{"gene":"TG_251740","gene_symbol":"AP2XII9","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2024,"finding":"TgAP2XII-9 is a nuclear transcription factor expressed predominantly during the S/M phase of the tachyzoite cell cycle. CUT&Tag experiments showed it directly targets promoters of moving junction machinery genes (RON2, RON4, RON8) and daughter cell inner membrane complex (IMC) genes. Conditional knockdown of TgAP2XII-9 caused significant downregulation of rhoptry proteins and rhoptry neck proteins, leading to severe defects in invasion and egress efficiency, as well as disorders in daughter bud formation and apicoplast inheritance, resulting in failure of cell division and intracellular proliferation.","method":"CUT&Tag, conditional knockdown, invasion/egress assays, transcriptomic analysis","journal":"mBio","confidence":"High","confidence_rationale":"Tier 2 / Moderate — conditional knockdown with defined cellular phenotypes plus CUT&Tag genome-wide binding data linking AP2XII-9 directly to target gene promoters, single lab but multiple orthogonal methods","pmids":["39207100"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"finding":"AP2XII-9 localizes to the nucleus with periodic expression peaking during S/M phase. Conditional knockdown in both type I RH and type II Pru strains demonstrated that AP2XII-9 is essential for proper inner membrane complex formation, apicoplast inheritance, and normal cell division. Transcriptomic analysis showed that AP2XII-9 depletion caused downregulation of tachyzoite growth-related genes and upregulation of bradyzoite-specific genes, indicating AP2XII-9 actively represses bradyzoite differentiation even in the absence of alkaline stress.","method":"Conditional knockdown, transcriptomic analysis, immunofluorescence/localization","journal":"FASEB journal","confidence":"High","confidence_rationale":"Tier 2 / Strong — conditional knockdown with defined phenotypic readouts (IMC, apicoplast, cell division, differentiation) replicated in two strains (RH and Pru) with transcriptomic support","pmids":["40106192"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":"RH"},{"species":"Toxoplasma gondii","strain":"Pru"}]},{"year":2024,"finding":"TgAP2XII-9 regulates daughter cell assembly by controlling transcription of at least 300 gene promoters. CUT&Tag and transcriptomics demonstrated that TgAP2XII-9 represses genes necessary for budding initiation while activating genes required for microneme de novo formation. The AP2 DNA-binding domain of TgAP2XII-9 was shown to be critical for its function via domain mutagenesis experiments. A double mutant of TgAP2XII-9 and TgAP2III-2 showed TgAP2XII-9 has the prominent role in daughter cell assembly.","method":"CUT&Tag, transcriptomics, AP2 domain mutagenesis, double-mutant epistasis","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Moderate — multiple orthogonal methods including CUT&Tag, transcriptomics, domain mutagenesis, and genetic epistasis in a single study","pmids":["39774584"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2010,"finding":"AP2XII-9 (identified as one of two dozen AP2 transcription factors) shows a cyclical protein expression profile in the tachyzoite cell cycle, with a sharp peak of protein expression in the S/M-subtranscriptome timeframe, suggesting the timing of protein delivery may be mediated by AP2 domain promoter recognition specificities.","method":"Cell cycle transcriptome profiling, protein expression timing analysis","journal":"PloS one","confidence":"Low","confidence_rationale":"Tier 3 / Weak — expression profiling only, no direct functional characterization of AP2XII-9 specifically","pmids":["20865045"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"AP2XII-9 (TgAP2XII-9) is a nuclear transcription factor expressed predominantly during the S/M phase of the tachyzoite cell cycle that acts as a key activator of genes required for daughter cell inner membrane complex assembly, moving junction machinery (RON2/4/8), rhoptry proteins, microneme formation, and apicoplast inheritance, while simultaneously repressing bradyzoite differentiation genes; conditional loss of AP2XII-9 causes severe defects in endodyogeny, apicoplast inheritance, invasion, and egress, and triggers aberrant bradyzoite differentiation, establishing AP2XII-9 as an essential S/M-phase regulator that coordinates tachyzoite replication and suppresses developmental stage conversion."},"narrative":{"mechanistic_narrative":"AP2XII-9 (TgAP2XII-9) is a nuclear AP2-family transcription factor that operates as an S/M-phase master regulator of the Toxoplasma gondii tachyzoite cell cycle, coordinating daughter cell formation while suppressing developmental stage conversion [PMID:39207100, PMID:40106192]. Its protein abundance oscillates through the cell cycle, peaking sharply during the S/M-subtranscriptome window [PMID:39207100, PMID:20865045], consistent with a role in delivering a transcriptional program at the point of division. Through its AP2 DNA-binding domain, which is required for function, it directly occupies several hundred gene promoters, activating genes for moving junction machinery (RON2, RON4, RON8), the daughter inner membrane complex, and de novo microneme formation, while repressing genes governing budding initiation [PMID:39207100, PMID:39774584]. Conditional knockdown, replicated in type I RH and type II Pru strains, causes downregulation of rhoptry and rhoptry-neck proteins and of tachyzoite growth genes, producing defective daughter bud formation, disrupted apicoplast inheritance, impaired invasion and egress, and failure of intracellular proliferation [PMID:39207100, PMID:40106192]. Depletion also derepresses bradyzoite-specific genes even without alkaline stress, establishing AP2XII-9 as an active repressor of bradyzoite differentiation [PMID:40106192]. Epistasis with TgAP2III-2 shows AP2XII-9 holds the dominant role in daughter cell assembly [PMID:39774584].","teleology":[{"year":2010,"claim":"Establishing whether AP2XII-9 is cell-cycle regulated was the first step in linking it to a specific replicative window; profiling showed its protein peaks sharply in the S/M phase, implicating it in division-associated transcription.","evidence":"Cell cycle transcriptome and protein expression timing profiling in tachyzoites","pmids":["20865045"],"confidence":"Low","gaps":["Expression profiling only; no functional characterization of AP2XII-9 specifically","No promoter targets or DNA-binding activity demonstrated","No phenotype from perturbation"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"claim":"To determine what AP2XII-9 does mechanistically, genome-wide binding and conditional depletion were combined, showing it is a nuclear S/M-phase factor that directly targets moving junction and IMC promoters and is required for invasion, egress, budding, and apicoplast inheritance.","evidence":"CUT&Tag, conditional knockdown, invasion/egress assays, and transcriptomics","pmids":["39207100"],"confidence":"High","gaps":["Single lab and strain background","Direct versus indirect effects on rhoptry protein levels not fully separated","Co-regulators or chromatin partners not identified"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"claim":"Whether the AP2 domain drives function and how AP2XII-9 ranks among related factors was resolved by domain mutagenesis and epistasis, showing the AP2 DNA-binding domain is critical and that AP2XII-9 dominates daughter cell assembly over TgAP2III-2 by both activating microneme genes and repressing budding-initiation genes across ~300 promoters.","evidence":"CUT&Tag, transcriptomics, AP2 domain mutagenesis, and double-mutant epistasis","pmids":["39774584"],"confidence":"High","gaps":["DNA sequence motif recognized by the AP2 domain not defined","Mechanism distinguishing activated versus repressed targets unresolved","Nature of the AP2XII-9/AP2III-2 functional relationship not mechanistically detailed"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"claim":"Whether AP2XII-9's role extends to controlling developmental fate was addressed by conditional knockdown in two strains, showing depletion downregulates tachyzoite growth genes and upregulates bradyzoite-specific genes even without alkaline stress, establishing AP2XII-9 as an active repressor of stage conversion.","evidence":"Conditional knockdown in RH and Pru strains, transcriptomics, and immunofluorescence localization","pmids":["40106192"],"confidence":"High","gaps":["Direct bradyzoite-gene promoter occupancy not shown as the repression mechanism","Whether repression is direct or a consequence of cell-cycle arrest unresolved","In vivo relevance to chronic infection not established"],"attribution":[{"species":"Toxoplasma gondii","strain":"RH"},{"species":"Toxoplasma gondii","strain":"Pru"}]},{"year":null,"claim":"The DNA motif recognized by the AP2XII-9 AP2 domain, its chromatin/co-regulator partners, and whether bradyzoite-gene repression is direct remain unresolved.","evidence":"","pmids":[],"confidence":"High","gaps":["No defined consensus binding motif","No identified protein interaction partners or complexes","Direct versus indirect basis of bradyzoite repression unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["TGAP2III-2 (FUNCTIONAL/GENETIC INTERACTION IN DAUGHTER CELL ASSEMBLY; AP2XII-9 DOMINANT)"],"other_free_text":["Direct promoter targets include moving junction machinery genes RON2, RON4, RON8 and daughter inner membrane complex genes [#0].","Activates de novo microneme formation genes and represses budding-initiation genes across ~300 promoters [#2].","AP2 DNA-binding domain is required for function [#2].","Protein expression oscillates with the cell cycle, peaking in S/M phase [#0, #3].","Represses bradyzoite-specific genes independent of alkaline stress [#1]."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"20865045","id":"PMC_20865045","title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.","date":"2010","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/20865045","citation_count":234,"is_preprint":false},{"pmid":"29932347","id":"PMC_29932347","title":"The Epigenome, Cell Cycle, and Development in Toxoplasma.","date":"2018","source":"Annual review of microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/29932347","citation_count":51,"is_preprint":false},{"pmid":"20657661","id":"PMC_20657661","title":"The construction and use of log-odds substitution scores for multiple sequence alignment.","date":"2010","source":"PLoS computational biology","url":"https://pubmed.ncbi.nlm.nih.gov/20657661","citation_count":48,"is_preprint":false},{"pmid":"22486860","id":"PMC_22486860","title":"Cactin is essential for G1 progression in Toxoplasma gondii.","date":"2012","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/22486860","citation_count":23,"is_preprint":false},{"pmid":"25393307","id":"PMC_25393307","title":"Distinct strains of Toxoplasma gondii feature divergent transcriptomes regardless of developmental stage.","date":"2014","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/25393307","citation_count":22,"is_preprint":false},{"pmid":"39207100","id":"PMC_39207100","title":"Cell cycle-regulated transcription factor AP2XII-9 is a key activator for asexual division and apicoplast inheritance in Toxoplasma gondii tachyzoite.","date":"2024","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/39207100","citation_count":8,"is_preprint":false},{"pmid":"40106192","id":"PMC_40106192","title":"AP2XII-9 is essential for parasite growth and suppresses bradyzoite differentiation in Toxoplasma gondii.","date":"2025","source":"FASEB journal : official publication of the Federation of American Societies for Experimental Biology","url":"https://pubmed.ncbi.nlm.nih.gov/40106192","citation_count":3,"is_preprint":false},{"pmid":"39774584","id":"PMC_39774584","title":"Cascading expression of ApiAP2 transcription factors controls daughter cell assembly in Toxoplasma gondii.","date":"2024","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/39774584","citation_count":3,"is_preprint":false},{"pmid":"42087241","id":"PMC_42087241","title":"Host-cell dependent epigenetic profiles associated with survival outcomes in T. gondii infection.","date":"2026","source":"Epigenetics & chromatin","url":"https://pubmed.ncbi.nlm.nih.gov/42087241","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":14215,"output_tokens":1393,"usd":0.03177,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6509,"output_tokens":2824,"usd":0.051573,"stage2_stop_reason":"end_turn"},"total_usd":0.083343,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"paper list: removed papers about other genes"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2024,\n      \"finding\": \"TgAP2XII-9 is a nuclear transcription factor expressed predominantly during the S/M phase of the tachyzoite cell cycle. CUT&Tag experiments showed it directly targets promoters of moving junction machinery genes (RON2, RON4, RON8) and daughter cell inner membrane complex (IMC) genes. Conditional knockdown of TgAP2XII-9 caused significant downregulation of rhoptry proteins and rhoptry neck proteins, leading to severe defects in invasion and egress efficiency, as well as disorders in daughter bud formation and apicoplast inheritance, resulting in failure of cell division and intracellular proliferation.\",\n      \"method\": \"CUT&Tag, conditional knockdown, invasion/egress assays, transcriptomic analysis\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — conditional knockdown with defined cellular phenotypes plus CUT&Tag genome-wide binding data linking AP2XII-9 directly to target gene promoters, single lab but multiple orthogonal methods\",\n      \"pmids\": [\"39207100\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"AP2XII-9 localizes to the nucleus with periodic expression peaking during S/M phase. Conditional knockdown in both type I RH and type II Pru strains demonstrated that AP2XII-9 is essential for proper inner membrane complex formation, apicoplast inheritance, and normal cell division. Transcriptomic analysis showed that AP2XII-9 depletion caused downregulation of tachyzoite growth-related genes and upregulation of bradyzoite-specific genes, indicating AP2XII-9 actively represses bradyzoite differentiation even in the absence of alkaline stress.\",\n      \"method\": \"Conditional knockdown, transcriptomic analysis, immunofluorescence/localization\",\n      \"journal\": \"FASEB journal\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — conditional knockdown with defined phenotypic readouts (IMC, apicoplast, cell division, differentiation) replicated in two strains (RH and Pru) with transcriptomic support\",\n      \"pmids\": [\"40106192\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"RH\"},\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"Pru\"}\n      ]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"TgAP2XII-9 regulates daughter cell assembly by controlling transcription of at least 300 gene promoters. CUT&Tag and transcriptomics demonstrated that TgAP2XII-9 represses genes necessary for budding initiation while activating genes required for microneme de novo formation. The AP2 DNA-binding domain of TgAP2XII-9 was shown to be critical for its function via domain mutagenesis experiments. A double mutant of TgAP2XII-9 and TgAP2III-2 showed TgAP2XII-9 has the prominent role in daughter cell assembly.\",\n      \"method\": \"CUT&Tag, transcriptomics, AP2 domain mutagenesis, double-mutant epistasis\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — multiple orthogonal methods including CUT&Tag, transcriptomics, domain mutagenesis, and genetic epistasis in a single study\",\n      \"pmids\": [\"39774584\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2010,\n      \"finding\": \"AP2XII-9 (identified as one of two dozen AP2 transcription factors) shows a cyclical protein expression profile in the tachyzoite cell cycle, with a sharp peak of protein expression in the S/M-subtranscriptome timeframe, suggesting the timing of protein delivery may be mediated by AP2 domain promoter recognition specificities.\",\n      \"method\": \"Cell cycle transcriptome profiling, protein expression timing analysis\",\n      \"journal\": \"PloS one\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — expression profiling only, no direct functional characterization of AP2XII-9 specifically\",\n      \"pmids\": [\"20865045\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"AP2XII-9 (TgAP2XII-9) is a nuclear transcription factor expressed predominantly during the S/M phase of the tachyzoite cell cycle that acts as a key activator of genes required for daughter cell inner membrane complex assembly, moving junction machinery (RON2/4/8), rhoptry proteins, microneme formation, and apicoplast inheritance, while simultaneously repressing bradyzoite differentiation genes; conditional loss of AP2XII-9 causes severe defects in endodyogeny, apicoplast inheritance, invasion, and egress, and triggers aberrant bradyzoite differentiation, establishing AP2XII-9 as an essential S/M-phase regulator that coordinates tachyzoite replication and suppresses developmental stage conversion.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"AP2XII-9 (TgAP2XII-9) is a nuclear AP2-family transcription factor that operates as an S/M-phase master regulator of the Toxoplasma gondii tachyzoite cell cycle, coordinating daughter cell formation while suppressing developmental stage conversion [#0, #1]. Its protein abundance oscillates through the cell cycle, peaking sharply during the S/M-subtranscriptome window [#0, #3], consistent with a role in delivering a transcriptional program at the point of division. Through its AP2 DNA-binding domain, which is required for function, it directly occupies several hundred gene promoters, activating genes for moving junction machinery (RON2, RON4, RON8), the daughter inner membrane complex, and de novo microneme formation, while repressing genes governing budding initiation [#0, #2]. Conditional knockdown, replicated in type I RH and type II Pru strains, causes downregulation of rhoptry and rhoptry-neck proteins and of tachyzoite growth genes, producing defective daughter bud formation, disrupted apicoplast inheritance, impaired invasion and egress, and failure of intracellular proliferation [#0, #1]. Depletion also derepresses bradyzoite-specific genes even without alkaline stress, establishing AP2XII-9 as an active repressor of bradyzoite differentiation [#1]. Epistasis with TgAP2III-2 shows AP2XII-9 holds the dominant role in daughter cell assembly [#2].\",\n  \"teleology\": [\n    {\n      \"year\": 2010,\n      \"claim\": \"Establishing whether AP2XII-9 is cell-cycle regulated was the first step in linking it to a specific replicative window; profiling showed its protein peaks sharply in the S/M phase, implicating it in division-associated transcription.\",\n      \"evidence\": \"Cell cycle transcriptome and protein expression timing profiling in tachyzoites\",\n      \"pmids\": [\"20865045\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Expression profiling only; no functional characterization of AP2XII-9 specifically\",\n        \"No promoter targets or DNA-binding activity demonstrated\",\n        \"No phenotype from perturbation\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"To determine what AP2XII-9 does mechanistically, genome-wide binding and conditional depletion were combined, showing it is a nuclear S/M-phase factor that directly targets moving junction and IMC promoters and is required for invasion, egress, budding, and apicoplast inheritance.\",\n      \"evidence\": \"CUT&Tag, conditional knockdown, invasion/egress assays, and transcriptomics\",\n      \"pmids\": [\"39207100\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Single lab and strain background\",\n        \"Direct versus indirect effects on rhoptry protein levels not fully separated\",\n        \"Co-regulators or chromatin partners not identified\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Whether the AP2 domain drives function and how AP2XII-9 ranks among related factors was resolved by domain mutagenesis and epistasis, showing the AP2 DNA-binding domain is critical and that AP2XII-9 dominates daughter cell assembly over TgAP2III-2 by both activating microneme genes and repressing budding-initiation genes across ~300 promoters.\",\n      \"evidence\": \"CUT&Tag, transcriptomics, AP2 domain mutagenesis, and double-mutant epistasis\",\n      \"pmids\": [\"39774584\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"DNA sequence motif recognized by the AP2 domain not defined\",\n        \"Mechanism distinguishing activated versus repressed targets unresolved\",\n        \"Nature of the AP2XII-9/AP2III-2 functional relationship not mechanistically detailed\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether AP2XII-9's role extends to controlling developmental fate was addressed by conditional knockdown in two strains, showing depletion downregulates tachyzoite growth genes and upregulates bradyzoite-specific genes even without alkaline stress, establishing AP2XII-9 as an active repressor of stage conversion.\",\n      \"evidence\": \"Conditional knockdown in RH and Pru strains, transcriptomics, and immunofluorescence localization\",\n      \"pmids\": [\"40106192\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"Direct bradyzoite-gene promoter occupancy not shown as the repression mechanism\",\n        \"Whether repression is direct or a consequence of cell-cycle arrest unresolved\",\n        \"In vivo relevance to chronic infection not established\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"RH\"},\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"Pru\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The DNA motif recognized by the AP2XII-9 AP2 domain, its chromatin/co-regulator partners, and whether bradyzoite-gene repression is direct remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"No defined consensus binding motif\",\n        \"No identified protein interaction partners or complexes\",\n        \"Direct versus indirect basis of bradyzoite repression unknown\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0003700\", \"supporting_discovery_ids\": [0, 1, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0003677\", \"supporting_discovery_ids\": [2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0000981\", \"supporting_discovery_ids\": [0, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0051301\", \"supporting_discovery_ids\": [0, 1, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0006355\", \"supporting_discovery_ids\": [0, 1, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0030154\", \"supporting_discovery_ids\": [1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [],\n    \"partners\": [\n      \"TgAP2III-2 (functional/genetic interaction in daughter cell assembly; AP2XII-9 dominant)\"\n    ],\n    \"other_free_text\": [\n      \"Direct promoter targets include moving junction machinery genes RON2, RON4, RON8 and daughter inner membrane complex genes [#0].\",\n      \"Activates de novo microneme formation genes and represses budding-initiation genes across ~300 promoters [#2].\",\n      \"AP2 DNA-binding domain is required for function [#2].\",\n      \"Protein expression oscillates with the cell cycle, peaking in S/M phase [#0, #3].\",\n      \"Represses bradyzoite-specific genes independent of alkaline stress [#1].\"\n    ]\n  },\n  \"strain_conflicts\": []\n}\n```","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_251740","name":"AP2XII9","product":"AP2 domain transcription factor AP2XII-9","uniprot_acc":"A0A125YNT3","chromosome":"TGME49_chrXII","start_pos":5551922},{"strain":"GT1","gene_id":"TGGT1_251740","name":null,"product":"AP2 domain transcription factor AP2XII-9","uniprot_acc":null,"chromosome":"TGGT1_chrXII","start_pos":5473941},{"strain":"RH88","gene_id":"TGRH88_064590","name":null,"product":"AP2 domain transcription factor AP2XII-9","uniprot_acc":null,"chromosome":"CM023093","start_pos":5633160},{"strain":"VEG","gene_id":"TGVEG_251740","name":null,"product":"AP2 domain transcription factor AP2XII-9","uniprot_acc":"B6KHU0","chromosome":"TGVEG_chrXII","start_pos":5546340}]}