{"gene":"TG_267810","gene_symbol":"RAB5A","run_date":"2026-09-08T14:08:29","timeline":{"discoveries":[{"year":2013,"finding":"Overexpression screen identified TgRab5A as an important regulator of trafficking to micronemes and rhoptries in T. gondii; not all microneme protein traffic depends on functional Rab5A, indicating redundant microneme targeting pathways. Two-colour STED super-resolution microscopy verified distinct localisations of independent microneme proteins, demonstrating that micronemal organelles are organised in distinct subsets.","method":"Rab-GTPase overexpression screen; two-colour super-resolution STED microscopy","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — overexpression screen with super-resolution imaging in a single lab, two orthogonal methods, but no direct loss-of-function or in vitro reconstitution","pmids":["23505371"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2016,"finding":"TgVps9 acts as a guanine nucleotide exchange factor (GEF) for Rab5A in T. gondii and is crucial for sorting of proteins destined to secretory organelles (rhoptries, micronemes, dense granules), thereby regulating protein maturation, secretory organelle formation, and secretion.","method":"GEF activity assay (in vitro biochemical assay demonstrating nucleotide exchange activity toward Rab5A); conditional knockdown/loss-of-function with secretory organelle phenotype readout","journal":"Scientific reports","confidence":"High","confidence_rationale":"Tier 1 / Moderate — direct in vitro GEF activity assay plus loss-of-function phenotypic analysis in a single lab, two orthogonal methods establishing Vps9 as the Rab5A GEF","pmids":["27966671"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2015,"finding":"TgVps11, a subunit of HOPS and CORVET tethering complexes that acts downstream of Rab-GTPases including Rab5A, is essential for biogenesis of secretory organelles (dense granules, rhoptries, micronemes); conditional knockdown of TgVps11 disrupts organelle biogenesis and impairs motility, invasion, egress and intracellular growth.","method":"Conditional knockdown (tetracycline-regulated); immunofluorescence and subcellular fractionation to assess organelle biogenesis; phenotypic assays (invasion, egress, growth)","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — conditional knockdown with multiple phenotypic readouts in a single lab; Rab5A involvement inferred from pathway context rather than direct interaction assay in this paper","pmids":["25640905"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2017,"finding":"TgAP1 has a specific functional relationship with the Rab5A-positive endosome-like compartment during post-Golgi maturation of preROP compartments into apically anchored mature rhoptries; AP1 disruption misroutes microneme proteins at the TGN and impairs rhoptry biogenesis.","method":"CreLox-based inducible knockout; ddFKBP overexpression; immunofluorescence co-localisation with Rab5A marker; secretory organelle biogenesis phenotype readout","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — inducible knockout with co-localisation data placing TgAP1 at Rab5A-positive compartment; single lab, functional link supported by two orthogonal approaches","pmids":["28430827"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"finding":"Microneme organelles in T. gondii comprise two sub-populations distinguished by dependence on Rab5A/C in their biogenesis; TgFER1 acts differentially on these two microneme sub-populations and mediates trafficking from the trans-Golgi-endosomal network to the subpellicular cortex, along the cortex to the apical tip, and fusion with the plasma membrane during exocytosis.","method":"Dominant-negative overexpression of FER1; live imaging; fluorescence microscopy to distinguish Rab5A/C-dependent vs. independent microneme sub-populations; microneme exocytosis assays","journal":"Scientific reports","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — dominant-negative strategy with live imaging and exocytosis assays in a single lab; Rab5A sub-population distinction supported by prior work and confirmed here","pmids":["39294204"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TgRab5A is a Rab-GTPase activated by the GEF TgVps9 that localizes to an endosome-like compartment and regulates trafficking of a subset of microneme and rhoptry proteins from the trans-Golgi network to their respective secretory organelles, acting in concert with downstream tethering factors (HOPS/CORVET via Vps11), the clathrin adaptor TgAP1, and the ferlin FER1, which together coordinate post-Golgi vesicle sorting and organelle biogenesis essential for host cell invasion."},"narrative":{"mechanistic_narrative":"TgRab5A is a Rab-GTPase that governs post-Golgi trafficking of a subset of secretory organelle proteins to micronemes and rhoptries in Toxoplasma gondii, thereby regulating biogenesis of the invasion organelles [PMID:23505371]. It is activated by the guanine nucleotide exchange factor TgVps9, whose loss disrupts sorting of proteins destined for rhoptries, micronemes, and dense granules and impairs organelle maturation and secretion [PMID:27966671]. TgRab5A defines an endosome-like compartment through which post-Golgi maturation proceeds: the clathrin adaptor TgAP1 acts at this Rab5A-positive compartment during conversion of preROP compartments into mature apically anchored rhoptries, and AP1 disruption misroutes microneme proteins at the trans-Golgi network [PMID:28430827]. Rab5A dependence subdivides the micronemes into two biogenetically distinct sub-populations, on which the ferlin TgFER1 acts differentially to mediate trafficking from the trans-Golgi-endosomal network to the cortex and subsequent exocytic fusion [PMID:39294204]. Downstream tethering is inferred to involve the HOPS/CORVET subunit TgVps11, which is required for secretory organelle biogenesis and for motility, invasion, egress, and growth [PMID:25640905]. No structural model of TgRab5A or direct effector-binding data has been characterized in the available corpus.","teleology":[{"year":2013,"claim":"Established that a Rab-GTPase controls secretory organelle trafficking in T. gondii, answering which regulators route proteins to micronemes and rhoptries and revealing that this pathway serves only a subset of cargo.","evidence":"Rab-GTPase overexpression screen with two-colour STED super-resolution microscopy in T. gondii","pmids":["23505371"],"confidence":"Medium","gaps":["No direct loss-of-function of Rab5A itself","Identity of the redundant, Rab5A-independent microneme targeting pathway unknown","No in vitro reconstitution or direct effector identification"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2015,"claim":"Placed a HOPS/CORVET tethering subunit downstream of Rab-GTPases in the pathway, addressing how tethering machinery contributes to organelle biogenesis and invasion phenotypes.","evidence":"Tetracycline-regulated conditional knockdown of TgVps11 with immunofluorescence, subcellular fractionation, and invasion/egress/growth assays in T. gondii","pmids":["25640905"],"confidence":"Medium","gaps":["Rab5A involvement inferred from pathway context, not a direct interaction assay","No demonstration of physical Rab5A-Vps11 coupling"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2016,"claim":"Identified the upstream activator of Rab5A, answering how the GTPase is switched on and linking that activation to sorting, maturation, and secretion of secretory organelle proteins.","evidence":"In vitro GEF nucleotide-exchange assay toward Rab5A plus conditional loss-of-function of TgVps9 with secretory organelle phenotype readouts in T. gondii","pmids":["27966671"],"confidence":"High","gaps":["Downstream Rab5A effectors not identified","Whether Vps9 acts on Rab5A exclusively or additional Rabs is unresolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2017,"claim":"Defined the functional relationship between the Rab5A-positive endosome-like compartment and clathrin adaptor sorting, showing how post-Golgi maturation of rhoptries and correct microneme routing depend on AP1 at this compartment.","evidence":"CreLox inducible knockout and ddFKBP overexpression of TgAP1 with immunofluorescence co-localisation to the Rab5A marker in T. gondii","pmids":["28430827"],"confidence":"Medium","gaps":["Direct physical interaction between AP1 and Rab5A not shown","Molecular mechanism of preROP-to-rhoptry maturation unresolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"claim":"Resolved that micronemes comprise two Rab5A/C-dependence-defined sub-populations and identified a ferlin acting differentially on them, answering how distinct microneme cargo is trafficked and exocytosed.","evidence":"Dominant-negative overexpression of TgFER1 with live imaging and microneme exocytosis assays in T. gondii","pmids":["39294204"],"confidence":"Medium","gaps":["Dominant-negative rather than clean loss-of-function","Mechanism distinguishing Rab5A-dependent vs -independent microneme sub-populations unknown","Direct Rab5A-FER1 coupling not established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The direct effectors of activated TgRab5A and the structural basis of its nucleotide switching remain unidentified.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No direct Rab5A effector or interactor mapped biochemically","No structural model of TgRab5A","GTP/GDP cycling of Rab5A in vivo not directly tracked"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["TGVPS9 (GEF ACTIVATING RAB5A)","TGVPS11 (HOPS/CORVET TETHERING SUBUNIT, DOWNSTREAM)","TGAP1 (CLATHRIN ADAPTOR AT RAB5A-POSITIVE COMPARTMENT)","TGFER1 (FERLIN ACTING ON RAB5A/C-DEPENDENT MICRONEMES)"],"other_free_text":["Regulates trafficking of a subset of microneme and rhoptry proteins from the TGN to secretory organelles; redundant Rab5A-independent microneme targeting pathways exist.","Rab5A/C dependence distinguishes two biogenetically distinct microneme sub-populations."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"23505371","id":"PMC_23505371","title":"An overexpression screen of Toxoplasma gondii Rab-GTPases reveals distinct transport routes to the micronemes.","date":"2013","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/23505371","citation_count":122,"is_preprint":false},{"pmid":"25640905","id":"PMC_25640905","title":"Toxoplasma gondii Vps11, a subunit of HOPS and CORVET tethering complexes, is essential for the biogenesis of secretory organelles.","date":"2015","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/25640905","citation_count":45,"is_preprint":false},{"pmid":"28430827","id":"PMC_28430827","title":"Dual role of the Toxoplasma gondii clathrin adaptor AP1 in the sorting of rhoptry and microneme proteins and in parasite division.","date":"2017","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/28430827","citation_count":41,"is_preprint":false},{"pmid":"27966671","id":"PMC_27966671","title":"A Critical Role for Toxoplasma gondii Vacuolar Protein Sorting VPS9 in Secretory Organelle Biogenesis and Host Infection.","date":"2016","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/27966671","citation_count":27,"is_preprint":false},{"pmid":"39294204","id":"PMC_39294204","title":"Toxoplasma FER1 is a versatile and dynamic mediator of differential microneme trafficking and microneme exocytosis.","date":"2024","source":"Scientific reports","url":"https://pubmed.ncbi.nlm.nih.gov/39294204","citation_count":7,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":4822,"output_tokens":1572,"usd":0.019023,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6669,"output_tokens":2745,"usd":0.050985,"stage2_stop_reason":"end_turn"},"total_usd":0.070008,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2013,\n      \"finding\": \"Overexpression screen identified TgRab5A as an important regulator of trafficking to micronemes and rhoptries in T. gondii; not all microneme protein traffic depends on functional Rab5A, indicating redundant microneme targeting pathways. Two-colour STED super-resolution microscopy verified distinct localisations of independent microneme proteins, demonstrating that micronemal organelles are organised in distinct subsets.\",\n      \"method\": \"Rab-GTPase overexpression screen; two-colour super-resolution STED microscopy\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — overexpression screen with super-resolution imaging in a single lab, two orthogonal methods, but no direct loss-of-function or in vitro reconstitution\",\n      \"pmids\": [\"23505371\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"TgVps9 acts as a guanine nucleotide exchange factor (GEF) for Rab5A in T. gondii and is crucial for sorting of proteins destined to secretory organelles (rhoptries, micronemes, dense granules), thereby regulating protein maturation, secretory organelle formation, and secretion.\",\n      \"method\": \"GEF activity assay (in vitro biochemical assay demonstrating nucleotide exchange activity toward Rab5A); conditional knockdown/loss-of-function with secretory organelle phenotype readout\",\n      \"journal\": \"Scientific reports\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 1 / Moderate — direct in vitro GEF activity assay plus loss-of-function phenotypic analysis in a single lab, two orthogonal methods establishing Vps9 as the Rab5A GEF\",\n      \"pmids\": [\"27966671\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2015,\n      \"finding\": \"TgVps11, a subunit of HOPS and CORVET tethering complexes that acts downstream of Rab-GTPases including Rab5A, is essential for biogenesis of secretory organelles (dense granules, rhoptries, micronemes); conditional knockdown of TgVps11 disrupts organelle biogenesis and impairs motility, invasion, egress and intracellular growth.\",\n      \"method\": \"Conditional knockdown (tetracycline-regulated); immunofluorescence and subcellular fractionation to assess organelle biogenesis; phenotypic assays (invasion, egress, growth)\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — conditional knockdown with multiple phenotypic readouts in a single lab; Rab5A involvement inferred from pathway context rather than direct interaction assay in this paper\",\n      \"pmids\": [\"25640905\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"finding\": \"TgAP1 has a specific functional relationship with the Rab5A-positive endosome-like compartment during post-Golgi maturation of preROP compartments into apically anchored mature rhoptries; AP1 disruption misroutes microneme proteins at the TGN and impairs rhoptry biogenesis.\",\n      \"method\": \"CreLox-based inducible knockout; ddFKBP overexpression; immunofluorescence co-localisation with Rab5A marker; secretory organelle biogenesis phenotype readout\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — inducible knockout with co-localisation data placing TgAP1 at Rab5A-positive compartment; single lab, functional link supported by two orthogonal approaches\",\n      \"pmids\": [\"28430827\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"Microneme organelles in T. gondii comprise two sub-populations distinguished by dependence on Rab5A/C in their biogenesis; TgFER1 acts differentially on these two microneme sub-populations and mediates trafficking from the trans-Golgi-endosomal network to the subpellicular cortex, along the cortex to the apical tip, and fusion with the plasma membrane during exocytosis.\",\n      \"method\": \"Dominant-negative overexpression of FER1; live imaging; fluorescence microscopy to distinguish Rab5A/C-dependent vs. independent microneme sub-populations; microneme exocytosis assays\",\n      \"journal\": \"Scientific reports\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — dominant-negative strategy with live imaging and exocytosis assays in a single lab; Rab5A sub-population distinction supported by prior work and confirmed here\",\n      \"pmids\": [\"39294204\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"TgRab5A is a Rab-GTPase activated by the GEF TgVps9 that localizes to an endosome-like compartment and regulates trafficking of a subset of microneme and rhoptry proteins from the trans-Golgi network to their respective secretory organelles, acting in concert with downstream tethering factors (HOPS/CORVET via Vps11), the clathrin adaptor TgAP1, and the ferlin FER1, which together coordinate post-Golgi vesicle sorting and organelle biogenesis essential for host cell invasion.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"TgRab5A is a Rab-GTPase that governs post-Golgi trafficking of a subset of secretory organelle proteins to micronemes and rhoptries in Toxoplasma gondii, thereby regulating biogenesis of the invasion organelles [#0]. It is activated by the guanine nucleotide exchange factor TgVps9, whose loss disrupts sorting of proteins destined for rhoptries, micronemes, and dense granules and impairs organelle maturation and secretion [#1]. TgRab5A defines an endosome-like compartment through which post-Golgi maturation proceeds: the clathrin adaptor TgAP1 acts at this Rab5A-positive compartment during conversion of preROP compartments into mature apically anchored rhoptries, and AP1 disruption misroutes microneme proteins at the trans-Golgi network [#3]. Rab5A dependence subdivides the micronemes into two biogenetically distinct sub-populations, on which the ferlin TgFER1 acts differentially to mediate trafficking from the trans-Golgi-endosomal network to the cortex and subsequent exocytic fusion [#4]. Downstream tethering is inferred to involve the HOPS/CORVET subunit TgVps11, which is required for secretory organelle biogenesis and for motility, invasion, egress, and growth [#2]. No structural model of TgRab5A or direct effector-binding data has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2013,\n      \"claim\": \"Established that a Rab-GTPase controls secretory organelle trafficking in T. gondii, answering which regulators route proteins to micronemes and rhoptries and revealing that this pathway serves only a subset of cargo.\",\n      \"evidence\": \"Rab-GTPase overexpression screen with two-colour STED super-resolution microscopy in T. gondii\",\n      \"pmids\": [\"23505371\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct loss-of-function of Rab5A itself\",\n        \"Identity of the redundant, Rab5A-independent microneme targeting pathway unknown\",\n        \"No in vitro reconstitution or direct effector identification\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2015,\n      \"claim\": \"Placed a HOPS/CORVET tethering subunit downstream of Rab-GTPases in the pathway, addressing how tethering machinery contributes to organelle biogenesis and invasion phenotypes.\",\n      \"evidence\": \"Tetracycline-regulated conditional knockdown of TgVps11 with immunofluorescence, subcellular fractionation, and invasion/egress/growth assays in T. gondii\",\n      \"pmids\": [\"25640905\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Rab5A involvement inferred from pathway context, not a direct interaction assay\",\n        \"No demonstration of physical Rab5A-Vps11 coupling\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Identified the upstream activator of Rab5A, answering how the GTPase is switched on and linking that activation to sorting, maturation, and secretion of secretory organelle proteins.\",\n      \"evidence\": \"In vitro GEF nucleotide-exchange assay toward Rab5A plus conditional loss-of-function of TgVps9 with secretory organelle phenotype readouts in T. gondii\",\n      \"pmids\": [\"27966671\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Downstream Rab5A effectors not identified\",\n        \"Whether Vps9 acts on Rab5A exclusively or additional Rabs is unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2017,\n      \"claim\": \"Defined the functional relationship between the Rab5A-positive endosome-like compartment and clathrin adaptor sorting, showing how post-Golgi maturation of rhoptries and correct microneme routing depend on AP1 at this compartment.\",\n      \"evidence\": \"CreLox inducible knockout and ddFKBP overexpression of TgAP1 with immunofluorescence co-localisation to the Rab5A marker in T. gondii\",\n      \"pmids\": [\"28430827\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct physical interaction between AP1 and Rab5A not shown\",\n        \"Molecular mechanism of preROP-to-rhoptry maturation unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Resolved that micronemes comprise two Rab5A/C-dependence-defined sub-populations and identified a ferlin acting differentially on them, answering how distinct microneme cargo is trafficked and exocytosed.\",\n      \"evidence\": \"Dominant-negative overexpression of TgFER1 with live imaging and microneme exocytosis assays in T. gondii\",\n      \"pmids\": [\"39294204\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Dominant-negative rather than clean loss-of-function\",\n        \"Mechanism distinguishing Rab5A-dependent vs -independent microneme sub-populations unknown\",\n        \"Direct Rab5A-FER1 coupling not established\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The direct effectors of activated TgRab5A and the structural basis of its nucleotide switching remain unidentified.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct Rab5A effector or interactor mapped biochemically\",\n        \"No structural model of TgRab5A\",\n        \"GTP/GDP cycling of Rab5A in vivo not directly tracked\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003924\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005768\", \"supporting_discovery_ids\": [3], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006886\", \"supporting_discovery_ids\": [0, 1, 3, 4], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0007033\", \"supporting_discovery_ids\": [2, 3], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"TgVps9 (GEF activating Rab5A)\",\n      \"TgVps11 (HOPS/CORVET tethering subunit, downstream)\",\n      \"TgAP1 (clathrin adaptor at Rab5A-positive compartment)\",\n      \"TgFER1 (ferlin acting on Rab5A/C-dependent micronemes)\"\n    ],\n    \"other_free_text\": [\n      \"Regulates trafficking of a subset of microneme and rhoptry proteins from the TGN to secretory organelles; redundant Rab5A-independent microneme targeting pathways exist.\",\n      \"Rab5A/C dependence distinguishes two biogenetically distinct microneme sub-populations.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}\n```","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_267810","name":"RAB5A","product":"ras-related protein RAB5A","uniprot_acc":"A0A125YT53","chromosome":"TGME49_chrIX","start_pos":338653},{"strain":"GT1","gene_id":"TGGT1_267810","name":null,"product":"Rab 5","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":174026},{"strain":"RH88","gene_id":"TGRH88_010300","name":null,"product":"Rab 5","uniprot_acc":null,"chromosome":"CM023090","start_pos":389205},{"strain":"VEG","gene_id":"TGVEG_267810","name":null,"product":"Rab 5","uniprot_acc":"B9QP57","chromosome":"TGVEG_chrIX","start_pos":365910}]}