{"gene":"TG_278130","gene_symbol":"BCC11","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"BCC11 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach, placing it within one of four architecturally distinct BC subcomplexes whose composition changes dynamically in correlation with changes in BC function during cell division.","method":"Reciprocal proximity-based biotinylation (BioID) proteomics; spatiotemporal disruption of component expression","journal":"Nature Communications","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — reciprocal BioID proteomics with functional follow-up, but BCC11-specific mechanistic details are not individually reported beyond its inclusion in the BC proteome map","pmids":["35941170"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"BCC11 is a basal complex component of Toxoplasma gondii identified by proximity-based proteomics as part of one of four architecturally distinct basal complex subcomplexes involved in cell division, but its specific molecular function within the complex has not yet been experimentally defined."},"narrative":{"mechanistic_narrative":"BCC11 is a component of the Toxoplasma gondii basal complex, identified through reciprocal proximity-based biotinylation (BioID) proteomics that mapped the basal complex into four architecturally distinct subcomplexes whose composition changes dynamically in correlation with basal complex function during cell division [PMID:35941170]. Beyond its assignment to one of these subcomplexes [PMID:35941170], no further mechanistic detail about BCC11's specific molecular function has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"To resolve the composition and architecture of the Toxoplasma basal complex, proximity proteomics placed BCC11 within one of four distinct subcomplexes, establishing it as a bona fide basal complex constituent linked to cell division.","evidence":"Reciprocal proximity-based biotinylation (BioID) proteomics with spatiotemporal disruption of component expression in Toxoplasma gondii","pmids":["35941170"],"confidence":"Medium","gaps":["BCC11's specific molecular function within the basal complex is not defined","No direct binding partners or substrate of BCC11 identified","The consequence of BCC11 loss on basal complex function or division is not individually reported"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii basal complex (one of four architecturally distinct subcomplexes)"],"partners":[],"other_free_text":[]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"35941170","id":"PMC_35941170","title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.","date":"2022","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/35941170","citation_count":46,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2793,"output_tokens":327,"usd":0.006642,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4907,"output_tokens":1112,"usd":0.026167,"stage2_stop_reason":"end_turn"},"total_usd":0.033596,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"BCC11 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach, placing it within one of four architecturally distinct BC subcomplexes whose composition changes dynamically in correlation with changes in BC function during cell division.\",\n      \"method\": \"Reciprocal proximity-based biotinylation (BioID) proteomics; spatiotemporal disruption of component expression\",\n      \"journal\": \"Nature Communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — reciprocal BioID proteomics with functional follow-up, but BCC11-specific mechanistic details are not individually reported beyond its inclusion in the BC proteome map\",\n      \"pmids\": [\"35941170\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"BCC11 is a basal complex component of Toxoplasma gondii identified by proximity-based proteomics as part of one of four architecturally distinct basal complex subcomplexes involved in cell division, but its specific molecular function within the complex has not yet been experimentally defined.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"BCC11 is a component of the Toxoplasma gondii basal complex, identified through reciprocal proximity-based biotinylation (BioID) proteomics that mapped the basal complex into four architecturally distinct subcomplexes whose composition changes dynamically in correlation with basal complex function during cell division [#0]. Beyond its assignment to one of these subcomplexes [#0], no further mechanistic detail about BCC11's specific molecular function has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"To resolve the composition and architecture of the Toxoplasma basal complex, proximity proteomics placed BCC11 within one of four distinct subcomplexes, establishing it as a bona fide basal complex constituent linked to cell division.\",\n      \"evidence\": \"Reciprocal proximity-based biotinylation (BioID) proteomics with spatiotemporal disruption of component expression in Toxoplasma gondii\",\n      \"pmids\": [\n        \"35941170\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"BCC11's specific molecular function within the basal complex is not defined\",\n        \"No direct binding partners or substrate of BCC11 identified\",\n        \"The consequence of BCC11 loss on basal complex function or division is not individually reported\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular role of BCC11 within its basal complex subcomplex, and its individual contribution to basal complex function during cell division, remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No mechanistic function assigned to BCC11 individually\",\n        \"No structural or interaction data specific to BCC11\",\n        \"No loss-of-function phenotype reported for BCC11 alone\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"basal complex\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"cell division\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"Toxoplasma gondii basal complex (one of four architecturally distinct subcomplexes)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": []\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_278130","name":"BCC11","product":"basal complex component BCC11","uniprot_acc":"S8GAK9","chromosome":"TGME49_chrXII","start_pos":6269916},{"strain":"GT1","gene_id":"TGGT1_278130","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrXII","start_pos":6183644},{"strain":"RH88","gene_id":"TGRH88_065660","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023093","start_pos":6347045},{"strain":"VEG","gene_id":"TGVEG_278130","name":null,"product":"hypothetical protein","uniprot_acc":"V5BH97","chromosome":"TGVEG_chrXII","start_pos":6277938}]}