{"gene":"TG_255450","gene_symbol":"IMC31","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2023,"finding":"IMC31 was identified as one of eight novel IMC proteins enriched in daughter buds, discovered via proximity labeling anchored to IMC29, significantly expanding the daughter IMC proteome.","method":"Proximity labeling (BioID) with IMC29 as bait, followed by mass spectrometry identification","journal":"mBio","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single identification by proximity labeling with no functional follow-up specific to IMC31; IMC31 is listed as a novel candidate, not individually validated","pmids":["36622147"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC31 is a novel inner membrane complex protein enriched in daughter buds during Toxoplasma gondii replication, identified by proximity labeling, with no further mechanistic characterization reported in the available literature."},"narrative":{"mechanistic_narrative":"IMC31 is a novel inner membrane complex protein of Toxoplasma gondii identified as one of eight IMC proteins enriched in daughter buds during endodyogeny, discovered through proximity labeling anchored to the daughter IMC protein IMC29 [PMID:36622147]. Beyond this identification, nothing further is known about IMC31 in the available corpus.","teleology":[{"year":2023,"claim":"The daughter-bud IMC proteome was incompletely defined; anchoring proximity labeling to IMC29 revealed IMC31 as a novel constituent enriched in forming daughter buds, placing it within the machinery of Toxoplasma replication.","evidence":"BioID proximity labeling using IMC29 as bait followed by mass spectrometry in Toxoplasma gondii","pmids":["36622147"],"confidence":"Low","gaps":["Identification by proximity labeling only, with no functional follow-up specific to IMC31","Precise subcellular localization within the IMC and interaction partners not established","No genetic knockout or phenotype characterizing IMC31's role in daughter bud formation"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["IMC29 (PROXIMITY-LABELING BAIT USED TO IDENTIFY IMC31) [#0]"],"other_free_text":["IMC31 is one of eight novel IMC proteins enriched in daughter buds during Toxoplasma gondii endodyogeny [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"36622147","id":"PMC_36622147","title":"IMC29 Plays an Important Role in Toxoplasma Endodyogeny and Reveals New Components of the Daughter-Enriched IMC Proteome.","date":"2023","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/36622147","citation_count":30,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3105,"output_tokens":284,"usd":0.006788,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4851,"output_tokens":884,"usd":0.023178,"stage2_stop_reason":"end_turn"},"total_usd":0.031448,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC31 was identified as one of eight novel IMC proteins enriched in daughter buds, discovered via proximity labeling anchored to IMC29, significantly expanding the daughter IMC proteome.\",\n      \"method\": \"Proximity labeling (BioID) with IMC29 as bait, followed by mass spectrometry identification\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single identification by proximity labeling with no functional follow-up specific to IMC31; IMC31 is listed as a novel candidate, not individually validated\",\n      \"pmids\": [\"36622147\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC31 is a novel inner membrane complex protein enriched in daughter buds during Toxoplasma gondii replication, identified by proximity labeling, with no further mechanistic characterization reported in the available literature.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC31 is a novel inner membrane complex protein of Toxoplasma gondii identified as one of eight IMC proteins enriched in daughter buds during endodyogeny, discovered through proximity labeling anchored to the daughter IMC protein IMC29 [#0]. Beyond this identification, nothing further is known about IMC31 in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2023,\n      \"claim\": \"The daughter-bud IMC proteome was incompletely defined; anchoring proximity labeling to IMC29 revealed IMC31 as a novel constituent enriched in forming daughter buds, placing it within the machinery of Toxoplasma replication.\",\n      \"evidence\": \"BioID proximity labeling using IMC29 as bait followed by mass spectrometry in Toxoplasma gondii\",\n      \"pmids\": [\n        \"36622147\"\n      ],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Identification by proximity labeling only, with no functional follow-up specific to IMC31\",\n        \"Precise subcellular localization within the IMC and interaction partners not established\",\n        \"No genetic knockout or phenotype characterizing IMC31's role in daughter bud formation\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function, direct interaction partners, and contribution of IMC31 to daughter bud assembly and parasite replication remain uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No functional validation of IMC31\",\n        \"No structural or biochemical characterization\",\n        \"No loss-of-function phenotype reported\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\n      \"IMC29 (proximity-labeling bait used to identify IMC31) [#0]\"\n    ],\n    \"other_free_text\": [\n      \"IMC31 is one of eight novel IMC proteins enriched in daughter buds during Toxoplasma gondii endodyogeny [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_255450","name":"IMC31","product":"inner membrane complex protein IMC31","uniprot_acc":"A0A125YWL6","chromosome":"TGME49_chrVIIb","start_pos":4814644},{"strain":"GT1","gene_id":"TGGT1_255450","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIIb","start_pos":4723734},{"strain":"RH88","gene_id":"TGRH88_073480","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":4849579},{"strain":"VEG","gene_id":"TGVEG_255450","name":null,"product":"hypothetical protein","uniprot_acc":"B6KAN9","chromosome":"TGVEG_chrVIIb","start_pos":4836722}]}