{"gene":"TG_264990","gene_symbol":"IMC16","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2014,"finding":"IMC16 was identified as a protein constituent of the Toxoplasma gondii inner membrane complex (IMC) through epitope tagging of cyclically expressed unknown genes in tachyzoites.","method":"Epitope tagging and localization of representative cyclical unknown genes in T. gondii tachyzoites","journal":"PloS one","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — direct localization experiment (epitope tag) identifying IMC membership, but part of a large screen with limited mechanistic follow-up for IMC16 specifically","pmids":["24841368"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC16 is a constituent protein of the Toxoplasma gondii inner membrane complex (IMC), identified through epitope-tag localization in tachyzoites, with its mRNA cycling in concert with cell division, but its precise molecular function within the IMC remains uncharacterized."},"narrative":{"mechanistic_narrative":"IMC16 is a constituent protein of the Toxoplasma gondii inner membrane complex (IMC), identified through epitope tagging of cyclically expressed unknown genes in tachyzoites, placing it among the cyclically expressed components of this cytoskeletal membrane system [PMID:24841368]. Beyond this localization to the IMC, no further mechanistic detail about IMC16 has been characterized in the available corpus.","teleology":[{"year":2014,"claim":"Whether the cyclically expressed unknown gene IMC16 encoded a functional constituent of a defined subcellular structure was unknown; epitope tagging established it as a component of the Toxoplasma inner membrane complex.","evidence":"Epitope tagging and localization of representative cyclical unknown genes in T. gondii tachyzoites","pmids":["24841368"],"confidence":"Medium","gaps":["The molecular function of IMC16 within the IMC is undefined","No binding partners or protein interactions have been mapped","The role of its cyclical expression in cell division is not mechanistically established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["inner membrane complex (IMC)"],"partners":[],"other_free_text":["mRNA cyclically expressed in concert with cell division"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"24841368","id":"PMC_24841368","title":"Identifying novel cell cycle proteins in Apicomplexa parasites through co-expression decision analysis.","date":"2014","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/24841368","citation_count":15,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2904,"output_tokens":285,"usd":0.006494,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4851,"output_tokens":877,"usd":0.02309,"stage2_stop_reason":"end_turn"},"total_usd":0.031451,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2014,\n      \"finding\": \"IMC16 was identified as a protein constituent of the Toxoplasma gondii inner membrane complex (IMC) through epitope tagging of cyclically expressed unknown genes in tachyzoites.\",\n      \"method\": \"Epitope tagging and localization of representative cyclical unknown genes in T. gondii tachyzoites\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — direct localization experiment (epitope tag) identifying IMC membership, but part of a large screen with limited mechanistic follow-up for IMC16 specifically\",\n      \"pmids\": [\"24841368\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC16 is a constituent protein of the Toxoplasma gondii inner membrane complex (IMC), identified through epitope-tag localization in tachyzoites, with its mRNA cycling in concert with cell division, but its precise molecular function within the IMC remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC16 is a constituent protein of the Toxoplasma gondii inner membrane complex (IMC), identified through epitope tagging of cyclically expressed unknown genes in tachyzoites, placing it among the cyclically expressed components of this cytoskeletal membrane system [#0]. Beyond this localization to the IMC, no further mechanistic detail about IMC16 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Whether the cyclically expressed unknown gene IMC16 encoded a functional constituent of a defined subcellular structure was unknown; epitope tagging established it as a component of the Toxoplasma inner membrane complex.\",\n      \"evidence\": \"Epitope tagging and localization of representative cyclical unknown genes in T. gondii tachyzoites\",\n      \"pmids\": [\n        \"24841368\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"The molecular function of IMC16 within the IMC is undefined\",\n        \"No binding partners or protein interactions have been mapped\",\n        \"The role of its cyclical expression in cell division is not mechanistically established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The precise molecular role of IMC16 within the inner membrane complex, its interaction partners, and its contribution to tachyzoite division remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional or genetic knockout characterization\",\n        \"No structural information or partner identification\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"inner membrane complex\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"mRNA cyclically expressed in concert with cell division\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_264990","name":"IMC16","product":"inner membrane complex protein IMC16","uniprot_acc":"A0A125YMG4","chromosome":"TGME49_chrIX","start_pos":1861490},{"strain":"GT1","gene_id":"TGGT1_264990","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":1680342},{"strain":"RH88","gene_id":"TGRH88_012490","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023090","start_pos":1918992},{"strain":"VEG","gene_id":"TGVEG_264990","name":null,"product":"hypothetical protein","uniprot_acc":"B9Q1J0","chromosome":"TGVEG_chrIX","start_pos":1889994}]}