{"gene":"TG_304670","gene_symbol":"IMC23","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"IMC23 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) by BioID proximity-dependent biotinylation using known IMC proteins as bait, placing it within the IMC organelle consisting of alveolar sacs and an underlying cytoskeletal network.","method":"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — BioID is a proximity-labeling approach (not direct interaction), single lab; IMC23 was identified but not individually functionally validated by knockout in this study","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC23 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling, localizing to the IMC alveolar/cytoskeletal compartment, though its precise molecular function within the IMC has not yet been experimentally defined."},"narrative":{"mechanistic_narrative":"IMC23 is a component of the Toxoplasma gondii inner membrane complex (IMC), an organelle composed of alveolar sacs overlying a cytoskeletal network, identified by BioID proximity-dependent biotinylation using known IMC proteins as bait [PMID:25691595]. Beyond this proximity-based assignment to the IMC compartment [PMID:25691595], no further mechanistic detail about IMC23's molecular function has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"Before this work IMC23 was uncharacterized; proximity labeling with known IMC baits established that IMC23 resides within the IMC organelle, placing it among the parasite's alveolar/cytoskeletal machinery.","evidence":"BioID proximity-dependent biotinylation with IMC protein baits followed by mass spectrometry in T. gondii","pmids":["25691595"],"confidence":"Medium","gaps":["BioID reports proximity, not direct interaction, so IMC23's binding partners within the IMC are undefined","IMC23 was not functionally validated by knockout, so its role in the IMC is unknown","Sub-compartment localization (alveolar membrane vs cytoskeletal network) was not resolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii inner membrane complex (IMC)"],"partners":[],"other_free_text":["Assigned to the IMC by BioID proximity labeling; not individually functionally validated by knockout."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3086,"output_tokens":339,"usd":0.007171,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4916,"output_tokens":922,"usd":0.023815,"stage2_stop_reason":"end_turn"},"total_usd":0.033385,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"IMC23 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) by BioID proximity-dependent biotinylation using known IMC proteins as bait, placing it within the IMC organelle consisting of alveolar sacs and an underlying cytoskeletal network.\",\n      \"method\": \"BioID (proximity-dependent biotin identification) in T. gondii, followed by mass spectrometry\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — BioID is a proximity-labeling approach (not direct interaction), single lab; IMC23 was identified but not individually functionally validated by knockout in this study\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC23 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling, localizing to the IMC alveolar/cytoskeletal compartment, though its precise molecular function within the IMC has not yet been experimentally defined.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC23 is a component of the Toxoplasma gondii inner membrane complex (IMC), an organelle composed of alveolar sacs overlying a cytoskeletal network, identified by BioID proximity-dependent biotinylation using known IMC proteins as bait [#0]. Beyond this proximity-based assignment to the IMC compartment [#0], no further mechanistic detail about IMC23's molecular function has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"Before this work IMC23 was uncharacterized; proximity labeling with known IMC baits established that IMC23 resides within the IMC organelle, placing it among the parasite's alveolar/cytoskeletal machinery.\",\n      \"evidence\": \"BioID proximity-dependent biotinylation with IMC protein baits followed by mass spectrometry in T. gondii\",\n      \"pmids\": [\n        \"25691595\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"BioID reports proximity, not direct interaction, so IMC23's binding partners within the IMC are undefined\",\n        \"IMC23 was not functionally validated by knockout, so its role in the IMC is unknown\",\n        \"Sub-compartment localization (alveolar membrane vs cytoskeletal network) was not resolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of IMC23 within the IMC, its direct interaction partners, and the phenotypic consequences of its loss remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No knockout or knockdown phenotype established\",\n        \"No direct binding partner or structural role defined\",\n        \"Contribution of the leucine-rich-repeat feature to IMC function untested\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"inner membrane complex\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Toxoplasma gondii inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Assigned to the IMC by BioID proximity labeling; not individually functionally validated by knockout.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_304670","name":"IMC23","product":"inner membrane complex protein IMC23","uniprot_acc":"A0A125YYU3","chromosome":"TGME49_chrVIIa","start_pos":615099},{"strain":"GT1","gene_id":"TGGT1_304670","name":null,"product":"leucine rich repeat-containing protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIIa","start_pos":590918},{"strain":"RH88","gene_id":"TGRH88_033190","name":null,"product":"leucine rich repeat-containing protein","uniprot_acc":null,"chromosome":"CM023088","start_pos":695256},{"strain":"VEG","gene_id":"TGVEG_304670","name":null,"product":"leucine rich repeat-containing protein","uniprot_acc":"B9QR23","chromosome":"TGVEG_chrVIIa","start_pos":672337}]}