{"gene":"TG_294340","gene_symbol":"TSC6","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2016,"finding":"TSC6 was identified as one of five new proteins that exclusively localize to the transverse sutures of the Toxoplasma IMC, establishing it as a transverse sutures component (TSC); this localization was determined using proximity-dependent biotin identification (BioID) with ISC4 as bait.","method":"Proximity-dependent biotin identification (BioID) followed by localization validation","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID-based identification with localization confirmation, single lab, single study","pmids":["27696623"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TSC6 is a component of the Toxoplasma inner membrane complex (IMC) that localizes exclusively to the transverse sutures, as identified by proximity-dependent biotinylation; no direct functional characterization of TSC6 itself has been reported."},"narrative":{"mechanistic_narrative":"TSC6 is a component of the Toxoplasma gondii inner membrane complex (IMC) that localizes exclusively to the transverse sutures, identified as one of five transverse sutures components through proximity-dependent biotinylation (BioID) using ISC4 as bait [PMID:27696623]. Beyond this localization assignment, no functional characterization of TSC6 has been reported in the available corpus.","teleology":[{"year":2016,"claim":"The composition of the Toxoplasma IMC transverse sutures was incompletely defined; BioID mapping established TSC6 as a protein restricted to this substructure, placing it within the suture proteome.","evidence":"Proximity-dependent biotin identification (BioID) with ISC4 as bait, followed by localization validation, in Toxoplasma gondii","pmids":["27696623"],"confidence":"Medium","gaps":["No molecular function or biochemical activity for TSC6 has been determined","Direct binding partners of TSC6 within the suture are not defined","The phenotypic consequence of TSC6 loss is unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma IMC transverse sutures (TSC) proteome"],"partners":["ISC4 (BIOID BAIT USED TO IDENTIFY TSC6)"],"other_free_text":["TSC6 identified as one of five transverse sutures components via BioID [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"27696623","id":"PMC_27696623","title":"Novel insights into the composition and function of the Toxoplasma IMC sutures.","date":"2016","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/27696623","citation_count":59,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2890,"output_tokens":341,"usd":0.006893,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4930,"output_tokens":1027,"usd":0.025163,"stage2_stop_reason":"end_turn"},"total_usd":0.064314,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2016,\n      \"finding\": \"TSC6 was identified as one of five new proteins that exclusively localize to the transverse sutures of the Toxoplasma IMC, establishing it as a transverse sutures component (TSC); this localization was determined using proximity-dependent biotin identification (BioID) with ISC4 as bait.\",\n      \"method\": \"Proximity-dependent biotin identification (BioID) followed by localization validation\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID-based identification with localization confirmation, single lab, single study\",\n      \"pmids\": [\"27696623\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"TSC6 is a component of the Toxoplasma inner membrane complex (IMC) that localizes exclusively to the transverse sutures, as identified by proximity-dependent biotinylation; no direct functional characterization of TSC6 itself has been reported.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"TSC6 is a component of the Toxoplasma gondii inner membrane complex (IMC) that localizes exclusively to the transverse sutures, identified as one of five transverse sutures components through proximity-dependent biotinylation (BioID) using ISC4 as bait [#0]. Beyond this localization assignment, no functional characterization of TSC6 has been reported in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2016,\n      \"claim\": \"The composition of the Toxoplasma IMC transverse sutures was incompletely defined; BioID mapping established TSC6 as a protein restricted to this substructure, placing it within the suture proteome.\",\n      \"evidence\": \"Proximity-dependent biotin identification (BioID) with ISC4 as bait, followed by localization validation, in Toxoplasma gondii\",\n      \"pmids\": [\n        \"27696623\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No molecular function or biochemical activity for TSC6 has been determined\",\n        \"Direct binding partners of TSC6 within the suture are not defined\",\n        \"The phenotypic consequence of TSC6 loss is unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How TSC6 contributes to IMC suture architecture, cytoskeletal organization, or parasite biology remains unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or knockout studies of TSC6\",\n        \"No structural model or domain analysis\",\n        \"Mechanism of recruitment to transverse sutures unresolved\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"inner membrane complex transverse sutures\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Toxoplasma IMC transverse sutures (TSC) proteome\"\n    ],\n    \"partners\": [\n      \"ISC4 (BioID bait used to identify TSC6)\"\n    ],\n    \"other_free_text\": [\n      \"TSC6 identified as one of five transverse sutures components via BioID [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_294340","name":"TSC6","product":"transverse sutures component TSC6","uniprot_acc":"S8FFI1","chromosome":"TGME49_chrIa","start_pos":810573},{"strain":"GT1","gene_id":"TGGT1_294340","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrIa","start_pos":797278},{"strain":"RH88","gene_id":"TGRH88_019430","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023081","start_pos":817455},{"strain":"VEG","gene_id":"TGVEG_294340","name":null,"product":"putative transmembrane protein","uniprot_acc":"A0A125YPD9","chromosome":"TGVEG_chrIa","start_pos":820896}]}