{"gene":"TG_268840","gene_symbol":"METTL14","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2021,"finding":"METTL14 was identified as a novel component of the m6A methyltransferase complex in Toxoplasma gondii; the complex deposits N6-methyladenosine (m6A) marks preferentially near the 3'-boundary of mRNA transcripts.","method":"Identification of m6A methyltransferase complex components combined with transcriptome-wide m6A mapping","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — single lab, complex-level identification with m6A mapping; METTL14 named as component but functional knockdown experiments reported only for METTL3 and WTAP, not METTL14 specifically","pmids":["34324585"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"METTL14 is a component of the m6A mRNA methyltransferase complex in Toxoplasma gondii, which deposits N6-methyladenosine marks preferentially near the 3'-ends of mRNA transcripts; this complex activity is linked to pre-mRNA 3'-end formation and parasite viability, though direct functional experiments on METTL14 itself (as distinct from other complex members METTL3 and WTAP) have not yet been reported."},"narrative":{"mechanistic_narrative":"METTL14 is a component of the N6-methyladenosine (m6A) mRNA methyltransferase complex in Toxoplasma gondii, a complex that deposits m6A marks preferentially near the 3'-boundary of mRNA transcripts [PMID:34324585]. Beyond its identification as a complex component [PMID:34324585], no METTL14-specific mechanistic detail has been characterized in the available corpus.","teleology":[{"year":2021,"claim":"Whether an m6A methyltransferase machinery exists in Toxoplasma gondii and what its members are was unknown; identifying METTL14 as a component and mapping m6A transcriptome-wide established a parasite m6A writer complex that marks transcripts preferentially near their 3'-ends.","evidence":"Identification of m6A methyltransferase complex components combined with transcriptome-wide m6A mapping in Toxoplasma gondii","pmids":["34324585"],"confidence":"Medium","gaps":["No functional knockdown or knockout of METTL14 itself was reported; loss-of-function was shown only for other complex members (METTL3, WTAP)","The specific molecular contribution of METTL14 (catalytic versus scaffolding/non-catalytic) within the complex was not defined","No structural or interaction-mapping data localizing METTL14 within the complex were provided"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["m6A mRNA methyltransferase complex (Toxoplasma gondii)"],"partners":["METTL3","WTAP"],"other_free_text":["m6A marks deposited preferentially near the 3'-boundary of mRNA transcripts [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"34324585","id":"PMC_34324585","title":"m6A RNA methylation facilitates pre-mRNA 3'-end formation and is essential for viability of Toxoplasma gondii.","date":"2021","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/34324585","citation_count":27,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2914,"output_tokens":918,"usd":0.011256,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5731,"output_tokens":1758,"usd":0.036303,"stage2_stop_reason":"end_turn"},"total_usd":0.083093,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2021,\n      \"finding\": \"METTL14 was identified as a novel component of the m6A methyltransferase complex in Toxoplasma gondii; the complex deposits N6-methyladenosine (m6A) marks preferentially near the 3'-boundary of mRNA transcripts.\",\n      \"method\": \"Identification of m6A methyltransferase complex components combined with transcriptome-wide m6A mapping\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — single lab, complex-level identification with m6A mapping; METTL14 named as component but functional knockdown experiments reported only for METTL3 and WTAP, not METTL14 specifically\",\n      \"pmids\": [\"34324585\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"METTL14 is a component of the m6A mRNA methyltransferase complex in Toxoplasma gondii, which deposits N6-methyladenosine marks preferentially near the 3'-ends of mRNA transcripts; this complex activity is linked to pre-mRNA 3'-end formation and parasite viability, though direct functional experiments on METTL14 itself (as distinct from other complex members METTL3 and WTAP) have not yet been reported.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"METTL14 is a component of the N6-methyladenosine (m6A) mRNA methyltransferase complex in Toxoplasma gondii, a complex that deposits m6A marks preferentially near the 3'-boundary of mRNA transcripts [#0]. Beyond its identification as a complex component [#0], no METTL14-specific mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2021,\n      \"claim\": \"Whether an m6A methyltransferase machinery exists in Toxoplasma gondii and what its members are was unknown; identifying METTL14 as a component and mapping m6A transcriptome-wide established a parasite m6A writer complex that marks transcripts preferentially near their 3'-ends.\",\n      \"evidence\": \"Identification of m6A methyltransferase complex components combined with transcriptome-wide m6A mapping in Toxoplasma gondii\",\n      \"pmids\": [\n        \"34324585\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional knockdown or knockout of METTL14 itself was reported; loss-of-function was shown only for other complex members (METTL3, WTAP)\",\n        \"The specific molecular contribution of METTL14 (catalytic versus scaffolding/non-catalytic) within the complex was not defined\",\n        \"No structural or interaction-mapping data localizing METTL14 within the complex were provided\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct, METTL14-specific functional role in m6A deposition, target selection, and parasite viability remains uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No METTL14-specific loss-of-function phenotype established\",\n        \"No defined substrate specificity or mechanism of recruitment for METTL14\",\n        \"No structural model of METTL14 within the T. gondii m6A complex\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\n        \"term_id\": \"GO:0016422\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"localization\": [],\n    \"pathway\": [\n      {\n        \"term_id\": \"GO:0080009\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"m6A mRNA methyltransferase complex (Toxoplasma gondii)\"\n    ],\n    \"partners\": [\n      \"METTL3\",\n      \"WTAP\"\n    ],\n    \"other_free_text\": [\n      \"m6A marks deposited preferentially near the 3'-boundary of mRNA transcripts [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_268840","name":"METTL14","product":"N6-adenosine-methyltransferase non-catalytic subunit METTL14","uniprot_acc":"S8GII7","chromosome":"TGME49_chrVIII","start_pos":6254339},{"strain":"GT1","gene_id":"TGGT1_268840","name":null,"product":"putative N6-adenosine-methyltransferase","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":6253001},{"strain":"RH88","gene_id":"TGRH88_082410","name":null,"product":"putative N6-adenosine-methyltransferase","uniprot_acc":null,"chromosome":"CM023089","start_pos":11373083},{"strain":"VEG","gene_id":"TGVEG_268840","name":null,"product":"putative N6-adenosine-methyltransferase","uniprot_acc":"B9Q598","chromosome":"TGVEG_chrVIII","start_pos":6270083}]}