{"gene":"TG_238895","gene_symbol":"IMC43","run_date":"2026-09-08T15:31:33+00:00","timeline":{"discoveries":[{"year":2023,"finding":"IMC43 is a novel daughter IMC protein recruited at the earliest stages of daughter bud initiation during endodyogeny in Toxoplasma gondii.","method":"Fluorescence microscopy / localization studies during endodyogeny","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Moderate — direct localization experiments with functional consequence, single lab with multiple orthogonal methods","pmids":["37782662"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"finding":"Depletion of IMC43 via auxin-inducible degron results in aberrant parasite morphology, dysregulation of endodyogeny, and severe replication defects, demonstrating that IMC43 is essential for parasite fitness.","method":"Auxin-inducible degron (AID) system / conditional depletion with phenotypic readout","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Moderate — clean conditional KO with specific cellular phenotypes, single lab with multiple readouts","pmids":["37782662"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"finding":"A C-terminal domain of IMC43 is essential for the protein's function, and a region within IMC43 plays a role in its localization, as determined by deletion analyses.","method":"Deletion analysis / domain mapping with functional complementation","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — deletion mutagenesis with localization and functional readouts, single lab","pmids":["37782662"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"finding":"IMC43 directly interacts with IMC32 and IMC44, as identified by TurboID proximity labeling and yeast two-hybrid screens, and the C-terminal domain of IMC43 is sufficient for binding to both IMC32 and IMC44.","method":"TurboID proximity labeling, yeast two-hybrid assay (IMC43 as bait), pairwise yeast two-hybrid assays","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Moderate — reciprocal yeast two-hybrid plus proximity labeling, two orthogonal methods in single lab","pmids":["37782662"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"finding":"IMC43 regulates the localization of both IMC32 and IMC44 at specific stages of endodyogeny, and this regulation depends on the essential C-terminal domain of IMC43.","method":"Conditional depletion (AID) of IMC43 followed by fluorescence microscopy of IMC32 and IMC44 localization","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Moderate — genetic depletion with specific localization readout for two partner proteins, single lab with multiple orthogonal approaches","pmids":["37782662"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"finding":"IMC43 and IMC32 together form an essential daughter bud assembly complex that lays the foundation of the daughter IMC scaffold during endodyogeny.","method":"Genetic depletion, proximity labeling, yeast two-hybrid, and localization analyses","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Strong — multiple orthogonal methods establishing complex membership and essentiality, replicated in follow-up study","pmids":["37782662","39024411"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"finding":"BCC0 localization to daughter buds depends on the presence of both IMC32 and IMC43, establishing IMC43 as required for BCC0 recruitment to the nascent bud scaffold.","method":"Conditional depletion of IMC32 and IMC43 followed by fluorescence microscopy of BCC0 localization","journal":"PLoS pathogens","confidence":"High","confidence_rationale":"Tier 2 / Moderate — genetic depletion of two independent proteins with specific localization readout, single lab","pmids":["39024411"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"finding":"BCC0 and IMC43 do not directly interact with each other; instead, BCC0 binds to the coiled-coil region of IMC32, and an IMC32-BCC0 subcomplex initially recruits to nascent buds via palmitoylation of IMC32 before IMC32 binds to IMC43 to lock into the scaffold during bud elongation.","method":"Pairwise yeast two-hybrid assays, proximity labeling, deletion and complementation analyses","journal":"PLoS pathogens","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — pairwise yeast two-hybrid with multiple deletion analyses, negative BCC0–IMC43 interaction is itself mechanistically informative, single lab","pmids":["39024411"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC43 is an essential early-recruiting daughter IMC protein in Toxoplasma gondii that, together with IMC32 and BCC0, forms a tripartite bud assembly complex at the earliest stages of endodyogeny; its C-terminal domain directly binds IMC32 and IMC44 and is required to regulate their localization and to scaffold the nascent daughter IMC, with IMC32–BCC0 first recruited via palmitoylation and then locked into the scaffold by IMC32 binding to IMC43 during bud elongation."},"narrative":{"mechanistic_narrative":"IMC43 is an essential daughter inner membrane complex (IMC) protein in Toxoplasma gondii that is recruited at the earliest stages of daughter bud initiation and scaffolds nascent daughter IMC assembly during endodyogeny [PMID:37782662]. Conditional depletion produces aberrant morphology, dysregulated endodyogeny, and severe replication defects, establishing IMC43 as required for parasite fitness [PMID:37782662]. IMC43 acts through its essential C-terminal domain, which directly binds IMC32 and IMC44 and is required to regulate the stage-specific localization of both partners [PMID:37782662]. Together IMC43 and IMC32 form an essential daughter bud assembly complex that founds the daughter IMC scaffold [PMID:37782662, PMID:39024411], and IMC43 is additionally required for recruitment of BCC0 to nascent buds [PMID:39024411]. BCC0 does not bind IMC43 directly but associates with the coiled-coil region of IMC32; an IMC32–BCC0 subcomplex is first recruited to buds via palmitoylation of IMC32 and is subsequently locked into the scaffold when IMC32 engages IMC43 during bud elongation [PMID:39024411].","teleology":[{"year":2023,"claim":"Identifying when and where a new IMC component appears during replication established IMC43 as an early marker of daughter bud formation, defining the temporal window it acts in.","evidence":"fluorescence microscopy localization across endodyogeny in Toxoplasma gondii","pmids":["37782662"],"confidence":"High","gaps":["localization alone does not establish function","recruitment signal directing IMC43 to nascent buds not defined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"claim":"Conditional depletion tested whether IMC43 is dispensable and showed it is essential, linking loss of the protein directly to failed daughter cell assembly and replication.","evidence":"auxin-inducible degron depletion with morphological and replication readouts","pmids":["37782662"],"confidence":"High","gaps":["does not resolve which molecular interactions underlie the phenotype","timing of the block within endodyogeny not fully dissected"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"claim":"Domain mapping determined which part of IMC43 carries its function and localization determinants, pinpointing the C-terminal domain as the essential functional module.","evidence":"deletion analysis with localization and functional complementation","pmids":["37782662"],"confidence":"Medium","gaps":["no structural model of the C-terminal domain","residue-level binding determinants not defined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"claim":"Interaction mapping asked what IMC43 binds and showed its C-terminal domain directly engages IMC32 and IMC44, providing the molecular basis for its scaffolding role.","evidence":"TurboID proximity labeling and pairwise yeast two-hybrid with IMC43 as bait","pmids":["37782662"],"confidence":"High","gaps":["binding affinities and stoichiometry unknown","no reconstitution of the complex in vitro"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2023,"claim":"Depleting IMC43 and tracking its partners showed it governs the stage-specific localization of IMC32 and IMC44, elevating IMC43 from a binding partner to an organizing scaffold for the daughter IMC.","evidence":"AID depletion of IMC43 followed by fluorescence microscopy of IMC32 and IMC44","pmids":["37782662"],"confidence":"High","gaps":["mechanism by which IMC43 positions partners not resolved","does not define the assembly order relative to other IMC components"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"claim":"Extending the depletion approach to BCC0 established IMC43 (with IMC32) as required for BCC0 recruitment, expanding the assembly complex beyond the IMC43–IMC32 core.","evidence":"conditional depletion of IMC32 and IMC43 with fluorescence microscopy of BCC0","pmids":["39024411"],"confidence":"High","gaps":["does not distinguish direct versus indirect recruitment routes on its own"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2024,"claim":"Dissecting the wiring of the tripartite complex resolved the recruitment order, showing BCC0 binds IMC32 (not IMC43) and that a palmitoylation-primed IMC32–BCC0 subcomplex is subsequently locked into the scaffold via IMC32–IMC43 binding during bud elongation.","evidence":"pairwise yeast two-hybrid, proximity labeling, and deletion/complementation analyses","pmids":["39024411"],"confidence":"Medium","gaps":["palmitoylation enzyme(s) for IMC32 not identified","temporal transition from initial recruitment to lock-in not directly imaged","single lab, no in vitro reconstitution"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The structural basis of IMC43 scaffolding and how its assembly is regulated in space and time during bud elongation remain open.","evidence":"no structural or in vitro reconstitution data in the available corpus","pmids":[],"confidence":"Medium","gaps":["no atomic structure of IMC43 or its complexes","functional role of IMC44 beyond binding not established","regulatory signals timing the IMC32–IMC43 lock-in unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["IMC43–IMC32 daughter bud assembly complex","IMC43–IMC32–BCC0 tripartite bud scaffold complex"],"partners":["IMC32 (DIRECT, VIA IMC43 C-TERMINAL DOMAIN)","IMC44 (DIRECT, VIA IMC43 C-TERMINAL DOMAIN)","BCC0 (INDIRECT; BINDS IMC32 COILED-COIL, NOT IMC43)"],"other_free_text":["IMC43 is recruited at the earliest stage of daughter bud initiation during endodyogeny.","IMC43 depletion causes aberrant morphology, dysregulated endodyogeny, and severe replication defects.","The essential C-terminal domain mediates both partner binding and regulation of IMC32/IMC44 localization.","IMC32–BCC0 subcomplex is recruited to buds via palmitoylation of IMC32, then locked into the scaffold by IMC32–IMC43 binding during bud elongation."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"37782662","id":"PMC_37782662","title":"Identification of IMC43, a novel IMC protein that collaborates with IMC32 to form an essential daughter bud assembly complex in Toxoplasma gondii.","date":"2023","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/37782662","citation_count":11,"is_preprint":false},{"pmid":"39024411","id":"PMC_39024411","title":"BCC0 collaborates with IMC32 and IMC43 to form the Toxoplasma gondii essential daughter bud assembly complex.","date":"2024","source":"PLoS pathogens","url":"https://pubmed.ncbi.nlm.nih.gov/39024411","citation_count":7,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3437,"output_tokens":1822,"usd":0.018821,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":7096,"output_tokens":2972,"usd":0.05489,"stage2_stop_reason":"end_turn"},"total_usd":0.0,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC43 is a novel daughter IMC protein recruited at the earliest stages of daughter bud initiation during endodyogeny in Toxoplasma gondii.\",\n      \"method\": \"Fluorescence microscopy / localization studies during endodyogeny\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization experiments with functional consequence, single lab with multiple orthogonal methods\",\n      \"pmids\": [\"37782662\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"Depletion of IMC43 via auxin-inducible degron results in aberrant parasite morphology, dysregulation of endodyogeny, and severe replication defects, demonstrating that IMC43 is essential for parasite fitness.\",\n      \"method\": \"Auxin-inducible degron (AID) system / conditional depletion with phenotypic readout\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean conditional KO with specific cellular phenotypes, single lab with multiple readouts\",\n      \"pmids\": [\"37782662\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"A C-terminal domain of IMC43 is essential for the protein's function, and a region within IMC43 plays a role in its localization, as determined by deletion analyses.\",\n      \"method\": \"Deletion analysis / domain mapping with functional complementation\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — deletion mutagenesis with localization and functional readouts, single lab\",\n      \"pmids\": [\"37782662\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC43 directly interacts with IMC32 and IMC44, as identified by TurboID proximity labeling and yeast two-hybrid screens, and the C-terminal domain of IMC43 is sufficient for binding to both IMC32 and IMC44.\",\n      \"method\": \"TurboID proximity labeling, yeast two-hybrid assay (IMC43 as bait), pairwise yeast two-hybrid assays\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal yeast two-hybrid plus proximity labeling, two orthogonal methods in single lab\",\n      \"pmids\": [\"37782662\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC43 regulates the localization of both IMC32 and IMC44 at specific stages of endodyogeny, and this regulation depends on the essential C-terminal domain of IMC43.\",\n      \"method\": \"Conditional depletion (AID) of IMC43 followed by fluorescence microscopy of IMC32 and IMC44 localization\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — genetic depletion with specific localization readout for two partner proteins, single lab with multiple orthogonal approaches\",\n      \"pmids\": [\"37782662\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"finding\": \"IMC43 and IMC32 together form an essential daughter bud assembly complex that lays the foundation of the daughter IMC scaffold during endodyogeny.\",\n      \"method\": \"Genetic depletion, proximity labeling, yeast two-hybrid, and localization analyses\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Strong — multiple orthogonal methods establishing complex membership and essentiality, replicated in follow-up study\",\n      \"pmids\": [\"37782662\", \"39024411\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"BCC0 localization to daughter buds depends on the presence of both IMC32 and IMC43, establishing IMC43 as required for BCC0 recruitment to the nascent bud scaffold.\",\n      \"method\": \"Conditional depletion of IMC32 and IMC43 followed by fluorescence microscopy of BCC0 localization\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — genetic depletion of two independent proteins with specific localization readout, single lab\",\n      \"pmids\": [\"39024411\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"finding\": \"BCC0 and IMC43 do not directly interact with each other; instead, BCC0 binds to the coiled-coil region of IMC32, and an IMC32-BCC0 subcomplex initially recruits to nascent buds via palmitoylation of IMC32 before IMC32 binds to IMC43 to lock into the scaffold during bud elongation.\",\n      \"method\": \"Pairwise yeast two-hybrid assays, proximity labeling, deletion and complementation analyses\",\n      \"journal\": \"PLoS pathogens\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — pairwise yeast two-hybrid with multiple deletion analyses, negative BCC0–IMC43 interaction is itself mechanistically informative, single lab\",\n      \"pmids\": [\"39024411\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"IMC43 is an essential early-recruiting daughter IMC protein in Toxoplasma gondii that, together with IMC32 and BCC0, forms a tripartite bud assembly complex at the earliest stages of endodyogeny; its C-terminal domain directly binds IMC32 and IMC44 and is required to regulate their localization and to scaffold the nascent daughter IMC, with IMC32–BCC0 first recruited via palmitoylation and then locked into the scaffold by IMC32 binding to IMC43 during bud elongation.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC43 is an essential daughter inner membrane complex (IMC) protein in Toxoplasma gondii that is recruited at the earliest stages of daughter bud initiation and scaffolds nascent daughter IMC assembly during endodyogeny [#0]. Conditional depletion produces aberrant morphology, dysregulated endodyogeny, and severe replication defects, establishing IMC43 as required for parasite fitness [#1]. IMC43 acts through its essential C-terminal domain, which directly binds IMC32 and IMC44 and is required to regulate the stage-specific localization of both partners [#2, #3, #4]. Together IMC43 and IMC32 form an essential daughter bud assembly complex that founds the daughter IMC scaffold [#5], and IMC43 is additionally required for recruitment of BCC0 to nascent buds [#6]. BCC0 does not bind IMC43 directly but associates with the coiled-coil region of IMC32; an IMC32\\u2013BCC0 subcomplex is first recruited to buds via palmitoylation of IMC32 and is subsequently locked into the scaffold when IMC32 engages IMC43 during bud elongation [#7].\",\n  \"teleology\": [\n    {\n      \"year\": 2023,\n      \"claim\": \"Identifying when and where a new IMC component appears during replication established IMC43 as an early marker of daughter bud formation, defining the temporal window it acts in.\",\n      \"evidence\": \"fluorescence microscopy localization across endodyogeny in Toxoplasma gondii\",\n      \"pmids\": [\"37782662\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"localization alone does not establish function\", \"recruitment signal directing IMC43 to nascent buds not defined\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Conditional depletion tested whether IMC43 is dispensable and showed it is essential, linking loss of the protein directly to failed daughter cell assembly and replication.\",\n      \"evidence\": \"auxin-inducible degron depletion with morphological and replication readouts\",\n      \"pmids\": [\"37782662\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"does not resolve which molecular interactions underlie the phenotype\", \"timing of the block within endodyogeny not fully dissected\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Domain mapping determined which part of IMC43 carries its function and localization determinants, pinpointing the C-terminal domain as the essential functional module.\",\n      \"evidence\": \"deletion analysis with localization and functional complementation\",\n      \"pmids\": [\"37782662\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"no structural model of the C-terminal domain\", \"residue-level binding determinants not defined\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Interaction mapping asked what IMC43 binds and showed its C-terminal domain directly engages IMC32 and IMC44, providing the molecular basis for its scaffolding role.\",\n      \"evidence\": \"TurboID proximity labeling and pairwise yeast two-hybrid with IMC43 as bait\",\n      \"pmids\": [\"37782662\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"binding affinities and stoichiometry unknown\", \"no reconstitution of the complex in vitro\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2023,\n      \"claim\": \"Depleting IMC43 and tracking its partners showed it governs the stage-specific localization of IMC32 and IMC44, elevating IMC43 from a binding partner to an organizing scaffold for the daughter IMC.\",\n      \"evidence\": \"AID depletion of IMC43 followed by fluorescence microscopy of IMC32 and IMC44\",\n      \"pmids\": [\"37782662\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"mechanism by which IMC43 positions partners not resolved\", \"does not define the assembly order relative to other IMC components\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Extending the depletion approach to BCC0 established IMC43 (with IMC32) as required for BCC0 recruitment, expanding the assembly complex beyond the IMC43\\u2013IMC32 core.\",\n      \"evidence\": \"conditional depletion of IMC32 and IMC43 with fluorescence microscopy of BCC0\",\n      \"pmids\": [\"39024411\"],\n      \"confidence\": \"High\",\n      \"gaps\": [\"does not distinguish direct versus indirect recruitment routes on its own\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2024,\n      \"claim\": \"Dissecting the wiring of the tripartite complex resolved the recruitment order, showing BCC0 binds IMC32 (not IMC43) and that a palmitoylation-primed IMC32\\u2013BCC0 subcomplex is subsequently locked into the scaffold via IMC32\\u2013IMC43 binding during bud elongation.\",\n      \"evidence\": \"pairwise yeast two-hybrid, proximity labeling, and deletion/complementation analyses\",\n      \"pmids\": [\"39024411\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"palmitoylation enzyme(s) for IMC32 not identified\", \"temporal transition from initial recruitment to lock-in not directly imaged\", \"single lab, no in vitro reconstitution\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The structural basis of IMC43 scaffolding and how its assembly is regulated in space and time during bud elongation remain open.\",\n      \"evidence\": \"no structural or in vitro reconstitution data in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\"no atomic structure of IMC43 or its complexes\", \"functional role of IMC44 beyond binding not established\", \"regulatory signals timing the IMC32\\u2013IMC43 lock-in unknown\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0005198\", \"supporting_discovery_ids\": [0, 5], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0005515\", \"supporting_discovery_ids\": [3, 7], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0070258\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0032506\", \"supporting_discovery_ids\": [1, 5], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [\n      \"IMC43\\u2013IMC32 daughter bud assembly complex\",\n      \"IMC43\\u2013IMC32\\u2013BCC0 tripartite bud scaffold complex\"\n    ],\n    \"partners\": [\n      \"IMC32 (direct, via IMC43 C-terminal domain)\",\n      \"IMC44 (direct, via IMC43 C-terminal domain)\",\n      \"BCC0 (indirect; binds IMC32 coiled-coil, not IMC43)\"\n    ],\n    \"other_free_text\": [\n      \"IMC43 is recruited at the earliest stage of daughter bud initiation during endodyogeny.\",\n      \"IMC43 depletion causes aberrant morphology, dysregulated endodyogeny, and severe replication defects.\",\n      \"The essential C-terminal domain mediates both partner binding and regulation of IMC32/IMC44 localization.\",\n      \"IMC32\\u2013BCC0 subcomplex is recruited to buds via palmitoylation of IMC32, then locked into the scaffold by IMC32\\u2013IMC43 binding during bud elongation.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_238895","name":"IMC43","product":"inner membrane complex protein IMC43","uniprot_acc":"S8GG99","chromosome":"TGME49_chrVI","start_pos":298259},{"strain":"GT1","gene_id":"TGGT1_238895","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVI","start_pos":292533},{"strain":"RH88","gene_id":"TGRH88_031890","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023087","start_pos":3397259},{"strain":"VEG","gene_id":"TGVEG_238895","name":null,"product":"hypothetical protein","uniprot_acc":"A0A125YLD8","chromosome":"TGVEG_chrVI","start_pos":311794}]}