{"gene":"TG_260180","gene_symbol":"ICAP6","run_date":"2026-09-08T12:58:25","timeline":{"discoveries":[],"current_model":"No mechanistic findings in the available literature."},"narrative":{"mechanistic_narrative":"No mechanistic discoveries found in literature.","teleology":[],"mechanism_profile":null,"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"8579614","id":"PMC_8579614","title":"Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).","date":"1996","source":"Biochemical and biophysical research communications","url":"https://pubmed.ncbi.nlm.nih.gov/8579614","citation_count":263,"is_preprint":false},{"pmid":"25879281","id":"PMC_25879281","title":"The NLRP1 inflammasomes.","date":"2015","source":"Immunological reviews","url":"https://pubmed.ncbi.nlm.nih.gov/25879281","citation_count":200,"is_preprint":false},{"pmid":"30204085","id":"PMC_30204085","title":"Identification of cryptic subunits from an apicomplexan ATP synthase.","date":"2018","source":"eLife","url":"https://pubmed.ncbi.nlm.nih.gov/30204085","citation_count":53,"is_preprint":false},{"pmid":"30685461","id":"PMC_30685461","title":"Drug Resistance in Protozoan Parasites: An Incessant Wrestle for Survival.","date":"2019","source":"Journal of global antimicrobial resistance","url":"https://pubmed.ncbi.nlm.nih.gov/30685461","citation_count":37,"is_preprint":false},{"pmid":"31091451","id":"PMC_31091451","title":"The Toxoplasma Vacuolar H+-ATPase Regulates Intracellular pH and Impacts the Maturation of Essential Secretory Proteins.","date":"2019","source":"Cell reports","url":"https://pubmed.ncbi.nlm.nih.gov/31091451","citation_count":35,"is_preprint":false},{"pmid":"10514077","id":"PMC_10514077","title":"Recombinant expression, purification, and characterization of Toxoplasma gondii adenosine kinase.","date":"1999","source":"Molecular and biochemical parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/10514077","citation_count":35,"is_preprint":false},{"pmid":"21315696","id":"PMC_21315696","title":"Induction of protective Th1 immune responses in mice by vaccination with recombinant Toxoplasma gondii nucleoside triphosphate hydrolase-II.","date":"2011","source":"Vaccine","url":"https://pubmed.ncbi.nlm.nih.gov/21315696","citation_count":35,"is_preprint":false},{"pmid":"27559003","id":"PMC_27559003","title":"P2X7 receptor-dependent tuning of gut epithelial responses to infection.","date":"2016","source":"Immunology and cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/27559003","citation_count":33,"is_preprint":false},{"pmid":"30599391","id":"PMC_30599391","title":"New life for an old drug: In vitro and in vivo effects of the anthelmintic drug niclosamide against Toxoplasma gondii RH strain.","date":"2018","source":"International journal for parasitology. Drugs and drug resistance","url":"https://pubmed.ncbi.nlm.nih.gov/30599391","citation_count":33,"is_preprint":false},{"pmid":"20487267","id":"PMC_20487267","title":"Novel roles for ATP-binding cassette G transporters in lipid redistribution in Toxoplasma.","date":"2010","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/20487267","citation_count":32,"is_preprint":false},{"pmid":"28069296","id":"PMC_28069296","title":"Inflammatory early events associated to the role of P2X7 receptor in acute murine toxoplasmosis.","date":"2016","source":"Immunobiology","url":"https://pubmed.ncbi.nlm.nih.gov/28069296","citation_count":32,"is_preprint":false},{"pmid":"36302692","id":"PMC_36302692","title":"The mystery of massive mitochondrial complexes: the apicomplexan respiratory chain.","date":"2022","source":"Trends in parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/36302692","citation_count":30,"is_preprint":false},{"pmid":"25389034","id":"PMC_25389034","title":"Intestinal immunopathology is associated with decreased CD73-generated adenosine during lethal infection.","date":"2014","source":"Mucosal immunology","url":"https://pubmed.ncbi.nlm.nih.gov/25389034","citation_count":30,"is_preprint":false},{"pmid":"21799871","id":"PMC_21799871","title":"Identification of Toxoplasma gondii cAMP dependent protein kinase and its role in the tachyzoite growth.","date":"2011","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/21799871","citation_count":30,"is_preprint":false},{"pmid":"10470019","id":"PMC_10470019","title":"Characterization of myosin-A and myosin-C: two class XIV unconventional myosins from Toxoplasma gondii.","date":"1999","source":"Cell motility and the cytoskeleton","url":"https://pubmed.ncbi.nlm.nih.gov/10470019","citation_count":29,"is_preprint":false},{"pmid":"31914409","id":"PMC_31914409","title":"Characterization of the apicoplast-localized enzyme TgUroD in Toxoplasma gondii reveals a key role of the apicoplast in heme biosynthesis.","date":"2019","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/31914409","citation_count":28,"is_preprint":false},{"pmid":"15083530","id":"PMC_15083530","title":"Actin and myosin in Gregarina polymorpha.","date":"2004","source":"Cell motility and the cytoskeleton","url":"https://pubmed.ncbi.nlm.nih.gov/15083530","citation_count":27,"is_preprint":false},{"pmid":"22130673","id":"PMC_22130673","title":"Structural insight into activation mechanism of Toxoplasma gondii nucleoside triphosphate diphosphohydrolases by disulfide reduction.","date":"2011","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/22130673","citation_count":26,"is_preprint":false},{"pmid":"30824738","id":"PMC_30824738","title":"Mapping a novel positive allosteric modulator binding site in the central vestibule region of human P2X7.","date":"2019","source":"Scientific 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signalling","url":"https://pubmed.ncbi.nlm.nih.gov/28815408","citation_count":24,"is_preprint":false},{"pmid":"30429052","id":"PMC_30429052","title":"P2X7 receptor-mediated leukocyte recruitment and Porphyromonas gingivalis clearance requires IL-1β production and autocrine IL-1 receptor activation.","date":"2018","source":"Immunobiology","url":"https://pubmed.ncbi.nlm.nih.gov/30429052","citation_count":23,"is_preprint":false},{"pmid":"41789725","id":"PMC_41789725","title":"Unconventional model organisms bend our view on mitochondrial cristae.","date":"2026","source":"Journal of cell science","url":"https://pubmed.ncbi.nlm.nih.gov/41789725","citation_count":0,"is_preprint":false},{"pmid":"42313860","id":"PMC_42313860","title":"Inhibition of Toxoplasma gondii proliferation by dimethyl itaconate: Evidence from in vitro and in vivo studies.","date":"2026","source":"PLoS neglected tropical diseases","url":"https://pubmed.ncbi.nlm.nih.gov/42313860","citation_count":0,"is_preprint":false},{"pmid":"42124602","id":"PMC_42124602","title":"A two-membrane gateway for monocarboxylates couples host glycolysis to Toxoplasma mitochondrial fitness and virulence.","date":"2026","source":"bioRxiv : the preprint server for biology","url":"https://pubmed.ncbi.nlm.nih.gov/42124602","citation_count":0,"is_preprint":false},{"pmid":"41843302","id":"PMC_41843302","title":"Estudo Abrangente do Resveratrol Associado à Sulfadiazina em Linfócitos Infectados com Toxoplasma gondii: Envolvimento da Sinalização Purinérgica na Resposta Imunológica.","date":"2026","source":"Acta parasitologica","url":"https://pubmed.ncbi.nlm.nih.gov/41843302","citation_count":0,"is_preprint":false},{"pmid":"40407244","id":"PMC_40407244","title":"Bumped Kinase Inhibitors Inhibit both Toxoplasma gondii MAPKL1 and CDPK1.","date":"2025","source":"ACS infectious diseases","url":"https://pubmed.ncbi.nlm.nih.gov/40407244","citation_count":7,"is_preprint":false},{"pmid":"40217127","id":"PMC_40217127","title":"An ISWI-related chromatin remodeller regulates stage-specific gene expression in Toxoplasma gondii.","date":"2025","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/40217127","citation_count":7,"is_preprint":false},{"pmid":"40556512","id":"PMC_40556512","title":"Toxoplasma gondii GRA16 Suppresses Aerobic Glycolysis by Downregulating c-Myc and TERT Expressions in Colorectal Cancer Cells.","date":"2025","source":"Biomolecules & therapeutics","url":"https://pubmed.ncbi.nlm.nih.gov/40556512","citation_count":4,"is_preprint":false},{"pmid":"40576341","id":"PMC_40576341","title":"The critical role of Toxoplasma gondii GRA1 in nutrient salvage.","date":"2025","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/40576341","citation_count":3,"is_preprint":false},{"pmid":"40001505","id":"PMC_40001505","title":"Activity Evaluation and Mode of Action of ICA Against Toxoplasma gondii In Vitro.","date":"2025","source":"Biomolecules","url":"https://pubmed.ncbi.nlm.nih.gov/40001505","citation_count":3,"is_preprint":false},{"pmid":"41427835","id":"PMC_41427835","title":"PI3K/Akt/mTOR signaling pathway mediates energy metabolic reprogramming and regulates mitochondrial homeostasis in host cells exposed to Toxoplasma gondii.","date":"2025","source":"Microbiology spectrum","url":"https://pubmed.ncbi.nlm.nih.gov/41427835","citation_count":2,"is_preprint":false},{"pmid":"40572091","id":"PMC_40572091","title":"Piceatannol Induces Mitochondrial Dysfunction in Toxoplasma gondii.","date":"2025","source":"Microorganisms","url":"https://pubmed.ncbi.nlm.nih.gov/40572091","citation_count":2,"is_preprint":false},{"pmid":"40731026","id":"PMC_40731026","title":"Proline dehydrogenase, a rate-limiting catabolic enzyme, affecting the growth and pathogenicity of Toxoplasma gondii tachyzoites by regulating the proline metabolism and mitochondrial function of the parasite.","date":"2025","source":"Parasites & vectors","url":"https://pubmed.ncbi.nlm.nih.gov/40731026","citation_count":1,"is_preprint":false},{"pmid":"40723844","id":"PMC_40723844","title":"Elucidating the Role of Toxoplasma gondii's Mitochondrial Superoxide Dismutase.","date":"2025","source":"Biomolecules","url":"https://pubmed.ncbi.nlm.nih.gov/40723844","citation_count":1,"is_preprint":false},{"pmid":"41193440","id":"PMC_41193440","title":"Structural and functional characterization of TgGSK3, a druggable kinase in Toxoplasma gondii.","date":"2025","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/41193440","citation_count":0,"is_preprint":false},{"pmid":"40830525","id":"PMC_40830525","title":"Cross-lineage 5-methylcytosine methylome profiling reveals methylated divergence among Toxoplasma gondii tachyzoites of the three major clonal lineages.","date":"2025","source":"Infectious diseases of poverty","url":"https://pubmed.ncbi.nlm.nih.gov/40830525","citation_count":0,"is_preprint":false},{"pmid":"41441052","id":"PMC_41441052","title":"Impacts of Cipargamin on Na+-ATPase and Osmoregulation of Trypanosoma cruzi.","date":"2025","source":"Membranes","url":"https://pubmed.ncbi.nlm.nih.gov/41441052","citation_count":0,"is_preprint":false},{"pmid":"39878652","id":"PMC_39878652","title":"TgGloL is an atypical glyoxalase/VOC domain-containing apicoplast protein that is important for the growth of Toxoplasma.","date":"2025","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/39878652","citation_count":0,"is_preprint":false},{"pmid":"38589375","id":"PMC_38589375","title":"Metabolic plasticity, essentiality and therapeutic potential of ribose-5-phosphate synthesis in Toxoplasma gondii.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/38589375","citation_count":11,"is_preprint":false},{"pmid":"39375464","id":"PMC_39375464","title":"Recognition of phylogenetically diverse pathogens through enzymatically amplified recruitment of RNF213.","date":"2024","source":"EMBO reports","url":"https://pubmed.ncbi.nlm.nih.gov/39375464","citation_count":11,"is_preprint":false},{"pmid":"39207139","id":"PMC_39207139","title":"The Toxoplasma gondii mitochondrial transporter ABCB7L is essential for the biogenesis of cytosolic and nuclear iron-sulfur cluster proteins and cytosolic translation.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/39207139","citation_count":9,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":23999,"output_tokens":352,"usd":0.038638,"stage1_stop_reason":"end_turn"},"stage2":{"model":"","input_tokens":0,"output_tokens":0,"usd":0.0},"total_usd":0.038638,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard"},"stage1_raw":"Looking at the corpus, I need to identify papers about ICAP6, a Toxoplasma gondii gene. Based on the corpus context, ICAP6 appears to be one of the novel ATP synthase subunits identified in Toxoplasma gondii (the paper about \"cryptic subunits from an apicomplexan ATP synthase\" [PMID:30204085] mentions ICAP2 and ICAP18, suggesting ICAP6 is part of the same family of novel apicomplexan-specific ATP synthase subunits).\n\nHowever, scanning all 43 abstracts carefully:\n- Paper [3] (PMID:30204085) discusses ICAP2 and ICAP18, not ICAP6\n- Paper [12] (PMID:36302692) discusses apicomplexan respiratory chain broadly\n- Paper [24] (PMID:41789725) discusses ATP synthase and cristae broadly\n\nNone of the abstracts specifically describe experimental findings about ICAP6. The corpus does not contain any abstract that names ICAP6 specifically or establishes mechanistic findings about it. The most relevant paper [3] establishes findings about ICAP2 and ICAP18, not ICAP6.\n\nSince I cannot ground any discovery about ICAP6 specifically in any abstract from the corpus, I must return an empty discoveries list.\n\n```json\n{\n  \"discoveries\": [],\n  \"current_model\": \"No mechanistic findings in the available literature.\"\n}\n```","stage2_raw":"","audit_flag":{"gene":"TG_260180","tier":"COVERAGE","verdict":"Coverage note","subtype":"corpus_generic","uniprot_band":"empty","rules_fired":"R1","issue":"R1: gene named in 0/100 (0%) of its corpus abstracts and Stage 1 extracted no discoveries — no gene-specific literature retrieved"},"strains":[{"strain":"ME49","gene_id":"TGME49_260180","name":"ICAP6","product":"ATP synthase-associated protein","uniprot_acc":"A0A125YTD8","chromosome":"TGME49_chrVIIb","start_pos":2475050},{"strain":"GT1","gene_id":"TGGT1_260180","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIIb","start_pos":2449773},{"strain":"RH88","gene_id":"TGRH88_070180","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":2495475},{"strain":"VEG","gene_id":"TGVEG_260180","name":null,"product":"hypothetical protein","uniprot_acc":"B9PKR3","chromosome":"TGVEG_chrVIIb","start_pos":2491119}]}