{"gene":"TG_286580","gene_symbol":"IMC17","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"IMC17 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with known IMC proteins as bait, placing IMC17 within the IMC suborganellar compartment.","method":"BioID (proximity-dependent biotin identification) in T. gondii followed by mass spectrometry; fluorescence localization","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID with localization validation in a single study; IMC17 was identified but not one of the two proteins subjected to functional knockout evaluation","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC17 is a novel protein component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling with established IMC proteins, localizing to the IMC suborganellar compartment, though its specific molecular function within the IMC remains uncharacterized."},"narrative":{"mechanistic_narrative":"IMC17 is a novel protein component of the Toxoplasma gondii inner membrane complex (IMC), identified by proximity-dependent biotin identification (BioID) using established IMC proteins as bait and localized to the IMC suborganellar compartment by fluorescence microscopy [PMID:25691595]. Beyond this identification and localization, nothing further about the molecular function of IMC17 has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"Whether IMC17 was a bona fide constituent of the Toxoplasma IMC was unknown; BioID proximity labeling with known IMC proteins placed IMC17 within the IMC compartment, establishing it as a novel resident of this suborganellar structure.","evidence":"BioID proximity labeling in T. gondii with IMC-protein bait, mass spectrometry, and fluorescence localization","pmids":["25691595"],"confidence":"Medium","gaps":["IMC17 was identified but not subjected to functional knockout evaluation, so its role in the IMC is undefined","No direct binding partners or substrates of IMC17 have been resolved","The submembrane positioning of IMC17 within the IMC (cytoskeletal vs. membrane sublayer) is not established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["inner membrane complex (IMC)"],"partners":[],"other_free_text":["Identified as a novel IMC component by BioID proximity labeling with known IMC proteins as bait [#0]."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3086,"output_tokens":357,"usd":0.007306,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4939,"output_tokens":929,"usd":0.02396,"stage2_stop_reason":"end_turn"},"total_usd":0.033171,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"IMC17 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with known IMC proteins as bait, placing IMC17 within the IMC suborganellar compartment.\",\n      \"method\": \"BioID (proximity-dependent biotin identification) in T. gondii followed by mass spectrometry; fluorescence localization\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID with localization validation in a single study; IMC17 was identified but not one of the two proteins subjected to functional knockout evaluation\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC17 is a novel protein component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling with established IMC proteins, localizing to the IMC suborganellar compartment, though its specific molecular function within the IMC remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC17 is a novel protein component of the Toxoplasma gondii inner membrane complex (IMC), identified by proximity-dependent biotin identification (BioID) using established IMC proteins as bait and localized to the IMC suborganellar compartment by fluorescence microscopy [#0]. Beyond this identification and localization, nothing further about the molecular function of IMC17 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"Whether IMC17 was a bona fide constituent of the Toxoplasma IMC was unknown; BioID proximity labeling with known IMC proteins placed IMC17 within the IMC compartment, establishing it as a novel resident of this suborganellar structure.\",\n      \"evidence\": \"BioID proximity labeling in T. gondii with IMC-protein bait, mass spectrometry, and fluorescence localization\",\n      \"pmids\": [\n        \"25691595\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"IMC17 was identified but not subjected to functional knockout evaluation, so its role in the IMC is undefined\",\n        \"No direct binding partners or substrates of IMC17 have been resolved\",\n        \"The submembrane positioning of IMC17 within the IMC (cytoskeletal vs. membrane sublayer) is not established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of IMC17 within the IMC, its interaction partners, and its contribution to parasite biology remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional assay (knockout, complementation) has been reported for IMC17\",\n        \"No mechanistic role in IMC assembly, motility, or replication has been assigned\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"inner membrane complex\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as a novel IMC component by BioID proximity labeling with known IMC proteins as bait [#0].\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_286580","name":"IMC17","product":"inner membrane complex protein IMC17","uniprot_acc":"S8F5C0","chromosome":"TGME49_chrV","start_pos":1775618},{"strain":"GT1","gene_id":"TGGT1_286580","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrV","start_pos":1631039},{"strain":"RH88","gene_id":"TGRH88_025620","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023086","start_pos":1809126},{"strain":"VEG","gene_id":"TGVEG_286580","name":null,"product":"putative lysophospholipase","uniprot_acc":"B9QG70","chromosome":"TGVEG_chrV","start_pos":1730367}]}