{"gene":"TG_274160","gene_symbol":"PCR1","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"Preconoidal rings (PCRs), which include PCR1, serve as a hub for glideosome components including Formin1, as established by ultrastructure expansion microscopy.","method":"Ultrastructure expansion microscopy (UExM)","journal":"Nature microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization experiment with functional consequence, single lab but orthogonal imaging approach","pmids":["36109645"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"PCR1 is a component of the preconoidal rings (PCRs) in Toxoplasma gondii, which serve as a hub for glideosome components including Formin1, thereby linking the apical cytoskeletal structure to the actomyosin machinery that controls parasite motility, invasion, and egress."},"narrative":{"mechanistic_narrative":"PCR1 is a component of the preconoidal rings (PCRs) at the apical tip of Toxoplasma gondii, cytoskeletal structures that serve as a hub concentrating glideosome components including Formin1 [PMID:36109645]. Through this localization the PCRs, and PCR1 as one of their constituents, link the apical cytoskeleton to the actomyosin machinery underlying parasite motility [PMID:36109645]. Beyond this ultrastructural localization within the PCR, no further mechanistic detail about PCR1 itself has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Whether the preconoidal rings have a defined molecular composition and functional role was unresolved; identifying PCR1 as a PCR component that co-organizes glideosome factors established the PCR as an apical hub coupling the cytoskeleton to the motility machinery.","evidence":"Ultrastructure expansion microscopy (UExM) localizing PCR1 and glideosome components including Formin1 in Toxoplasma gondii","pmids":["36109645"],"confidence":"Medium","gaps":["The specific molecular function of PCR1 within the PCR is not defined","Direct binding partners and the mechanism by which PCR1 recruits or organizes glideosome components are not established","No loss-of-function phenotype for PCR1 itself is characterized"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["preconoidal rings (PCRs)"],"partners":["FORMIN1 (CO-LOCALIZED GLIDEOSOME COMPONENT AT THE PCR)"],"other_free_text":[]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"36109645","id":"PMC_36109645","title":"Conoid extrusion regulates glideosome assembly to control motility and invasion in Apicomplexa.","date":"2022","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/36109645","citation_count":37,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2848,"output_tokens":929,"usd":0.011239,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5787,"output_tokens":2056,"usd":0.040168,"stage2_stop_reason":"end_turn"},"total_usd":0.085293,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"Preconoidal rings (PCRs), which include PCR1, serve as a hub for glideosome components including Formin1, as established by ultrastructure expansion microscopy.\",\n      \"method\": \"Ultrastructure expansion microscopy (UExM)\",\n      \"journal\": \"Nature microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization experiment with functional consequence, single lab but orthogonal imaging approach\",\n      \"pmids\": [\"36109645\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PCR1 is a component of the preconoidal rings (PCRs) in Toxoplasma gondii, which serve as a hub for glideosome components including Formin1, thereby linking the apical cytoskeletal structure to the actomyosin machinery that controls parasite motility, invasion, and egress.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PCR1 is a component of the preconoidal rings (PCRs) at the apical tip of Toxoplasma gondii, cytoskeletal structures that serve as a hub concentrating glideosome components including Formin1 [#0]. Through this localization the PCRs, and PCR1 as one of their constituents, link the apical cytoskeleton to the actomyosin machinery underlying parasite motility [#0]. Beyond this ultrastructural localization within the PCR, no further mechanistic detail about PCR1 itself has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Whether the preconoidal rings have a defined molecular composition and functional role was unresolved; identifying PCR1 as a PCR component that co-organizes glideosome factors established the PCR as an apical hub coupling the cytoskeleton to the motility machinery.\",\n      \"evidence\": \"Ultrastructure expansion microscopy (UExM) localizing PCR1 and glideosome components including Formin1 in Toxoplasma gondii\",\n      \"pmids\": [\n        \"36109645\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The specific molecular function of PCR1 within the PCR is not defined\",\n        \"Direct binding partners and the mechanism by which PCR1 recruits or organizes glideosome components are not established\",\n        \"No loss-of-function phenotype for PCR1 itself is characterized\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The intrinsic biochemical activity of PCR1, its direct interaction partners, and its individual contribution to PCR assembly or parasite motility remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct binding partner of PCR1 identified\",\n        \"No structural or biochemical characterization of PCR1\",\n        \"No PCR1-specific functional or genetic perturbation reported\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"preconoidal ring\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"gliding motility / glideosome\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"preconoidal rings (PCRs)\"\n    ],\n    \"partners\": [\n      \"Formin1 (co-localized glideosome component at the PCR)\"\n    ],\n    \"other_free_text\": []\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_274160","name":"PCR1","product":"preconoidal ring protein PCR1","uniprot_acc":"S8F5F5","chromosome":"TGME49_chrVIII","start_pos":2960267},{"strain":"GT1","gene_id":"TGGT1_274160","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":2959042},{"strain":"RH88","gene_id":"TGRH88_077870","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":8068986},{"strain":"VEG","gene_id":"TGVEG_274160","name":null,"product":"hypothetical protein","uniprot_acc":"A0A0F7V4I7","chromosome":"TGVEG_chrVIII","start_pos":2971366}]}