{"gene":"TG_269460","gene_symbol":"BCC5","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"BCC5 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach that mapped the BC and its four architecturally distinct subcomplexes with spatiotemporal dynamics.","method":"Reciprocal proximity-based biotinylation (BioID) proteomics of the basal complex","journal":"Nature communications","confidence":"Low","confidence_rationale":"Tier 3 / Weak — BCC5 is identified as a BC component by proteomics mapping but no specific functional experiment is described for BCC5 itself; single method, single lab","pmids":["35941170"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"BCC5 (TG_269460) has been identified as a component of the Toxoplasma gondii basal complex proteome via proximity biotinylation, placing it within the basal complex machinery involved in cell division, but no specific molecular mechanism, binding partner, or functional role has been experimentally defined for BCC5 itself."},"narrative":{"mechanistic_narrative":"BCC5 is a component of the Toxoplasma gondii basal complex, identified within the basal complex proteome through reciprocal proximity-based biotinylation (BioID) mapping of the basal complex and its architecturally distinct subcomplexes [PMID:35941170]. Beyond this proteomic assignment, no specific molecular activity, binding partner, or functional role has been experimentally defined for BCC5 in the available corpus.","teleology":[{"year":2022,"claim":"Establishing which proteins constitute the Toxoplasma basal complex was needed to define the machinery of parasite cell division; BioID mapping placed BCC5 among the basal complex components.","evidence":"Reciprocal proximity-based biotinylation (BioID) proteomics of the basal complex in T. gondii","pmids":["35941170"],"confidence":"Low","gaps":["No functional experiment on BCC5 itself; assignment rests on proximity proteomics from a single method and lab","No binding partner, substrate, or molecular activity defined for BCC5","Precise subcomplex localization and role in division not established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii basal complex"],"partners":[],"other_free_text":[]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"35941170","id":"PMC_35941170","title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.","date":"2022","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/35941170","citation_count":46,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2793,"output_tokens":340,"usd":0.006739,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4924,"output_tokens":974,"usd":0.024485,"stage2_stop_reason":"end_turn"},"total_usd":0.03104,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"BCC5 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach that mapped the BC and its four architecturally distinct subcomplexes with spatiotemporal dynamics.\",\n      \"method\": \"Reciprocal proximity-based biotinylation (BioID) proteomics of the basal complex\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — BCC5 is identified as a BC component by proteomics mapping but no specific functional experiment is described for BCC5 itself; single method, single lab\",\n      \"pmids\": [\"35941170\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"BCC5 (TG_269460) has been identified as a component of the Toxoplasma gondii basal complex proteome via proximity biotinylation, placing it within the basal complex machinery involved in cell division, but no specific molecular mechanism, binding partner, or functional role has been experimentally defined for BCC5 itself.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"BCC5 is a component of the Toxoplasma gondii basal complex, identified within the basal complex proteome through reciprocal proximity-based biotinylation (BioID) mapping of the basal complex and its architecturally distinct subcomplexes [#0]. Beyond this proteomic assignment, no specific molecular activity, binding partner, or functional role has been experimentally defined for BCC5 in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Establishing which proteins constitute the Toxoplasma basal complex was needed to define the machinery of parasite cell division; BioID mapping placed BCC5 among the basal complex components.\",\n      \"evidence\": \"Reciprocal proximity-based biotinylation (BioID) proteomics of the basal complex in T. gondii\",\n      \"pmids\": [\"35941170\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No functional experiment on BCC5 itself; assignment rests on proximity proteomics from a single method and lab\",\n        \"No binding partner, substrate, or molecular activity defined for BCC5\",\n        \"Precise subcomplex localization and role in division not established\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function, direct interaction partners, and role of BCC5 within the basal complex remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No enzymatic activity has been demonstrated for BCC5\",\n        \"No direct partner or subcomplex assignment confirmed\",\n        \"No knockout/knockdown phenotype reported\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"basal complex\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"Toxoplasma gondii basal complex\"],\n    \"partners\": [],\n    \"other_free_text\": []\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_269460","name":"BCC5","product":"basal complex component BCC5","uniprot_acc":"S8G9D7","chromosome":"TGME49_chrVIII","start_pos":5777195},{"strain":"GT1","gene_id":"TGGT1_269460","name":null,"product":"Ser/Thr phosphatase family protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":5776112},{"strain":"RH88","gene_id":"TGRH88_081750","name":null,"product":"Ser/Thr phosphatase family protein","uniprot_acc":null,"chromosome":"CM023089","start_pos":10896107},{"strain":"VEG","gene_id":"TGVEG_269460","name":null,"product":"Ser/Thr phosphatase family protein","uniprot_acc":"V5BMW2","chromosome":"TGVEG_chrVIII","start_pos":5792880}]}