{"gene":"TG_269960","gene_symbol":"IMC40","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2023,"finding":"IMC40 was identified as one of eight novel IMC proteins enriched in daughter buds, expanding the daughter IMC proteome, via proximity labeling centered on IMC29.","method":"Proximity labeling (BioID) using IMC29 as bait, followed by mass spectrometry identification","journal":"mBio","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single proximity-labeling experiment identifying IMC40 as a candidate daughter-enriched IMC protein; no functional characterization of IMC40 itself reported","pmids":["36622147"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC40 is a novel inner membrane complex protein enriched in daughter buds during Toxoplasma endodyogeny, identified by proximity labeling, but its specific molecular function remains uncharacterized."},"narrative":{"mechanistic_narrative":"IMC40 is a novel inner membrane complex (IMC) protein of Toxoplasma gondii identified as one of eight daughter-bud-enriched proteins that expand the daughter IMC proteome, detected by proximity labeling centered on IMC29 [PMID:36622147]. Beyond this proximity-based identification and its daughter-bud enrichment, no molecular function for IMC40 has been characterized in the available corpus.","teleology":[{"year":2023,"claim":"To expand the catalog of proteins operating at the daughter inner membrane complex during endodyogeny, IMC40 was placed within the daughter IMC proteome as a proximity partner of IMC29.","evidence":"BioID proximity labeling using IMC29 as bait followed by mass spectrometry in Toxoplasma gondii","pmids":["36622147"],"confidence":"Low","gaps":["Single proximity-labeling experiment with no functional characterization of IMC40 itself","Direct interaction versus mere proximity to IMC29 not resolved","No knockout, localization detail, or role in bud formation established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["IMC29 (PROXIMITY PARTNER IN BIOID)"],"other_free_text":["Enriched in daughter buds during endodyogeny [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"36622147","id":"PMC_36622147","title":"IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome.","date":"2023","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/36622147","citation_count":30,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3105,"output_tokens":300,"usd":0.006907,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4880,"output_tokens":941,"usd":0.023963,"stage2_stop_reason":"end_turn"},"total_usd":0.032117,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC40 was identified as one of eight novel IMC proteins enriched in daughter buds, expanding the daughter IMC proteome, via proximity labeling centered on IMC29.\",\n      \"method\": \"Proximity labeling (BioID) using IMC29 as bait, followed by mass spectrometry identification\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single proximity-labeling experiment identifying IMC40 as a candidate daughter-enriched IMC protein; no functional characterization of IMC40 itself reported\",\n      \"pmids\": [\"36622147\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC40 is a novel inner membrane complex protein enriched in daughter buds during Toxoplasma endodyogeny, identified by proximity labeling, but its specific molecular function remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC40 is a novel inner membrane complex (IMC) protein of Toxoplasma gondii identified as one of eight daughter-bud-enriched proteins that expand the daughter IMC proteome, detected by proximity labeling centered on IMC29 [#0]. Beyond this proximity-based identification and its daughter-bud enrichment, no molecular function for IMC40 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2023,\n      \"claim\": \"To expand the catalog of proteins operating at the daughter inner membrane complex during endodyogeny, IMC40 was placed within the daughter IMC proteome as a proximity partner of IMC29.\",\n      \"evidence\": \"BioID proximity labeling using IMC29 as bait followed by mass spectrometry in Toxoplasma gondii\",\n      \"pmids\": [\n        \"36622147\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Single proximity-labeling experiment with no functional characterization of IMC40 itself\",\n        \"Direct interaction versus mere proximity to IMC29 not resolved\",\n        \"No knockout, localization detail, or role in bud formation established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of IMC40, its precise localization within the IMC, and its contribution to daughter bud assembly remain unknown.\",\n      \"evidence\": \"No further discoveries in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No functional assay linking IMC40 to a defined process\",\n        \"No binding partners beyond proximity to IMC29 defined\",\n        \"No essentiality or phenotype data\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"inner membrane complex\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\n      \"IMC29 (proximity partner in BioID)\"\n    ],\n    \"other_free_text\": [\n      \"Enriched in daughter buds during endodyogeny [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_269960","name":"IMC40","product":"inner membrane complex protein IMC40","uniprot_acc":"S8F1B8","chromosome":"TGME49_chrVIII","start_pos":5493679},{"strain":"GT1","gene_id":"TGGT1_269960","name":null,"product":"14-3-3 superfamily protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":5492015},{"strain":"RH88","gene_id":"TGRH88_081390","name":null,"product":"14-3-3 superfamily protein","uniprot_acc":null,"chromosome":"CM023089","start_pos":10612691},{"strain":"VEG","gene_id":"TGVEG_269960","name":null,"product":"14-3-3 superfamily protein","uniprot_acc":"V4Z7W0","chromosome":"TGVEG_chrVIII","start_pos":5507860}]}