{"gene":"TG_262690","gene_symbol":"RPL27","run_date":"2026-09-08T14:08:29","timeline":{"discoveries":[{"year":2017,"finding":"RPL27 (along with translation elongation factor 1α and RPS18) was identified as a potential binding protein of the antiparasitic trithiolato arene ruthenium complex 9 in T. gondii, via affinity chromatography coupled to Sepharose and mass spectrometry.","method":"Affinity chromatography (compound coupled to epoxy-activated Sepharose) followed by mass spectrometry","journal":"Antimicrobial agents and chemotherapy","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single affinity pulldown without functional validation or mutagenesis; binding was inferred from MS identification, not confirmed by reciprocal or orthogonal method","pmids":["28652238"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"T. gondii RPL27 is a ribosomal protein that was identified as a candidate binding target of an antiparasitic ruthenium complex by affinity chromatography and mass spectrometry, but no further mechanistic characterization of its molecular function has been reported in the available literature."},"narrative":{"mechanistic_narrative":"RPL27 is a Toxoplasma gondii ribosomal protein identified as a candidate binding target of the antiparasitic trithiolato arene ruthenium complex 9, recovered by affinity chromatography and mass spectrometry alongside translation elongation factor 1α and RPS18 [PMID:28652238]. Beyond this single affinity-based identification, no molecular function, structural detail, or functional validation of RPL27 has been characterized in the available corpus.","teleology":[{"year":2017,"claim":"To identify the parasite targets of an antiparasitic ruthenium complex, affinity capture was used, placing RPL27 among candidate binding proteins and implicating the translation machinery as a possible drug interaction site.","evidence":"Affinity chromatography with compound-coupled Sepharose followed by mass spectrometry in T. gondii","pmids":["28652238"],"confidence":"Low","gaps":["Binding inferred from MS identification only, not confirmed by reciprocal or orthogonal method","No functional validation or mutagenesis linking RPL27 to compound activity","No characterization of RPL27's role in ribosome function or translation"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"Whether RPL27 is a genuine functional target of the ruthenium complex, and what its molecular role in the parasite ribosome and translation is, remains unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["No orthogonal confirmation of the RPL27-compound interaction","No structural or biochemical characterization of RPL27","No genetic or phenotypic evidence of RPL27 function"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["TRITHIOLATO ARENE RUTHENIUM COMPLEX 9 (CANDIDATE BINDING TARGET, [#0])"],"other_free_text":["Identified as a ribosomal protein co-recovered with translation elongation factor 1α and RPS18 in an affinity pulldown [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"28652238","id":"PMC_28652238","title":"Characterization of the Activities of Dinuclear Thiolato-Bridged Arene Ruthenium Complexes against Toxoplasma gondii.","date":"2017","source":"Antimicrobial agents and chemotherapy","url":"https://pubmed.ncbi.nlm.nih.gov/28652238","citation_count":28,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3008,"output_tokens":339,"usd":0.007055,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4927,"output_tokens":896,"usd":0.023518,"stage2_stop_reason":"end_turn"},"total_usd":0.030573,"stage1_batch_id":"msgbatch_015xmQKDRqnTh9anE4m7LiD6","stage2_batch_id":"msgbatch_01JLpkFTVhoQBNgqHCKFrVVU","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2017,\n      \"finding\": \"RPL27 (along with translation elongation factor 1α and RPS18) was identified as a potential binding protein of the antiparasitic trithiolato arene ruthenium complex 9 in T. gondii, via affinity chromatography coupled to Sepharose and mass spectrometry.\",\n      \"method\": \"Affinity chromatography (compound coupled to epoxy-activated Sepharose) followed by mass spectrometry\",\n      \"journal\": \"Antimicrobial agents and chemotherapy\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single affinity pulldown without functional validation or mutagenesis; binding was inferred from MS identification, not confirmed by reciprocal or orthogonal method\",\n      \"pmids\": [\"28652238\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"T. gondii RPL27 is a ribosomal protein that was identified as a candidate binding target of an antiparasitic ruthenium complex by affinity chromatography and mass spectrometry, but no further mechanistic characterization of its molecular function has been reported in the available literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"RPL27 is a Toxoplasma gondii ribosomal protein identified as a candidate binding target of the antiparasitic trithiolato arene ruthenium complex 9, recovered by affinity chromatography and mass spectrometry alongside translation elongation factor 1α and RPS18 [#0]. Beyond this single affinity-based identification, no molecular function, structural detail, or functional validation of RPL27 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2017,\n      \"claim\": \"To identify the parasite targets of an antiparasitic ruthenium complex, affinity capture was used, placing RPL27 among candidate binding proteins and implicating the translation machinery as a possible drug interaction site.\",\n      \"evidence\": \"Affinity chromatography with compound-coupled Sepharose followed by mass spectrometry in T. gondii\",\n      \"pmids\": [\n        \"28652238\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Binding inferred from MS identification only, not confirmed by reciprocal or orthogonal method\",\n        \"No functional validation or mutagenesis linking RPL27 to compound activity\",\n        \"No characterization of RPL27's role in ribosome function or translation\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"Whether RPL27 is a genuine functional target of the ruthenium complex, and what its molecular role in the parasite ribosome and translation is, remains unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No orthogonal confirmation of the RPL27-compound interaction\",\n        \"No structural or biochemical characterization of RPL27\",\n        \"No genetic or phenotypic evidence of RPL27 function\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\n      \"Trithiolato arene ruthenium complex 9 (candidate binding target, [#0])\"\n    ],\n    \"other_free_text\": [\n      \"Identified as a ribosomal protein co-recovered with translation elongation factor 1α and RPS18 in an affinity pulldown [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_262690","name":"RPL27","product":"ribosomal protein RPL27","uniprot_acc":"S8F1P5","chromosome":"TGME49_chrVIIb","start_pos":1080014},{"strain":"GT1","gene_id":"TGGT1_262690","name":null,"product":"ribosomal protein RPL27","uniprot_acc":null,"chromosome":"TGGT1_chrVIIb","start_pos":1055302},{"strain":"RH88","gene_id":"TGRH88_068330","name":null,"product":"ribosomal protein RPL27","uniprot_acc":null,"chromosome":"CM023089","start_pos":1095169},{"strain":"VEG","gene_id":"TGVEG_262690","name":null,"product":"ribosomal protein RPL27","uniprot_acc":"A0A125YU34","chromosome":"TGVEG_chrVIIb","start_pos":1089459}]}