{"gene":"TG_267620","gene_symbol":"ISC6","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2016,"finding":"ISC6 was identified as a component of the Toxoplasma IMC sutures through proximity-dependent biotin identification (BioID) using ISC4 as bait, establishing it as a new IMC sutures component (ISC) that localizes to the transverse and longitudinal sutures of the inner membrane complex.","method":"Proximity-dependent biotin identification (BioID) with ISC4 as bait, followed by localization studies","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID proximity labeling with fluorescence localization, single lab, single study","pmids":["27696623"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"ISC6 is a component of the Toxoplasma gondii inner membrane complex (IMC) sutures, localizing to both the transverse and longitudinal sutures of this organelle, as identified by proximity-dependent biotin labeling."},"narrative":{"mechanistic_narrative":"ISC6 is a component of the inner membrane complex (IMC) sutures of Toxoplasma gondii, localizing to both the transverse and longitudinal sutures of this organelle, where it was identified as a new IMC sutures component (ISC) by proximity-dependent biotin labeling using ISC4 as bait [PMID:27696623]. Beyond this localization within the IMC suture system, no further mechanistic detail about ISC6 has been characterized in the available corpus.","teleology":[{"year":2016,"claim":"To define the protein composition of the Toxoplasma IMC sutures, ISC6 was sought as a suture-associated protein; its identification established a previously unknown component localizing to both transverse and longitudinal sutures.","evidence":"BioID proximity-dependent biotin labeling with ISC4 as bait followed by fluorescence localization in Toxoplasma gondii","pmids":["27696623"],"confidence":"Medium","gaps":["Molecular function and any enzymatic or structural activity of ISC6 are undefined","Direct binding partners beyond proximity to the ISC4 bait are not established","Contribution of ISC6 to IMC integrity, cytokinesis, or parasite fitness has not been tested"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["IMC sutures component (ISC) system"],"partners":["ISC4 (BIOID BAIT IN PROXIMITY TO ISC6)"],"other_free_text":[]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"27696623","id":"PMC_27696623","title":"Novel insights into the composition and function of the Toxoplasma IMC sutures.","date":"2016","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/27696623","citation_count":59,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2890,"output_tokens":329,"usd":0.006803,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4899,"output_tokens":911,"usd":0.023635,"stage2_stop_reason":"end_turn"},"total_usd":0.032025,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2016,\n      \"finding\": \"ISC6 was identified as a component of the Toxoplasma IMC sutures through proximity-dependent biotin identification (BioID) using ISC4 as bait, establishing it as a new IMC sutures component (ISC) that localizes to the transverse and longitudinal sutures of the inner membrane complex.\",\n      \"method\": \"Proximity-dependent biotin identification (BioID) with ISC4 as bait, followed by localization studies\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID proximity labeling with fluorescence localization, single lab, single study\",\n      \"pmids\": [\"27696623\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"ISC6 is a component of the Toxoplasma gondii inner membrane complex (IMC) sutures, localizing to both the transverse and longitudinal sutures of this organelle, as identified by proximity-dependent biotin labeling.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"ISC6 is a component of the inner membrane complex (IMC) sutures of Toxoplasma gondii, localizing to both the transverse and longitudinal sutures of this organelle, where it was identified as a new IMC sutures component (ISC) by proximity-dependent biotin labeling using ISC4 as bait [#0]. Beyond this localization within the IMC suture system, no further mechanistic detail about ISC6 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2016,\n      \"claim\": \"To define the protein composition of the Toxoplasma IMC sutures, ISC6 was sought as a suture-associated protein; its identification established a previously unknown component localizing to both transverse and longitudinal sutures.\",\n      \"evidence\": \"BioID proximity-dependent biotin labeling with ISC4 as bait followed by fluorescence localization in Toxoplasma gondii\",\n      \"pmids\": [\"27696623\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Molecular function and any enzymatic or structural activity of ISC6 are undefined\",\n        \"Direct binding partners beyond proximity to the ISC4 bait are not established\",\n        \"Contribution of ISC6 to IMC integrity, cytokinesis, or parasite fitness has not been tested\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How ISC6 is targeted to the sutures, what it binds, and what role it plays in IMC biogenesis or division remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or knockout characterization of ISC6\",\n        \"No structural model or membrane-topology data\",\n        \"Recruitment mechanism to the sutures unresolved\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"inner membrane complex sutures (transverse and longitudinal)\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"IMC sutures component (ISC) system\"],\n    \"partners\": [\"ISC4 (BioID bait in proximity to ISC6)\"],\n    \"other_free_text\": []\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_267620","name":"ISC6","product":"IMC sutures component ISC6","uniprot_acc":"A0A125YH16","chromosome":"TGME49_chrIX","start_pos":511479},{"strain":"GT1","gene_id":"TGGT1_267620","name":null,"product":"multi-pass transmembrane protein","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":346132},{"strain":"RH88","gene_id":"TGRH88_010490","name":null,"product":"multi-pass transmembrane protein","uniprot_acc":null,"chromosome":"CM023090","start_pos":561267},{"strain":"VEG","gene_id":"TGVEG_267620","name":null,"product":"multi-pass transmembrane protein","uniprot_acc":"A0A125YH19","chromosome":"TGVEG_chrIX","start_pos":537848}]}