RPL22 protein abundance is coupled to RPS13-dependent ribosome biogenesis, declining upon RPS13 knockdown but more slowly than RPS13 [#0].\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_313550","name":"RPL22","product":"ribosomal protein RPL22","uniprot_acc":"S8ERD4","chromosome":"TGME49_chrXI","start_pos":3469648},{"strain":"GT1","gene_id":"TGGT1_313550","name":null,"product":"ribosomal protein RPL22","uniprot_acc":null,"chromosome":"TGGT1_chrXI","start_pos":3433952},{"strain":"RH88","gene_id":"TGRH88_052700","name":null,"product":"ribosomal protein RPL22","uniprot_acc":null,"chromosome":"CM023092","start_pos":3519022},{"strain":"VEG","gene_id":"TGVEG_313550","name":null,"product":"ribosomal protein RPL22","uniprot_acc":"A0A125YG96","chromosome":"TGVEG_chrXI","start_pos":3483324}]}