{"gene":"TG_259630","gene_symbol":"IMC27","run_date":"2026-09-08T15:31:43+00:00","timeline":{"discoveries":[{"year":2016,"finding":"IMC27 was identified as a component of the Toxoplasma IMC sutures through proximity-dependent biotin identification (BioID) using ISC4 as bait, placing it within the IMC suture subcompartment.","method":"Proximity-dependent biotin identification (BioID) / proximity labeling proteomics","journal":"Cellular microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID proximity labeling with a single bait protein in a single lab; IMC27 identified as a labeled protein but no direct functional follow-up on IMC27 itself reported in this abstract.","pmids":["27696623"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2019,"finding":"IMC27 physically interacts with the essential IMC protein ILP1 via ILP1's C-terminal coiled-coil domain, as captured by photoactivatable unnatural amino acid crosslinking in the native intracellular environment, placing IMC27 within the cytoskeletal protein-interaction network of the IMC.","method":"Photoactivatable unnatural amino acid (UAA) crosslinking followed by mass spectrometry; domain-mapping experiments identifying the ILP1 C-terminal coiled-coil region as the binding interface","journal":"PLoS biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — in-cell photo-crosslinking with domain-level mapping provides direct interaction evidence; single lab but two orthogonal aspects (crosslinking + domain truncations for ILP1 partners IMC3/IMC6 validate the approach), though IMC27-specific follow-up functional data are not reported.","pmids":["31584943"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC27 is a component of the Toxoplasma gondii inner membrane complex (IMC) sutures and physically interacts with the essential cytoskeletal protein ILP1 through ILP1's C-terminal coiled-coil domain, placing IMC27 within the alveolin-based cytoskeletal network of the IMC."},"narrative":{"mechanistic_narrative":"IMC27 is a component of the inner membrane complex (IMC) of Toxoplasma gondii, localizing to the IMC suture subcompartment where it was identified by proximity labeling using the suture protein ISC4 as bait [PMID:27696623]. Within the alveolin-based cytoskeletal network of the IMC, IMC27 physically interacts with the essential IMC protein ILP1 through ILP1's C-terminal coiled-coil domain, as captured by in-cell photo-crosslinking and domain mapping [PMID:31584943]. Beyond this suture localization and ILP1 interaction, no functional role, essentiality, or downstream mechanism for IMC27 itself has been characterized in the available corpus.","teleology":[{"year":2016,"claim":"Before this work the molecular composition of the IMC suture subcompartment was undefined; BioID from the suture bait ISC4 placed IMC27 in this compartment, establishing it as a suture-associated protein.","evidence":"Proximity-dependent biotin identification (BioID) with ISC4 as bait in Toxoplasma gondii","pmids":["27696623"],"confidence":"Medium","gaps":["Single bait in one lab; proximity labeling does not distinguish direct from indirect neighbors","No functional or knockout follow-up on IMC27 itself","Sub-suture localization not resolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2019,"claim":"It was unknown which cytoskeletal partners tether IMC27 within the IMC; in-cell photo-crosslinking and domain mapping showed IMC27 binds the essential protein ILP1 via ILP1's C-terminal coiled-coil, integrating IMC27 into the IMC protein-interaction network.","evidence":"Photoactivatable unnatural amino acid crosslinking with mass spectrometry and ILP1 domain-truncation mapping in the native intracellular environment","pmids":["31584943"],"confidence":"Medium","gaps":["IMC27-specific functional consequence of the interaction not tested","Whether the interaction is direct versus bridged not fully resolved","Region of IMC27 mediating binding not mapped"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The functional role of IMC27 in IMC assembly, stability, or parasite viability remains uncharacterized.","evidence":"No loss-of-function, essentiality, or phenotypic study of IMC27 is present in the corpus","pmids":[],"confidence":"Medium","gaps":["No knockout or knockdown phenotype","No structural model of IMC27","Role in suture formation or daughter cell budding untested"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["IMC alveolin-based cytoskeletal network / suture subcompartment"],"partners":["ILP1 (VIA ILP1 C-TERMINAL COILED-COIL DOMAIN)","ISC4 (PROXIMITY)"],"other_free_text":["Identified as a suture-associated IMC protein by BioID with ISC4 bait [#0].","Interaction with ILP1 captured by in-cell photo-crosslinking; ILP1 C-terminal coiled-coil is the binding interface [#1]."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"27696623","id":"PMC_27696623","title":"Novel insights into the composition and function of the Toxoplasma IMC sutures.","date":"2016","source":"Cellular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/27696623","citation_count":59,"is_preprint":false},{"pmid":"31584943","id":"PMC_31584943","title":"A photoactivatable crosslinking system reveals protein interactions in the Toxoplasma gondii inner membrane complex.","date":"2019","source":"PLoS biology","url":"https://pubmed.ncbi.nlm.nih.gov/31584943","citation_count":33,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3332,"output_tokens":630,"usd":0.009723,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5357,"output_tokens":1463,"usd":0.03168,"stage2_stop_reason":"end_turn"},"total_usd":0.0,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2016,\n      \"finding\": \"IMC27 was identified as a component of the Toxoplasma IMC sutures through proximity-dependent biotin identification (BioID) using ISC4 as bait, placing it within the IMC suture subcompartment.\",\n      \"method\": \"Proximity-dependent biotin identification (BioID) / proximity labeling proteomics\",\n      \"journal\": \"Cellular microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID proximity labeling with a single bait protein in a single lab; IMC27 identified as a labeled protein but no direct functional follow-up on IMC27 itself reported in this abstract.\",\n      \"pmids\": [\"27696623\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"finding\": \"IMC27 physically interacts with the essential IMC protein ILP1 via ILP1's C-terminal coiled-coil domain, as captured by photoactivatable unnatural amino acid crosslinking in the native intracellular environment, placing IMC27 within the cytoskeletal protein-interaction network of the IMC.\",\n      \"method\": \"Photoactivatable unnatural amino acid (UAA) crosslinking followed by mass spectrometry; domain-mapping experiments identifying the ILP1 C-terminal coiled-coil region as the binding interface\",\n      \"journal\": \"PLoS biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — in-cell photo-crosslinking with domain-level mapping provides direct interaction evidence; single lab but two orthogonal aspects (crosslinking + domain truncations for ILP1 partners IMC3/IMC6 validate the approach), though IMC27-specific follow-up functional data are not reported.\",\n      \"pmids\": [\"31584943\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"IMC27 is a component of the Toxoplasma gondii inner membrane complex (IMC) sutures and physically interacts with the essential cytoskeletal protein ILP1 through ILP1's C-terminal coiled-coil domain, placing IMC27 within the alveolin-based cytoskeletal network of the IMC.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC27 is a component of the inner membrane complex (IMC) of Toxoplasma gondii, localizing to the IMC suture subcompartment where it was identified by proximity labeling using the suture protein ISC4 as bait [#0]. Within the alveolin-based cytoskeletal network of the IMC, IMC27 physically interacts with the essential IMC protein ILP1 through ILP1's C-terminal coiled-coil domain, as captured by in-cell photo-crosslinking and domain mapping [#1]. Beyond this suture localization and ILP1 interaction, no functional role, essentiality, or downstream mechanism for IMC27 itself has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2016,\n      \"claim\": \"Before this work the molecular composition of the IMC suture subcompartment was undefined; BioID from the suture bait ISC4 placed IMC27 in this compartment, establishing it as a suture-associated protein.\",\n      \"evidence\": \"Proximity-dependent biotin identification (BioID) with ISC4 as bait in Toxoplasma gondii\",\n      \"pmids\": [\"27696623\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Single bait in one lab; proximity labeling does not distinguish direct from indirect neighbors\",\n        \"No functional or knockout follow-up on IMC27 itself\",\n        \"Sub-suture localization not resolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2019,\n      \"claim\": \"It was unknown which cytoskeletal partners tether IMC27 within the IMC; in-cell photo-crosslinking and domain mapping showed IMC27 binds the essential protein ILP1 via ILP1's C-terminal coiled-coil, integrating IMC27 into the IMC protein-interaction network.\",\n      \"evidence\": \"Photoactivatable unnatural amino acid crosslinking with mass spectrometry and ILP1 domain-truncation mapping in the native intracellular environment\",\n      \"pmids\": [\"31584943\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"IMC27-specific functional consequence of the interaction not tested\",\n        \"Whether the interaction is direct versus bridged not fully resolved\",\n        \"Region of IMC27 mediating binding not mapped\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The functional role of IMC27 in IMC assembly, stability, or parasite viability remains uncharacterized.\",\n      \"evidence\": \"No loss-of-function, essentiality, or phenotypic study of IMC27 is present in the corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No knockout or knockdown phenotype\",\n        \"No structural model of IMC27\",\n        \"Role in suture formation or daughter cell budding untested\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0005515\", \"supporting_discovery_ids\": [1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0070258\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [\"IMC alveolin-based cytoskeletal network / suture subcompartment\"],\n    \"partners\": [\"ILP1 (via ILP1 C-terminal coiled-coil domain)\", \"ISC4 (proximity)\"],\n    \"other_free_text\": [\n      \"Identified as a suture-associated IMC protein by BioID with ISC4 bait [#0].\",\n      \"Interaction with ILP1 captured by in-cell photo-crosslinking; ILP1 C-terminal coiled-coil is the binding interface [#1].\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_259630","name":"IMC27","product":"inner membrane complex protein IMC27","uniprot_acc":"S8GC95","chromosome":"TGME49_chrVIIb","start_pos":2748676},{"strain":"GT1","gene_id":"TGGT1_259630","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIIb","start_pos":2722823},{"strain":"RH88","gene_id":"TGRH88_070520","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":2768538},{"strain":"VEG","gene_id":"TGVEG_259630","name":null,"product":"hypothetical protein","uniprot_acc":"A0A0F7V2T4","chromosome":"TGVEG_chrVIIb","start_pos":2766042}]}