{"gene":"TG_237090","gene_symbol":"AP2X5","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2018,"finding":"TgAP2X-5 was identified as a protein interacting with TgAP2XI-5 (a cell cycle-regulated ApiAP2 transcription factor active during S/M phase), establishing that AP2X-5 functions as part of a cooperative transcription factor complex.","method":"Co-immunoprecipitation / protein interaction screen identifying AP2X-5 as a binding partner of AP2XI-5","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — single Co-IP identifying the interaction, supported by functional follow-up in the same study","pmids":["29788176"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"finding":"Inducible knockdown of TgAP2X-5 using a conditional knock-down strategy combined with RNA-seq demonstrated that AP2X-5 is required for normal expression levels of multiple virulence factor transcripts, including rhoptry and microneme genes.","method":"Inducible knockdown (conditional knock-down) combined with RNA-seq transcriptomic analysis","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — conditional KD with defined transcriptomic phenotype, single lab, two orthogonal methods (KD + RNA-seq)","pmids":["29788176"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"finding":"Disruption of TgAP2X-5 expression led to defects in TgAP2XI-5 binding to multiple rhoptry gene promoters in a genome-wide chromatin assay, demonstrating that AP2X-5 is required for the cooperative promoter occupancy of AP2XI-5 at virulence gene loci.","method":"Chromatin immunoprecipitation genome-wide binding assay (ChIP) of AP2XI-5 in the AP2X-5 knockdown strain","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — genome-wide ChIP in a defined mutant background, single lab, mechanistically informative","pmids":["29788176"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"finding":"While disruption of TgAP2X-5 had only a mild effect on parasite invasion of host cells in vitro, it resulted in complete avirulence in a mouse infection model, establishing an in vivo requirement for AP2X-5 in parasite virulence.","method":"Mouse infection virulence assay with AP2X-5 conditional knockdown parasites; in vitro invasion assay","journal":"Nucleic acids research","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — in vivo mouse model and in vitro invasion assay, single lab","pmids":["29788176"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"finding":"TgAP2X-5 is a cell cycle-regulated ApiAP2 transcription factor whose expression is coordinated with the S/M phase of the tachyzoite cell cycle, consistent with its role in regulating S/M-phase virulence genes.","method":"Expression profiling / cell cycle staging of AP2X-5 protein and mRNA","journal":"Nucleic acids research","confidence":"Low","confidence_rationale":"Tier 3 / Weak — expression timing correlation from a single study without functional dissection of the cell cycle link","pmids":["29788176"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TgAP2X-5 is a cell cycle-regulated ApiAP2 transcription factor that cooperates with TgAP2XI-5 at the promoters of S/M-phase virulence genes (rhoptry and microneme genes); AP2X-5 is required for proper promoter occupancy by AP2XI-5, and its loss reduces virulence gene expression and renders parasites avirulent in mice despite only mild effects on in vitro invasion."},"narrative":{"mechanistic_narrative":"TgAP2X-5 is a cell cycle-regulated ApiAP2 transcription factor in Toxoplasma gondii that acts within a cooperative transcriptional complex to drive expression of S/M-phase virulence genes [PMID:29788176]. It was identified as a physical interaction partner of TgAP2XI-5 and functions to enable that factor's promoter occupancy: loss of AP2X-5 impairs AP2XI-5 binding across multiple rhoptry gene promoters genome-wide [PMID:29788176]. Consistent with this regulatory role, inducible knockdown of AP2X-5 reduces transcript levels of numerous virulence factors, including rhoptry and microneme genes [PMID:29788176]. This transcriptional deficit has a sharply compartmentalized functional consequence: AP2X-5 disruption only mildly affects host-cell invasion in vitro but renders parasites completely avirulent in mice, establishing it as an in vivo determinant of virulence [PMID:29788176].","teleology":[{"year":2018,"claim":"Establishing that AP2X-5 does not act alone but partners with another ApiAP2 factor defined it as a component of a cooperative transcription factor complex rather than an independent regulator.","evidence":"Co-immunoprecipitation / interaction screen identifying AP2X-5 as a binding partner of AP2XI-5 in T. gondii","pmids":["29788176"],"confidence":"Medium","gaps":["Single Co-IP without reciprocal validation or stoichiometry of the complex","Whether the interaction is direct or bridged by other factors is unresolved","DNA-binding specificity of AP2X-5 itself not defined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"claim":"Conditional knockdown coupled to transcriptomics answered whether AP2X-5 is functionally required for gene expression, showing it controls a defined virulence-gene program.","evidence":"Inducible conditional knockdown plus RNA-seq in T. gondii","pmids":["29788176"],"confidence":"Medium","gaps":["Does not distinguish direct AP2X-5 targets from downstream transcriptional effects","Full set of dysregulated loci and their promoter architecture not detailed here"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"claim":"Genome-wide ChIP of AP2XI-5 in an AP2X-5-depleted background answered the mechanistic question of how AP2X-5 acts, revealing it is required for the partner factor's promoter occupancy at virulence loci.","evidence":"Genome-wide ChIP of AP2XI-5 in the AP2X-5 knockdown strain","pmids":["29788176"],"confidence":"Medium","gaps":["Whether AP2X-5 itself binds these promoters directly not shown","Mechanism by which AP2X-5 stabilizes AP2XI-5 occupancy is unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"claim":"Comparing in vitro invasion with in vivo mouse infection resolved the biological consequence of AP2X-5 loss, showing its requirement is specific to virulence rather than basic invasion.","evidence":"Mouse infection virulence assay and in vitro invasion assay with AP2X-5 conditional knockdown parasites","pmids":["29788176"],"confidence":"Medium","gaps":["Which specific dysregulated virulence gene(s) account for the avirulence not pinpointed","Effect across parasite strains/lineages not tested"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2018,"claim":"Expression profiling linked AP2X-5 to the S/M phase of the tachyzoite cell cycle, positioning its activity to coincide with the timing of the virulence genes it regulates.","evidence":"Expression/cell cycle staging of AP2X-5 protein and mRNA","pmids":["29788176"],"confidence":"Low","gaps":["Correlative timing only, without functional dissection of the cell cycle dependence","Upstream regulators controlling AP2X-5 periodic expression unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The intrinsic DNA-binding specificity of AP2X-5, the direct versus indirect nature of its target repertoire, and the structural basis of its cooperation with AP2XI-5 remain undefined.","evidence":"","pmids":[],"confidence":"Low","gaps":["No DNA-binding motif or direct genome-wide occupancy map for AP2X-5 itself","No structural model of the AP2X-5/AP2XI-5 complex","Requirement across parasite strains not established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["AP2X-5 / AP2XI-5 ApiAP2 transcription factor complex"],"partners":["TGAP2XI-5"],"other_free_text":["Cell cycle-regulated, with expression coordinated to the S/M phase of the tachyzoite cell cycle","Required for expression of rhoptry and microneme (virulence factor) genes","Required for AP2XI-5 promoter occupancy at rhoptry gene loci","Dispensable for efficient in vitro host-cell invasion but essential for virulence in a mouse infection model"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"20865045","id":"PMC_20865045","title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.","date":"2010","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/20865045","citation_count":234,"is_preprint":false},{"pmid":"29932347","id":"PMC_29932347","title":"The Epigenome, Cell Cycle, and Development in Toxoplasma.","date":"2018","source":"Annual review of microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/29932347","citation_count":51,"is_preprint":false},{"pmid":"20657661","id":"PMC_20657661","title":"The construction and use of log-odds substitution scores for multiple sequence alignment.","date":"2010","source":"PLoS computational biology","url":"https://pubmed.ncbi.nlm.nih.gov/20657661","citation_count":48,"is_preprint":false},{"pmid":"29788176","id":"PMC_29788176","title":"Cooperative binding of ApiAP2 transcription factors is crucial for the expression of virulence genes in Toxoplasma gondii.","date":"2018","source":"Nucleic acids research","url":"https://pubmed.ncbi.nlm.nih.gov/29788176","citation_count":35,"is_preprint":false},{"pmid":"22486860","id":"PMC_22486860","title":"Cactin is essential for G1 progression in Toxoplasma gondii.","date":"2012","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/22486860","citation_count":23,"is_preprint":false},{"pmid":"25393307","id":"PMC_25393307","title":"Distinct strains of Toxoplasma gondii feature divergent transcriptomes regardless of developmental stage.","date":"2014","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/25393307","citation_count":22,"is_preprint":false},{"pmid":"18983845","id":"PMC_18983845","title":"New eukaryotic systematics: a phylogenetic perspective of developmental gene expression in the Apicomplexa.","date":"2008","source":"International journal for parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/18983845","citation_count":12,"is_preprint":false},{"pmid":"39690155","id":"PMC_39690155","title":"Apicomplexan mitoribosome from highly fragmented rRNAs to a functional machine.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/39690155","citation_count":9,"is_preprint":false},{"pmid":"36178892","id":"PMC_36178892","title":"Genomic insights into host and parasite interactions during intracellular infection by Toxoplasma gondii.","date":"2022","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/36178892","citation_count":2,"is_preprint":false},{"pmid":"42087241","id":"PMC_42087241","title":"Host-cell dependent epigenetic profiles associated with survival outcomes in T. gondii infection.","date":"2026","source":"Epigenetics & chromatin","url":"https://pubmed.ncbi.nlm.nih.gov/42087241","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":18746,"output_tokens":1437,"usd":0.038897,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6264,"output_tokens":2418,"usd":0.045885,"stage2_stop_reason":"end_turn"},"total_usd":0.084782,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"paper list: removed papers about other genes"},"stage1_raw":"Looking through the abstracts, I need to find papers specifically about AP2X5 (also written as TgAP2X-5 or AP2X-5).\n\nPaper [9] (PMID:29788176) is clearly about TgAP2X-5 specifically - it describes TgAP2XI-5 interacting with TgAP2X-5, and characterizes TgAP2X-5's role experimentally.\n\nLet me also check paper [1] (PMID:20865045) - mentions AP2 factors in cell cycle but doesn't specifically characterize AP2X5 mechanistically.\n\nPaper [9] is the key paper about AP2X-5 (TgAP2X-5). Let me extract the mechanistic findings from it carefully.\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2018,\n      \"finding\": \"TgAP2X-5 was identified as a protein interacting with TgAP2XI-5 (a cell cycle-regulated ApiAP2 transcription factor active during S/M phase), establishing that AP2X-5 functions as part of a cooperative transcription factor complex.\",\n      \"method\": \"Co-immunoprecipitation / protein interaction screen identifying AP2X-5 as a binding partner of AP2XI-5\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — single Co-IP identifying the interaction, supported by functional follow-up in the same study\",\n      \"pmids\": [\"29788176\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"Inducible knockdown of TgAP2X-5 using a conditional knock-down strategy combined with RNA-seq demonstrated that AP2X-5 is required for normal expression levels of multiple virulence factor transcripts, including rhoptry and microneme genes.\",\n      \"method\": \"Inducible knockdown (conditional knock-down) combined with RNA-seq transcriptomic analysis\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — conditional KD with defined transcriptomic phenotype, single lab, two orthogonal methods (KD + RNA-seq)\",\n      \"pmids\": [\"29788176\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"Disruption of TgAP2X-5 expression led to defects in TgAP2XI-5 binding to multiple rhoptry gene promoters in a genome-wide chromatin assay, demonstrating that AP2X-5 is required for the cooperative promoter occupancy of AP2XI-5 at virulence gene loci.\",\n      \"method\": \"Chromatin immunoprecipitation genome-wide binding assay (ChIP) of AP2XI-5 in the AP2X-5 knockdown strain\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — genome-wide ChIP in a defined mutant background, single lab, mechanistically informative\",\n      \"pmids\": [\"29788176\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"While disruption of TgAP2X-5 had only a mild effect on parasite invasion of host cells in vitro, it resulted in complete avirulence in a mouse infection model, establishing an in vivo requirement for AP2X-5 in parasite virulence.\",\n      \"method\": \"Mouse infection virulence assay with AP2X-5 conditional knockdown parasites; in vitro invasion assay\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — in vivo mouse model and in vitro invasion assay, single lab\",\n      \"pmids\": [\"29788176\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"finding\": \"TgAP2X-5 is a cell cycle-regulated ApiAP2 transcription factor whose expression is coordinated with the S/M phase of the tachyzoite cell cycle, consistent with its role in regulating S/M-phase virulence genes.\",\n      \"method\": \"Expression profiling / cell cycle staging of AP2X-5 protein and mRNA\",\n      \"journal\": \"Nucleic acids research\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — expression timing correlation from a single study without functional dissection of the cell cycle link\",\n      \"pmids\": [\"29788176\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"TgAP2X-5 is a cell cycle-regulated ApiAP2 transcription factor that cooperates with TgAP2XI-5 at the promoters of S/M-phase virulence genes (rhoptry and microneme genes); AP2X-5 is required for proper promoter occupancy by AP2XI-5, and its loss reduces virulence gene expression and renders parasites avirulent in mice despite only mild effects on in vitro invasion.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"TgAP2X-5 is a cell cycle-regulated ApiAP2 transcription factor in Toxoplasma gondii that acts within a cooperative transcriptional complex to drive expression of S/M-phase virulence genes [#0, #1]. It was identified as a physical interaction partner of TgAP2XI-5 and functions to enable that factor's promoter occupancy: loss of AP2X-5 impairs AP2XI-5 binding across multiple rhoptry gene promoters genome-wide [#0, #2]. Consistent with this regulatory role, inducible knockdown of AP2X-5 reduces transcript levels of numerous virulence factors, including rhoptry and microneme genes [#1]. This transcriptional deficit has a sharply compartmentalized functional consequence: AP2X-5 disruption only mildly affects host-cell invasion in vitro but renders parasites completely avirulent in mice, establishing it as an in vivo determinant of virulence [#3].\",\n  \"teleology\": [\n    {\n      \"year\": 2018,\n      \"claim\": \"Establishing that AP2X-5 does not act alone but partners with another ApiAP2 factor defined it as a component of a cooperative transcription factor complex rather than an independent regulator.\",\n      \"evidence\": \"Co-immunoprecipitation / interaction screen identifying AP2X-5 as a binding partner of AP2XI-5 in T. gondii\",\n      \"pmids\": [\"29788176\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\"Single Co-IP without reciprocal validation or stoichiometry of the complex\", \"Whether the interaction is direct or bridged by other factors is unresolved\", \"DNA-binding specificity of AP2X-5 itself not defined\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Conditional knockdown coupled to transcriptomics answered whether AP2X-5 is functionally required for gene expression, showing it controls a defined virulence-gene program.\",\n      \"evidence\": \"Inducible conditional knockdown plus RNA-seq in T. gondii\",\n      \"pmids\": [\"29788176\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\"Does not distinguish direct AP2X-5 targets from downstream transcriptional effects\", \"Full set of dysregulated loci and their promoter architecture not detailed here\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Genome-wide ChIP of AP2XI-5 in an AP2X-5-depleted background answered the mechanistic question of how AP2X-5 acts, revealing it is required for the partner factor's promoter occupancy at virulence loci.\",\n      \"evidence\": \"Genome-wide ChIP of AP2XI-5 in the AP2X-5 knockdown strain\",\n      \"pmids\": [\"29788176\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\"Whether AP2X-5 itself binds these promoters directly not shown\", \"Mechanism by which AP2X-5 stabilizes AP2XI-5 occupancy is unknown\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Comparing in vitro invasion with in vivo mouse infection resolved the biological consequence of AP2X-5 loss, showing its requirement is specific to virulence rather than basic invasion.\",\n      \"evidence\": \"Mouse infection virulence assay and in vitro invasion assay with AP2X-5 conditional knockdown parasites\",\n      \"pmids\": [\"29788176\"],\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\"Which specific dysregulated virulence gene(s) account for the avirulence not pinpointed\", \"Effect across parasite strains/lineages not tested\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2018,\n      \"claim\": \"Expression profiling linked AP2X-5 to the S/M phase of the tachyzoite cell cycle, positioning its activity to coincide with the timing of the virulence genes it regulates.\",\n      \"evidence\": \"Expression/cell cycle staging of AP2X-5 protein and mRNA\",\n      \"pmids\": [\"29788176\"],\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"\",\n      \"gaps\": [\"Correlative timing only, without functional dissection of the cell cycle dependence\", \"Upstream regulators controlling AP2X-5 periodic expression unknown\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The intrinsic DNA-binding specificity of AP2X-5, the direct versus indirect nature of its target repertoire, and the structural basis of its cooperation with AP2XI-5 remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\"No DNA-binding motif or direct genome-wide occupancy map for AP2X-5 itself\", \"No structural model of the AP2X-5/AP2XI-5 complex\", \"Requirement across parasite strains not established\"],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0000981\", \"supporting_discovery_ids\": [0, 1, 4], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006357\", \"supporting_discovery_ids\": [1, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [\"AP2X-5 / AP2XI-5 ApiAP2 transcription factor complex\"],\n    \"partners\": [\"TgAP2XI-5\"],\n    \"other_free_text\": [\n      \"Cell cycle-regulated, with expression coordinated to the S/M phase of the tachyzoite cell cycle\",\n      \"Required for expression of rhoptry and microneme (virulence factor) genes\",\n      \"Required for AP2XI-5 promoter occupancy at rhoptry gene loci\",\n      \"Dispensable for efficient in vitro host-cell invasion but essential for virulence in a mouse infection model\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_237090","name":"AP2X5","product":"AP2 domain transcription factor AP2X-5","uniprot_acc":"S8G4N6","chromosome":"TGME49_chrX","start_pos":5702357},{"strain":"GT1","gene_id":"TGGT1_237090","name":null,"product":"AP2 domain transcription factor AP2X-5","uniprot_acc":null,"chromosome":"TGGT1_chrX","start_pos":5596510},{"strain":"RH88","gene_id":"TGRH88_040850","name":null,"product":"AP2 domain transcription factor AP2X-5","uniprot_acc":null,"chromosome":"CM023091","start_pos":1823490},{"strain":"VEG","gene_id":"TGVEG_237090","name":null,"product":"AP2 domain transcription factor AP2X-5","uniprot_acc":"V4Z6S2","chromosome":"TGVEG_chrX","start_pos":5630328}]}