{"gene":"TG_291020","gene_symbol":"MyoL","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2017,"finding":"TgUNC acts as a chaperone dedicated to the folding, assembly, and function of all Toxoplasma myosins, including MyoL (a class myosin with a motor domain); conditional ablation of TgUNC recapitulates the phenome of the known myosins and uncovers roles in basal complex constriction and synchronized division within the parasitophorous vacuole across the full myosin repertoire.","method":"Conditional ablation (TgUNC knockdown) with phenotypic readouts of motility, invasion, egress, basal complex constriction, and synchronized division","journal":"Nature Communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — MyoL is mentioned only as part of the broader Toxoplasma myosin repertoire (11 myosins total); no experiment specifically addresses MyoL's individual function, localization, or interactions","pmids":["28593938"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"No mechanistic findings specific to MyoL are established in the available literature; it is noted as one of the 11 myosins in Toxoplasma gondii whose folding and assembly depend on the TgUNC chaperone, but no experiment has individually characterized MyoL's substrates, localization, activity, or cellular function."},"narrative":{"mechanistic_narrative":"MyoL is one of the eleven Toxoplasma gondii myosins whose folding, assembly, and function depend on the co-chaperone TgUNC; conditional ablation of TgUNC recapitulates the collective myosin phenome, including defects in basal complex constriction and synchronized intravacuolar division [PMID:28593938]. No experiment has individually characterized MyoL's substrates, localization, motor activity, or specific cellular function in the available corpus.","teleology":[{"year":2017,"claim":"It was unknown whether Toxoplasma myosins share a common maturation pathway; conditional TgUNC ablation showed that TgUNC is a dedicated chaperone required for the folding and assembly of the full myosin repertoire, placing MyoL within a TgUNC-dependent set of motors and linking loss of this pathway to defects in basal complex constriction and synchronized division.","evidence":"Conditional ablation (TgUNC knockdown) with phenotypic readouts of motility, invasion, egress, basal complex constriction, and synchronized division in Toxoplasma gondii","pmids":["28593938"],"confidence":"Low","gaps":["MyoL is addressed only as part of the collective myosin repertoire; no experiment resolves MyoL's individual function","MyoL's subcellular localization has not been determined","MyoL's substrates, binding partners, and motor activity are uncharacterized"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["TGUNC (CHAPERONE REQUIRED FOR FOLDING AND ASSEMBLY OF MYOL, SHOWN AT THE LEVEL OF THE FULL TOXOPLASMA MYOSIN REPERTOIRE) [#0]"],"other_free_text":["MyoL is one of 11 Toxoplasma gondii myosins whose folding, assembly, and function depend on the co-chaperone TgUNC; ablation of TgUNC produces collective myosin defects in basal complex constriction and synchronized division [#0].","No mechanistic finding is specific to MyoL alone; its individual substrates, localization, motor activity, and cellular role are uncharacterized in the available corpus."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"28593938","id":"PMC_28593938","title":"Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii.","date":"2017","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/28593938","citation_count":90,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2815,"output_tokens":778,"usd":0.010058,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5591,"output_tokens":1860,"usd":0.037227,"stage2_stop_reason":"end_turn"},"total_usd":0.082073,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"TgUNC acts as a chaperone dedicated to the folding, assembly, and function of all Toxoplasma myosins, including MyoL (a class myosin with a motor domain); conditional ablation of TgUNC recapitulates the phenome of the known myosins and uncovers roles in basal complex constriction and synchronized division within the parasitophorous vacuole across the full myosin repertoire.\",\n      \"method\": \"Conditional ablation (TgUNC knockdown) with phenotypic readouts of motility, invasion, egress, basal complex constriction, and synchronized division\",\n      \"journal\": \"Nature Communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — MyoL is mentioned only as part of the broader Toxoplasma myosin repertoire (11 myosins total); no experiment specifically addresses MyoL's individual function, localization, or interactions\",\n      \"pmids\": [\"28593938\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"No mechanistic findings specific to MyoL are established in the available literature; it is noted as one of the 11 myosins in Toxoplasma gondii whose folding and assembly depend on the TgUNC chaperone, but no experiment has individually characterized MyoL's substrates, localization, activity, or cellular function.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"MyoL is one of the eleven Toxoplasma gondii myosins whose folding, assembly, and function depend on the co-chaperone TgUNC; conditional ablation of TgUNC recapitulates the collective myosin phenome, including defects in basal complex constriction and synchronized intravacuolar division [#0]. No experiment has individually characterized MyoL's substrates, localization, motor activity, or specific cellular function in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"It was unknown whether Toxoplasma myosins share a common maturation pathway; conditional TgUNC ablation showed that TgUNC is a dedicated chaperone required for the folding and assembly of the full myosin repertoire, placing MyoL within a TgUNC-dependent set of motors and linking loss of this pathway to defects in basal complex constriction and synchronized division.\",\n      \"evidence\": \"Conditional ablation (TgUNC knockdown) with phenotypic readouts of motility, invasion, egress, basal complex constriction, and synchronized division in Toxoplasma gondii\",\n      \"pmids\": [\"28593938\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"MyoL is addressed only as part of the collective myosin repertoire; no experiment resolves MyoL's individual function\",\n        \"MyoL's subcellular localization has not been determined\",\n        \"MyoL's substrates, binding partners, and motor activity are uncharacterized\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The individual function, localization, activity, and interaction partners of MyoL remain unknown, as no experiment has characterized MyoL apart from the broader TgUNC-dependent myosin repertoire.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No MyoL-specific localization data\",\n        \"No MyoL-specific motor activity or substrate/partner identification\",\n        \"No MyoL-specific loss-of-function phenotype\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\"TgUNC (chaperone required for folding and assembly of MyoL, shown at the level of the full Toxoplasma myosin repertoire) [#0]\"],\n    \"other_free_text\": [\n      \"MyoL is one of 11 Toxoplasma gondii myosins whose folding, assembly, and function depend on the co-chaperone TgUNC; ablation of TgUNC produces collective myosin defects in basal complex constriction and synchronized division [#0].\",\n      \"No mechanistic finding is specific to MyoL alone; its individual substrates, localization, motor activity, and cellular role are uncharacterized in the available corpus.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_291020","name":"MyoL","product":"myosin L","uniprot_acc":"S8F3J9","chromosome":"TGME49_chrIX","start_pos":4047255},{"strain":"GT1","gene_id":"TGGT1_291020","name":null,"product":"myosin head (motor domain) domain-containing protein","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":3855898},{"strain":"RH88","gene_id":"TGRH88_015380","name":null,"product":"myosin head (motor domain) domain-containing protein","uniprot_acc":null,"chromosome":"CM023090","start_pos":4111931},{"strain":"VEG","gene_id":"TGVEG_291020","name":null,"product":"myosin head (motor domain) domain-containing protein","uniprot_acc":"V5BEU8","chromosome":"TGVEG_chrIX","start_pos":4082669}]}