{"gene":"TG_300290","gene_symbol":"STX10","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2021,"finding":"TgStx10 localizes to the endosome-like compartment (ELC) in Toxoplasma gondii, as determined by fluorescence localization studies of SNAREs across intracellular compartments.","method":"Fluorescence localization / subcellular fractionation of SNARE proteins","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — direct localization experiment in a systematic SNARE dissection study, single lab, single primary method for this specific finding","pmids":["34340555"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2021,"finding":"TgStx10 resides at the endosome-like compartment alongside TgStx12, placing it in the endosomal vesicular trafficking pathway in T. gondii.","method":"Conditional ablation / localization analysis of SNARE proteins","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — systematic SNARE localization study with conditional knockouts of related SNAREs; TgStx10's specific functional role not independently tested by knockout in this paper","pmids":["34340555"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TgStx10 (STX10) is a SNARE protein that localizes to the endosome-like compartment in Toxoplasma gondii, placing it in the endosomal vesicular trafficking pathway, though its specific functional role within that compartment has not yet been resolved by loss-of-function experiments."},"narrative":{"mechanistic_narrative":"STX10 (TgStx10) is a SNARE protein assigned to the endosomal vesicular trafficking pathway in Toxoplasma gondii, where it localizes to the endosome-like compartment (ELC) [PMID:34340555]. It resides at this compartment alongside TgStx12, consistent with a role in membrane trafficking through the endosomal system [PMID:34340555]. Beyond this localization, the specific functional role of TgStx10 has not been resolved by loss-of-function experiments in the available corpus.","teleology":[{"year":2021,"claim":"To place an uncharacterized SNARE within the parasite's trafficking machinery, fluorescence localization mapped TgStx10 to the endosome-like compartment, establishing which membrane system it operates in.","evidence":"Fluorescence localization and subcellular fractionation of SNAREs across intracellular compartments in T. gondii","pmids":["34340555"],"confidence":"Medium","gaps":["No loss-of-function test of TgStx10's specific role at the ELC","SNARE partners of TgStx10 not identified","No functional trafficking cargo assigned"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2021,"claim":"To situate TgStx10 relative to other endosomal SNAREs, co-localization placed it at the ELC alongside TgStx12, embedding it in the endosomal vesicular trafficking pathway.","evidence":"Conditional ablation and localization analysis of a panel of T. gondii SNARE proteins","pmids":["34340555"],"confidence":"Medium","gaps":["TgStx10's own function not tested by conditional knockout in this study","Whether TgStx10 and TgStx12 form a functional SNARE complex is not established","Directionality of trafficking (in vs out of the ELC) unresolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The specific functional role of TgStx10 within the endosome-like compartment and its cognate SNARE-complex partners remain undefined.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No loss-of-function phenotype for TgStx10","SNARE-complex partners and fusion targets unidentified","Contribution to parasite viability or trafficking flux unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["TGSTX12 (CO-RESIDENT AT THE ENDOSOME-LIKE COMPARTMENT)"],"other_free_text":["TgStx10 localizes to the endosome-like compartment (ELC) in Toxoplasma gondii and is placed in the endosomal vesicular trafficking pathway; its specific function has not been tested by loss-of-function experiments in the available corpus."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"22153078","id":"PMC_22153078","title":"Sec22b regulates phagosomal maturation and antigen crosspresentation by dendritic cells.","date":"2011","source":"Cell","url":"https://pubmed.ncbi.nlm.nih.gov/22153078","citation_count":253,"is_preprint":false},{"pmid":"34340555","id":"PMC_34340555","title":"The Dissection of SNAREs Reveals Key Factors for Vesicular Trafficking to the Endosome-like Compartment and Apicoplast via the Secretory System in Toxoplasma gondii.","date":"2021","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/34340555","citation_count":20,"is_preprint":false},{"pmid":"38270431","id":"PMC_38270431","title":"Apical annuli are specialised sites of post-invasion secretion of dense granules in Toxoplasma.","date":"2024","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/38270431","citation_count":10,"is_preprint":false},{"pmid":"26566590","id":"PMC_26566590","title":"Phosphorylation of αSNAP is Required for Secretory Organelle Biogenesis in Toxoplasma gondii.","date":"2015","source":"Traffic (Copenhagen, Denmark)","url":"https://pubmed.ncbi.nlm.nih.gov/26566590","citation_count":8,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":7639,"output_tokens":501,"usd":0.015216,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5148,"output_tokens":1353,"usd":0.029782,"stage2_stop_reason":"end_turn"},"total_usd":0.044998,"stage1_batch_id":"msgbatch_01S6GjoYQm8LTPUsdhASdqag","stage2_batch_id":"msgbatch_01VX63RTMdBJqsG6gQMqoCjz","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"paper list: removed papers about other genes"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2021,\n      \"finding\": \"TgStx10 localizes to the endosome-like compartment (ELC) in Toxoplasma gondii, as determined by fluorescence localization studies of SNAREs across intracellular compartments.\",\n      \"method\": \"Fluorescence localization / subcellular fractionation of SNARE proteins\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — direct localization experiment in a systematic SNARE dissection study, single lab, single primary method for this specific finding\",\n      \"pmids\": [\"34340555\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2021,\n      \"finding\": \"TgStx10 resides at the endosome-like compartment alongside TgStx12, placing it in the endosomal vesicular trafficking pathway in T. gondii.\",\n      \"method\": \"Conditional ablation / localization analysis of SNARE proteins\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — systematic SNARE localization study with conditional knockouts of related SNAREs; TgStx10's specific functional role not independently tested by knockout in this paper\",\n      \"pmids\": [\"34340555\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"TgStx10 (STX10) is a SNARE protein that localizes to the endosome-like compartment in Toxoplasma gondii, placing it in the endosomal vesicular trafficking pathway, though its specific functional role within that compartment has not yet been resolved by loss-of-function experiments.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"STX10 (TgStx10) is a SNARE protein assigned to the endosomal vesicular trafficking pathway in Toxoplasma gondii, where it localizes to the endosome-like compartment (ELC) [#0]. It resides at this compartment alongside TgStx12, consistent with a role in membrane trafficking through the endosomal system [#1]. Beyond this localization, the specific functional role of TgStx10 has not been resolved by loss-of-function experiments in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"To place an uncharacterized SNARE within the parasite's trafficking machinery, fluorescence localization mapped TgStx10 to the endosome-like compartment, establishing which membrane system it operates in.\",\n      \"evidence\": \"Fluorescence localization and subcellular fractionation of SNAREs across intracellular compartments in T. gondii\",\n      \"pmids\": [\"34340555\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No loss-of-function test of TgStx10's specific role at the ELC\",\n        \"SNARE partners of TgStx10 not identified\",\n        \"No functional trafficking cargo assigned\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2021,\n      \"claim\": \"To situate TgStx10 relative to other endosomal SNAREs, co-localization placed it at the ELC alongside TgStx12, embedding it in the endosomal vesicular trafficking pathway.\",\n      \"evidence\": \"Conditional ablation and localization analysis of a panel of T. gondii SNARE proteins\",\n      \"pmids\": [\"34340555\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"TgStx10's own function not tested by conditional knockout in this study\",\n        \"Whether TgStx10 and TgStx12 form a functional SNARE complex is not established\",\n        \"Directionality of trafficking (in vs out of the ELC) unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific functional role of TgStx10 within the endosome-like compartment and its cognate SNARE-complex partners remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No loss-of-function phenotype for TgStx10\",\n        \"SNARE-complex partners and fusion targets unidentified\",\n        \"Contribution to parasite viability or trafficking flux unknown\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0005484\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005768\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0016192\", \"supporting_discovery_ids\": [1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\"TgStx12 (co-resident at the endosome-like compartment)\"],\n    \"other_free_text\": [\n      \"TgStx10 localizes to the endosome-like compartment (ELC) in Toxoplasma gondii and is placed in the endosomal vesicular trafficking pathway; its specific function has not been tested by loss-of-function experiments in the available corpus.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_300290","name":"STX10","product":"syntaxin-10","uniprot_acc":"S8GAU7","chromosome":"TGME49_chrXII","start_pos":105714},{"strain":"GT1","gene_id":"TGGT1_300290","name":null,"product":"SNARE domain-containing protein","uniprot_acc":null,"chromosome":"TGGT1_chrXII","start_pos":67383},{"strain":"RH88","gene_id":"TGRH88_057090","name":null,"product":"SNARE domain-containing protein","uniprot_acc":null,"chromosome":"CM023093","start_pos":96699},{"strain":"VEG","gene_id":"TGVEG_300290","name":null,"product":"SNARE domain-containing protein","uniprot_acc":"B9QQA2","chromosome":"TGVEG_chrXII","start_pos":96881}]}