{"gene":"TG_288950","gene_symbol":"AP2IX4","run_date":"2026-09-11T11:01:00","timeline":{"discoveries":[{"year":2017,"finding":"AP2IX-4 is a nuclear protein exclusively expressed in dividing tachyzoites and bradyzoites (cell cycle-regulated). Knockout of AP2IX-4 had no discernible effect on tachyzoite replication but resulted in reduced frequency of tissue cyst formation following alkaline stress induction, a defect reversible by complementation. Loss of AP2IX-4 caused dramatic increases in bradyzoite mRNA levels beyond those seen under normal stress induction, indicating AP2IX-4 acts as a transcriptional repressor of bradyzoite genes during parasite differentiation. AP2IX-4 knockout also resulted in modest virulence defect and reduced cyst burden in chronically infected mice, reversed by complementation.","method":"Gene knockout (KO) in tachyzoites, complementation, alkaline stress induction assays, transcriptomic analysis, mouse infection model, immunofluorescence localization","journal":"mSphere","confidence":"High","confidence_rationale":"Tier 2 / Strong — clean KO with defined cellular phenotype, complementation rescue, transcriptomic profiling, in vivo mouse model; multiple orthogonal methods in a single focused study","pmids":["28317026"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2020,"finding":"AP2IX-4 was identified as a component of the MORC transcriptional repressor complex by co-immunoprecipitation and proteomics. AP2IX-4 associates with AP2XII-2 and both show peak expression during S/M phase of the tachyzoite cell cycle. AP2IX-4 is dispensable in tachyzoites (consistent with prior KO studies), while its interacting partner AP2XII-2 is required for proper S-phase progression.","method":"Co-immunoprecipitation, mass spectrometry proteomics, parallel protein expression monitoring by western blot, cell cycle staging","journal":"mSphere","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal Co-IP with MS proteomics identifying MORC complex membership; single lab, two orthogonal methods","pmids":["32938695"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2010,"finding":"AP2IX-4 mRNA exhibits a cyclical expression profile in dividing tachyzoites, with peak expression in the S/M-subtranscriptome (associated with parasite maturation and daughter formation), and the AP2IX-4 protein shows a sharp peak of expression in the same timeframe as its mRNA, suggesting post-transcriptional mechanisms may regulate protein delivery.","method":"Genome-wide cell cycle transcriptome profiling, protein expression timing analysis","journal":"PloS one","confidence":"Low","confidence_rationale":"Tier 4 / Weak — transcriptomic profiling and expression correlation only; no direct functional or mechanistic experiment performed specifically on AP2IX-4","pmids":["20865045"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"AP2IX-4 is a cell cycle-regulated nuclear transcription factor in Toxoplasma gondii, expressed exclusively in dividing tachyzoites and bradyzoites, that directly binds DNA via its AP2 domain and functions as a transcriptional repressor of bradyzoite-specific genes; knockout parasites are viable during tachyzoite replication but show dramatically elevated bradyzoite gene expression under stress conditions, reduced tissue cyst formation in vitro, and reduced brain cyst burden in mice, while biochemical studies place AP2IX-4 within the MORC transcriptional repressor complex and GCN5b-associated chromatin remodeling complexes."},"narrative":{"mechanistic_narrative":"AP2IX-4 is a cell cycle-regulated nuclear AP2-domain transcription factor in Toxoplasma gondii that represses bradyzoite-specific genes during the tachyzoite-to-bradyzoite stage conversion [PMID:28317026]. AP2IX-4 is dispensable for tachyzoite replication, but its loss causes dramatic elevation of bradyzoite transcripts beyond normal stress-induced levels, reduced tissue cyst formation in vitro, and reduced brain cyst burden in chronically infected mice, all reversed by complementation [PMID:28317026]. Biochemically, AP2IX-4 is a component of the MORC transcriptional repressor complex, co-expressed and associated with AP2XII-2 during S/M phase [PMID:32938695]. AP2IX-4 mRNA exhibits a cyclical expression profile in dividing tachyzoites, with peak expression in the S/M-subtranscriptome, and the AP2IX-4 protein shows a sharp peak of expression in the same timeframe as its mRNA, suggesting post-transcriptional mechanisms may regulate protein delivery [PMID:20865045]. The precise genomic targets and the mechanism linking DNA binding to repression have not been characterized in the available corpus.","teleology":[{"year":2010,"claim":"Establishing when AP2IX-4 acts required knowing its expression timing; genome-wide profiling placed both its transcript and protein in a sharp S/M-phase peak, tying it to daughter cell formation and parasite maturation.","evidence":"Genome-wide cell cycle transcriptome profiling with protein expression timing in tachyzoites","pmids":["20865045"],"confidence":"Low","gaps":["Correlative expression only, no functional test of AP2IX-4","No evidence of DNA binding or transcriptional activity","Post-transcriptional regulation inferred but not demonstrated"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2017,"claim":"The direction of AP2IX-4's regulatory role was resolved by showing that its loss dramatically elevates bradyzoite transcripts beyond normal stress induction, establishing it as a transcriptional repressor of bradyzoite genes with in vivo cyst and virulence consequences.","evidence":"Clean knockout with complementation rescue, alkaline stress induction, transcriptomics, and mouse chronic infection model","pmids":["28317026"],"confidence":"High","gaps":["Direct target promoters not identified","Mechanism of repression (chromatin, cofactor recruitment) not defined","Redundancy with other AP2 factors during tachyzoite stage unexplained"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2020,"claim":"The repressive activity was given a biochemical framework by placing AP2IX-4 within the MORC transcriptional repressor complex and pairing it with AP2XII-2 during S/M phase.","evidence":"Reciprocal co-immunoprecipitation with mass spectrometry proteomics and cell cycle-staged western blots","pmids":["32938695"],"confidence":"Medium","gaps":["Functional contribution of AP2IX-4 within the MORC complex not dissected","Whether AP2XII-2 and AP2IX-4 co-target the same genes untested","Structural organization of the complex unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["MORC transcriptional repressor complex"],"partners":["AP2XII-2"],"other_free_text":["Cell cycle-regulated with peak expression in the S/M phase of dividing tachyzoites and bradyzoites [#0, #3].","Dispensable for tachyzoite replication; knockout elevates bradyzoite gene expression and reduces tissue cyst formation and brain cyst burden in mice, reversed by complementation [#0, #1]."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"20865045","id":"PMC_20865045","title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.","date":"2010","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/20865045","citation_count":234,"is_preprint":false},{"pmid":"28317026","id":"PMC_28317026","title":"Toxoplasma gondii AP2IX-4 Regulates Gene Expression during Bradyzoite Development.","date":"2017","source":"mSphere","url":"https://pubmed.ncbi.nlm.nih.gov/28317026","citation_count":51,"is_preprint":false},{"pmid":"29932347","id":"PMC_29932347","title":"The Epigenome, Cell Cycle, and Development in Toxoplasma.","date":"2018","source":"Annual review of microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/29932347","citation_count":51,"is_preprint":false},{"pmid":"20657661","id":"PMC_20657661","title":"The construction and use of log-odds substitution scores for multiple sequence alignment.","date":"2010","source":"PLoS computational biology","url":"https://pubmed.ncbi.nlm.nih.gov/20657661","citation_count":48,"is_preprint":false},{"pmid":"32938695","id":"PMC_32938695","title":"Toxoplasma gondii AP2XII-2 Contributes to Proper Progression through S-Phase of the Cell Cycle.","date":"2020","source":"mSphere","url":"https://pubmed.ncbi.nlm.nih.gov/32938695","citation_count":27,"is_preprint":false},{"pmid":"22486860","id":"PMC_22486860","title":"Cactin is essential for G1 progression in Toxoplasma gondii.","date":"2012","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/22486860","citation_count":23,"is_preprint":false},{"pmid":"25393307","id":"PMC_25393307","title":"Distinct strains of Toxoplasma gondii feature divergent transcriptomes regardless of developmental stage.","date":"2014","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/25393307","citation_count":22,"is_preprint":false},{"pmid":"18983845","id":"PMC_18983845","title":"New eukaryotic systematics: a phylogenetic perspective of developmental gene expression in the Apicomplexa.","date":"2008","source":"International journal for parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/18983845","citation_count":12,"is_preprint":false},{"pmid":"39690155","id":"PMC_39690155","title":"Apicomplexan mitoribosome from highly fragmented rRNAs to a functional machine.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/39690155","citation_count":9,"is_preprint":false},{"pmid":"36178892","id":"PMC_36178892","title":"Genomic insights into host and parasite interactions during intracellular infection by Toxoplasma gondii.","date":"2022","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/36178892","citation_count":2,"is_preprint":false},{"pmid":"40934260","id":"PMC_40934260","title":"HIV-1 Tat favors the multiplication of Mycobacterium tuberculosis and Toxoplasma by inhibiting clathrin-mediated endocytosis and autophagy.","date":"2025","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/40934260","citation_count":0,"is_preprint":false},{"pmid":"42087241","id":"PMC_42087241","title":"Host-cell dependent epigenetic profiles associated with survival outcomes in T. gondii infection.","date":"2026","source":"Epigenetics & chromatin","url":"https://pubmed.ncbi.nlm.nih.gov/42087241","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":19752,"output_tokens":2002,"usd":0.044643,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7380,"output_tokens":2857,"usd":0.054163,"stage2_stop_reason":"end_turn"},"total_usd":0.10304,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"summary amended","hand_review_patch":"2026-09-11 AK hand-review verdicts (handoff/tools_2026-09-11/patch)","hand_review_verdict":"edit"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"AP2IX-4 is a nuclear protein exclusively expressed in dividing tachyzoites and bradyzoites (cell cycle-regulated). Knockout of AP2IX-4 had no discernible effect on tachyzoite replication but resulted in reduced frequency of tissue cyst formation following alkaline stress induction, a defect reversible by complementation. Loss of AP2IX-4 caused dramatic increases in bradyzoite mRNA levels beyond those seen under normal stress induction, indicating AP2IX-4 acts as a transcriptional repressor of bradyzoite genes during parasite differentiation. AP2IX-4 knockout also resulted in modest virulence defect and reduced cyst burden in chronically infected mice, reversed by complementation.\",\n      \"method\": \"Gene knockout (KO) in tachyzoites, complementation, alkaline stress induction assays, transcriptomic analysis, mouse infection model, immunofluorescence localization\",\n      \"journal\": \"mSphere\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — clean KO with defined cellular phenotype, complementation rescue, transcriptomic profiling, in vivo mouse model; multiple orthogonal methods in a single focused study\",\n      \"pmids\": [\"28317026\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"finding\": \"TgAP2XI-4 (AP2IX-4) recombinant AP2 domain specifically binds a DNA motif in gel retardation assays, localizes to the parasite nucleus throughout the tachyzoite life cycle with peak expression after cytokinesis, and its transcript is higher in bradyzoite cysts from chronically infected mice than in tachyzoites. Knockout of TgAP2XI-4 in both type I and type II strains revealed its role in modulating expression and promoter activity of genes involved in tachyzoite-to-bradyzoite stage conversion, and type II KO mutants showed drastically reduced brain cyst burden in mice.\",\n      \"method\": \"Gel retardation assay (EMSA) for DNA binding, gene knockout in type I and type II strains, promoter activity assays, RT-PCR/transcriptomic analysis, mouse infection model, nuclear localization by immunofluorescence\",\n      \"journal\": \"Molecular microbiology\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1–2 / Strong — in vitro DNA-binding assay (EMSA), KO in two strain types, complementary in vivo mouse data, multiple orthogonal methods; independently consistent with PMID:28317026\",\n      \"pmids\": [\"23240624\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"type I\"}, {\"species\": \"Toxoplasma gondii\", \"strain\": \"type II\"}]\n    },\n    {\n      \"year\": 2020,\n      \"finding\": \"AP2IX-4 was identified as a component of the MORC transcriptional repressor complex by co-immunoprecipitation and proteomics. AP2IX-4 associates with AP2XII-2 and both show peak expression during S/M phase of the tachyzoite cell cycle. AP2IX-4 is dispensable in tachyzoites (consistent with prior KO studies), while its interacting partner AP2XII-2 is required for proper S-phase progression.\",\n      \"method\": \"Co-immunoprecipitation, mass spectrometry proteomics, parallel protein expression monitoring by western blot, cell cycle staging\",\n      \"journal\": \"mSphere\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal Co-IP with MS proteomics identifying MORC complex membership; single lab, two orthogonal methods\",\n      \"pmids\": [\"32938695\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2010,\n      \"finding\": \"AP2IX-4 mRNA exhibits a cyclical expression profile in dividing tachyzoites, with peak expression in the S/M-subtranscriptome (associated with parasite maturation and daughter formation), and the AP2IX-4 protein shows a sharp peak of expression in the same timeframe as its mRNA, suggesting post-transcriptional mechanisms may regulate protein delivery.\",\n      \"method\": \"Genome-wide cell cycle transcriptome profiling, protein expression timing analysis\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 4 / Weak — transcriptomic profiling and expression correlation only; no direct functional or mechanistic experiment performed specifically on AP2IX-4\",\n      \"pmids\": [\"20865045\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2014,\n      \"finding\": \"GCN5b, an essential lysine acetyltransferase, was found by proteomics to interact with AP2-domain proteins including AP2IX-4 (inferred from the broader AP2 interactome described). GCN5b forms a complex with AP2 transcription factors, ADA2-A, TFIID subunits, LEO1, and RRM proteins, linking AP2IX-4 to transcriptional and chromatin remodeling complexes.\",\n      \"method\": \"Co-immunoprecipitation, mass spectrometry proteomics (GCN5b pull-down)\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — AP2IX-4 is listed as one of many AP2 proteins identified in the GCN5b interactome; no reciprocal IP or functional validation specific to AP2IX-4 reported\",\n      \"pmids\": [\"24391497\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"GCN5b is associated with at least two distinct complexes characterized by different pairs of AP2 factors; AP2IX-4 was identified as one interacting AP2 factor in the GCN5b complex under normal and bradyzoite induction conditions, consistent with AP2IX-4 participation in GCN5b-mediated epigenetic gene regulation.\",\n      \"method\": \"Co-immunoprecipitation (GCN5b pull-down and reciprocal AP2 factor Co-IPs), mass spectrometry\",\n      \"journal\": \"Molecular and biochemical parasitology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab Co-IP/MS; AP2IX-4 listed among multiple AP2 partners, no specific functional validation of AP2IX-4 within the complex\",\n      \"pmids\": [\"31381949\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"AP2IX-4 is a cell cycle-regulated nuclear transcription factor in Toxoplasma gondii, expressed exclusively in dividing tachyzoites and bradyzoites, that directly binds DNA via its AP2 domain and functions as a transcriptional repressor of bradyzoite-specific genes; knockout parasites are viable during tachyzoite replication but show dramatically elevated bradyzoite gene expression under stress conditions, reduced tissue cyst formation in vitro, and reduced brain cyst burden in mice, while biochemical studies place AP2IX-4 within the MORC transcriptional repressor complex and GCN5b-associated chromatin remodeling complexes.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AP2IX-4 is a cell cycle-regulated nuclear AP2-domain transcription factor in Toxoplasma gondii that represses bradyzoite-specific genes during the tachyzoite-to-bradyzoite stage conversion [#0, #1]. Its recombinant AP2 domain binds a specific DNA motif in vitro, and the protein localizes to the parasite nucleus, with peak expression in dividing tachyzoites and bradyzoites [#1]. AP2IX-4 is dispensable for tachyzoite replication, but its loss causes dramatic elevation of bradyzoite transcripts beyond normal stress-induced levels, reduced tissue cyst formation in vitro, and reduced brain cyst burden in chronically infected mice, all reversed by complementation [#0, #1]. Biochemically, AP2IX-4 is a component of the MORC transcriptional repressor complex, co-expressed and associated with AP2XII-2 during S/M phase [#2]. Beyond these findings, the precise genomic targets and the mechanism linking DNA binding to repression have not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2010,\n      \"claim\": \"Establishing when AP2IX-4 acts required knowing its expression timing; genome-wide profiling placed both its transcript and protein in a sharp S/M-phase peak, tying it to daughter cell formation and parasite maturation.\",\n      \"evidence\": \"Genome-wide cell cycle transcriptome profiling with protein expression timing in tachyzoites\",\n      \"pmids\": [\"20865045\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"Correlative expression only, no functional test of AP2IX-4\", \"No evidence of DNA binding or transcriptional activity\", \"Post-transcriptional regulation inferred but not demonstrated\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2012,\n      \"claim\": \"To move from a timing correlation to a molecular function, the AP2 domain was shown to bind a specific DNA motif in vitro and knockouts in two strain types demonstrated AP2IX-4 modulates stage-conversion gene expression and cyst formation in vivo.\",\n      \"evidence\": \"EMSA DNA-binding assay, knockout in type I and type II strains, promoter activity assays, and mouse infection model\",\n      \"pmids\": [\"23240624\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Genomic in vivo binding sites not mapped\", \"Direct versus indirect regulation of stage-conversion genes not resolved\", \"Mechanism of repression versus activation not defined\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"type I\"}, {\"species\": \"Toxoplasma gondii\", \"strain\": \"type II\"}]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"The direction of AP2IX-4's regulatory role was resolved by showing that its loss dramatically elevates bradyzoite transcripts beyond normal stress induction, establishing it as a transcriptional repressor of bradyzoite genes with in vivo cyst and virulence consequences.\",\n      \"evidence\": \"Clean knockout with complementation rescue, alkaline stress induction, transcriptomics, and mouse chronic infection model\",\n      \"pmids\": [\"28317026\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"Direct target promoters not identified\", \"Mechanism of repression (chromatin, cofactor recruitment) not defined\", \"Redundancy with other AP2 factors during tachyzoite stage unexplained\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2020,\n      \"claim\": \"The repressive activity was given a biochemical framework by placing AP2IX-4 within the MORC transcriptional repressor complex and pairing it with AP2XII-2 during S/M phase.\",\n      \"evidence\": \"Reciprocal co-immunoprecipitation with mass spectrometry proteomics and cell cycle-staged western blots\",\n      \"pmids\": [\"32938695\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"Functional contribution of AP2IX-4 within the MORC complex not dissected\", \"Whether AP2XII-2 and AP2IX-4 co-target the same genes untested\", \"Structural organization of the complex unknown\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"Parallel proteomics linked AP2IX-4 to GCN5b acetyltransferase-associated chromatin remodeling complexes, connecting it to epigenetic gene regulation under normal and bradyzoite-induction conditions.\",\n      \"evidence\": \"GCN5b pull-down and reciprocal AP2-factor co-immunoprecipitation with mass spectrometry\",\n      \"pmids\": [\"24391497\", \"31381949\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"AP2IX-4 listed among many AP2 partners with no AP2IX-4-specific functional validation\", \"No reciprocal IP anchored on AP2IX-4 in the 2014 dataset\", \"Contribution of GCN5b association to bradyzoite gene repression untested\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The genome-wide direct binding sites of AP2IX-4 and the mechanistic link between its DNA binding, MORC/GCN5b complex membership, and repression of specific bradyzoite promoters remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No ChIP-based in vivo target map\", \"Relationship between DNA-motif binding and complex-mediated repression unresolved\", \"Regulation of AP2IX-4 itself during stress not mechanistically established\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003700\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0043565\", \"supporting_discovery_ids\": [1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"type I\"}, {\"species\": \"Toxoplasma gondii\", \"strain\": \"type II\"}]},\n      {\"term_id\": \"GO:0000122\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0030154\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0006338\", \"supporting_discovery_ids\": [4, 5], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [\n      \"MORC transcriptional repressor complex\",\n      \"GCN5b acetyltransferase-associated chromatin remodeling complex\"\n    ],\n    \"partners\": [\n      \"AP2XII-2\",\n      \"GCN5b\",\n      \"ADA2-A\",\n      \"TFIID subunits\",\n      \"LEO1\",\n      \"RRM proteins\"\n    ],\n    \"other_free_text\": [\n      \"Cell cycle-regulated with peak expression in the S/M phase of dividing tachyzoites and bradyzoites [#0, #3].\",\n      \"Dispensable for tachyzoite replication; knockout elevates bradyzoite gene expression and reduces tissue cyst formation and brain cyst burden in mice, reversed by complementation [#0, #1].\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_288950","name":"AP2IX4","product":"AP2 domain transcription factor AP2IX-4","uniprot_acc":"S8G842","chromosome":"TGME49_chrIX","start_pos":2869867},{"strain":"GT1","gene_id":"TGGT1_288950","name":null,"product":"AP2 domain transcription factor AP2IX-4","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":2680268},{"strain":"RH88","gene_id":"TGRH88_013760","name":null,"product":"AP2 domain transcription factor AP2IX-4","uniprot_acc":null,"chromosome":"CM023090","start_pos":2935831},{"strain":"VEG","gene_id":"TGVEG_288950","name":null,"product":"AP2 domain transcription factor AP2IX-4","uniprot_acc":"B9QDW5","chromosome":"TGVEG_chrIX","start_pos":2906396}]}