{"gene":"TG_297870","gene_symbol":"IMC36","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2023,"finding":"IMC36 was identified as one of eight novel IMC proteins enriched in daughter buds, revealed by proximity labeling from IMC29, significantly expanding the daughter IMC proteome.","method":"Proximity labeling (BioID) from IMC29 in Toxoplasma gondii","journal":"mBio","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single proximity-labeling screen identifying IMC36 as a candidate daughter-enriched IMC protein; no direct functional or mechanistic characterization of IMC36 itself reported","pmids":["36622147"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC36 is a novel daughter bud-enriched inner membrane complex protein identified by proximity labeling, but its specific molecular function and mechanism of action remain uncharacterized."},"narrative":{"mechanistic_narrative":"IMC36 is a novel inner membrane complex protein of Toxoplasma gondii identified as one of eight IMC proteins enriched in daughter buds through proximity labeling from IMC29, an assignment that expands the daughter IMC proteome [PMID:36622147]. Beyond this proximity-based localization to the daughter-bud IMC, nothing further about IMC36's molecular function or mechanism has been characterized in the available corpus.","teleology":[{"year":2023,"claim":"The daughter IMC proteome was incompletely defined; identifying new components addresses which proteins populate the developing daughter bud during endodyogeny.","evidence":"Proximity labeling (BioID) from IMC29 in Toxoplasma gondii","pmids":["36622147"],"confidence":"Low","gaps":["Single proximity-labeling screen with no direct functional characterization of IMC36","No knockout, knockdown, or phenotype established for IMC36","Molecular activity and direct binding partners of IMC36 unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Inner membrane complex (IMC)"],"partners":["IMC29 (PROXIMITY-LABELING BAIT)"],"other_free_text":["IMC36 is a daughter bud-enriched IMC protein identified by BioID proximity labeling from IMC29."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"36622147","id":"PMC_36622147","title":"IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome.","date":"2023","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/36622147","citation_count":30,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3105,"output_tokens":295,"usd":0.00687,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4870,"output_tokens":927,"usd":0.023762,"stage2_stop_reason":"end_turn"},"total_usd":0.031524,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC36 was identified as one of eight novel IMC proteins enriched in daughter buds, revealed by proximity labeling from IMC29, significantly expanding the daughter IMC proteome.\",\n      \"method\": \"Proximity labeling (BioID) from IMC29 in Toxoplasma gondii\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single proximity-labeling screen identifying IMC36 as a candidate daughter-enriched IMC protein; no direct functional or mechanistic characterization of IMC36 itself reported\",\n      \"pmids\": [\"36622147\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC36 is a novel daughter bud-enriched inner membrane complex protein identified by proximity labeling, but its specific molecular function and mechanism of action remain uncharacterized.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"IMC36 is a novel inner membrane complex protein of Toxoplasma gondii identified as one of eight IMC proteins enriched in daughter buds through proximity labeling from IMC29, an assignment that expands the daughter IMC proteome [#0]. Beyond this proximity-based localization to the daughter-bud IMC, nothing further about IMC36's molecular function or mechanism has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2023,\n      \"claim\": \"The daughter IMC proteome was incompletely defined; identifying new components addresses which proteins populate the developing daughter bud during endodyogeny.\",\n      \"evidence\": \"Proximity labeling (BioID) from IMC29 in Toxoplasma gondii\",\n      \"pmids\": [\"36622147\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single proximity-labeling screen with no direct functional characterization of IMC36\",\n        \"No knockout, knockdown, or phenotype established for IMC36\",\n        \"Molecular activity and direct binding partners of IMC36 unknown\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function, essentiality, and mechanistic role of IMC36 within the IMC remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No enzymatic or structural activity assigned\",\n        \"No validated interaction partners beyond proximity to IMC29\",\n        \"No loss-of-function phenotype during daughter bud formation\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0070258\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"Inner membrane complex (IMC)\"],\n    \"partners\": [\"IMC29 (proximity-labeling bait)\"],\n    \"other_free_text\": [\n      \"IMC36 is a daughter bud-enriched IMC protein identified by BioID proximity labeling from IMC29.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}\n```","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_297870","name":"IMC36","product":"inner membrane complex protein IMC36","uniprot_acc":"S8FBI0","chromosome":"TGME49_chrII","start_pos":2086374},{"strain":"GT1","gene_id":"TGGT1_297870","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrII","start_pos":2062540},{"strain":"RH88","gene_id":"TGRH88_002650","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023083","start_pos":2261963},{"strain":"VEG","gene_id":"TGVEG_297870","name":null,"product":"hypothetical protein","uniprot_acc":"V4YNR8","chromosome":"TGVEG_chrII","start_pos":2096331}]}