{"gene":"TG_273050","gene_symbol":"BCC8","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"BCC8 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach; the BC is organized into four architecturally distinct subcomplexes whose compositions change dynamically in correlation with changes in BC function during cell division.","method":"Reciprocal proximity-based biotinylation (BioID) proteomics; disruption of component expression with spatiotemporal and functional readouts","journal":"Nature Communications","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal BioID proteomics with functional disruption experiments in a single study; BCC8 is listed as a BC component but no specific functional experiment is described for BCC8 alone","pmids":["35941170"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"BCC8 is a basal complex component in Toxoplasma gondii, identified by proximity-based proteomics as part of one of four architecturally distinct subcomplexes whose composition changes dynamically during cell division, but its specific molecular function within the basal complex has not yet been defined."},"narrative":{"mechanistic_narrative":"BCC8 is a component of the Toxoplasma gondii basal complex, a cytoskeletal structure identified through reciprocal proximity-based biotinylation (BioID) proteomics as being organized into four architecturally distinct subcomplexes whose compositions change dynamically in correlation with changes in basal complex function during cell division [PMID:35941170]. Beyond its assignment as a basal complex component in this proteomic survey, no specific molecular function has been characterized for BCC8 in the available corpus.","teleology":[{"year":2022,"claim":"Before this work the full protein composition of the Toxoplasma basal complex was unresolved; mapping it established BCC8 as a bona fide basal complex component within one of four dynamically changing subcomplexes.","evidence":"Reciprocal proximity-based biotinylation (BioID) proteomics with component disruption and spatiotemporal/functional readouts in Toxoplasma gondii","pmids":["35941170"],"confidence":"Medium","gaps":["No functional experiment specific to BCC8 alone was performed","The subcomplex assignment and precise substructure localization of BCC8 within the basal complex is not defined","The molecular activity and interaction partners of BCC8 are unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii basal complex"],"partners":[],"other_free_text":["Identified by reciprocal BioID proteomics as part of one of four architecturally distinct basal complex subcomplexes whose composition changes dynamically during cell division [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"35941170","id":"PMC_35941170","title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.","date":"2022","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/35941170","citation_count":46,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2793,"output_tokens":364,"usd":0.00692,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4953,"output_tokens":1027,"usd":0.02522,"stage2_stop_reason":"end_turn"},"total_usd":0.034084,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"BCC8 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach; the BC is organized into four architecturally distinct subcomplexes whose compositions change dynamically in correlation with changes in BC function during cell division.\",\n      \"method\": \"Reciprocal proximity-based biotinylation (BioID) proteomics; disruption of component expression with spatiotemporal and functional readouts\",\n      \"journal\": \"Nature Communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal BioID proteomics with functional disruption experiments in a single study; BCC8 is listed as a BC component but no specific functional experiment is described for BCC8 alone\",\n      \"pmids\": [\"35941170\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"BCC8 is a basal complex component in Toxoplasma gondii, identified by proximity-based proteomics as part of one of four architecturally distinct subcomplexes whose composition changes dynamically during cell division, but its specific molecular function within the basal complex has not yet been defined.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"BCC8 is a component of the Toxoplasma gondii basal complex, a cytoskeletal structure identified through reciprocal proximity-based biotinylation (BioID) proteomics as being organized into four architecturally distinct subcomplexes whose compositions change dynamically in correlation with changes in basal complex function during cell division [#0]. Beyond its assignment as a basal complex component in this proteomic survey, no specific molecular function has been characterized for BCC8 in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Before this work the full protein composition of the Toxoplasma basal complex was unresolved; mapping it established BCC8 as a bona fide basal complex component within one of four dynamically changing subcomplexes.\",\n      \"evidence\": \"Reciprocal proximity-based biotinylation (BioID) proteomics with component disruption and spatiotemporal/functional readouts in Toxoplasma gondii\",\n      \"pmids\": [\n        \"35941170\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional experiment specific to BCC8 alone was performed\",\n        \"The subcomplex assignment and precise substructure localization of BCC8 within the basal complex is not defined\",\n        \"The molecular activity and interaction partners of BCC8 are unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of BCC8 within the basal complex and its contribution to cell division remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No dedicated loss-of-function or biochemical characterization of BCC8\",\n        \"No defined direct binding partners or enzymatic/structural role\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"basal complex\",\n        \"supporting_discovery_ids\": [\n          0\n        ],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"cell division\",\n        \"supporting_discovery_ids\": [\n          0\n        ],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"Toxoplasma gondii basal complex\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified by reciprocal BioID proteomics as part of one of four architecturally distinct basal complex subcomplexes whose composition changes dynamically during cell division [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_273050","name":"BCC8","product":"basal complex component BCC8","uniprot_acc":"S8GA19","chromosome":"TGME49_chrVIII","start_pos":3692313},{"strain":"GT1","gene_id":"TGGT1_273050","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":3689301},{"strain":"RH88","gene_id":"TGRH88_078890","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":8800771},{"strain":"VEG","gene_id":"TGVEG_273050","name":null,"product":"hypothetical protein","uniprot_acc":"V4ZLM6","chromosome":"TGVEG_chrVIII","start_pos":3701569}]}