{"gene":"TG_236650","gene_symbol":"DDX17","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2014,"finding":"A Toxoplasma gondii DEAD-box RNA helicase homolog, TgHoDI (TG_236650/DDX17 homolog), functions as a marker of cytoplasmic mRNA stress granules that assemble under cellular stress and translational arrest conditions; its function as a DEAD-box helicase was authenticated by complementation of a Δdhh1 yeast strain.","method":"Yeast complementation assay (Δdhh1 rescue), live parasite stress granule imaging","journal":"Gene","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — functional complementation in yeast plus localization to stress granules, single lab, two orthogonal methods","pmids":["24709106"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TgHoDI (the Toxoplasma gondii DDX17/DHH1 homolog) is a DEAD-box RNA helicase that localizes to cytoplasmic mRNA stress granules under cellular stress and translational arrest, with helicase function confirmed by complementation of a yeast Δdhh1 strain."},"narrative":{"mechanistic_narrative":"The Toxoplasma gondii DDX17 homolog (TgHoDI) is a DEAD-box RNA helicase that acts as a marker of cytoplasmic mRNA stress granules, which assemble under cellular stress and translational arrest; its helicase function was authenticated by complementation of a yeast Δdhh1 strain [PMID:24709106]. Beyond [PMID:24709106], no further mechanistic detail has been characterized in the available corpus.","teleology":[{"year":2014,"claim":"Whether the Toxoplasma DEAD-box helicase homolog was a functional helicase and where it acted in the cell was unknown; complementation and imaging established it as a genuine DEAD-box helicase that marks cytoplasmic stress granules forming under translational arrest.","evidence":"Yeast Δdhh1 complementation assay plus live parasite stress granule imaging","pmids":["24709106"],"confidence":"Medium","gaps":["Direct RNA-unwinding or ATPase activity not measured biochemically","In vivo RNA substrates and interacting partners not identified","Role in parasite gene regulation or fitness not tested by genetic perturbation"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":["Helicase function authenticated by complementation of a yeast Δdhh1 strain [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"22493121","id":"PMC_22493121","title":"Acute fatal toxoplasmosis in three Eurasian red squirrels (Sciurus vulgaris) caused by genotype II of Toxoplasma gondii.","date":"2012","source":"Journal of wildlife diseases","url":"https://pubmed.ncbi.nlm.nih.gov/22493121","citation_count":23,"is_preprint":false},{"pmid":"2278129","id":"PMC_2278129","title":"Lesions in fetal pigs with transplacentally-induced toxoplasmosis.","date":"1990","source":"Veterinary pathology","url":"https://pubmed.ncbi.nlm.nih.gov/2278129","citation_count":22,"is_preprint":false},{"pmid":"22380986","id":"PMC_22380986","title":"Potential application of serological tests on fluids from carcasses: detection of antibodies against Toxoplasma gondii and Sarcoptes scabiei in red foxes (Vulpes vulpes).","date":"2012","source":"Acta veterinaria Scandinavica","url":"https://pubmed.ncbi.nlm.nih.gov/22380986","citation_count":22,"is_preprint":false},{"pmid":"17142624","id":"PMC_17142624","title":"Role of endogenous transplacental transmission in toxoplasmosis in sheep.","date":"2006","source":"The Veterinary record","url":"https://pubmed.ncbi.nlm.nih.gov/17142624","citation_count":22,"is_preprint":false},{"pmid":"2298653","id":"PMC_2298653","title":"Toxoplasmosis and other causes of abortions in sheep from north central United States.","date":"1990","source":"Journal of the American Veterinary Medical Association","url":"https://pubmed.ncbi.nlm.nih.gov/2298653","citation_count":21,"is_preprint":false},{"pmid":"11285480","id":"PMC_11285480","title":"Vertical toxoplasmosis in a murine model. 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its function as a DEAD-box helicase was authenticated by complementation of a Δdhh1 yeast strain.\",\n      \"method\": \"Yeast complementation assay (Δdhh1 rescue), live parasite stress granule imaging\",\n      \"journal\": \"Gene\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — functional complementation in yeast plus localization to stress granules, single lab, two orthogonal methods\",\n      \"pmids\": [\"24709106\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"TgHoDI (the Toxoplasma gondii DDX17/DHH1 homolog) is a DEAD-box RNA helicase that localizes to cytoplasmic mRNA stress granules under cellular stress and translational arrest, with helicase function confirmed by complementation of a yeast Δdhh1 strain.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"The Toxoplasma gondii DDX17 homolog (TgHoDI) is a DEAD-box RNA helicase that acts as a marker of cytoplasmic mRNA stress granules, which assemble under cellular stress and translational arrest; its helicase function was authenticated by complementation of a yeast \\u0394dhh1 strain [#0]. Beyond [#0], no further mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2014,\n      \"claim\": \"Whether the Toxoplasma DEAD-box helicase homolog was a functional helicase and where it acted in the cell was unknown; complementation and imaging established it as a genuine DEAD-box helicase that marks cytoplasmic stress granules forming under translational arrest.\",\n      \"evidence\": \"Yeast \\u0394dhh1 complementation assay plus live parasite stress granule imaging\",\n      \"pmids\": [\"24709106\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Direct RNA-unwinding or ATPase activity not measured biochemically\",\n        \"In vivo RNA substrates and interacting partners not identified\",\n        \"Role in parasite gene regulation or fitness not tested by genetic perturbation\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular substrates, protein partners, regulatory triggers of stress granule recruitment, and biological consequences of TgHoDI helicase activity remain uncharacterized.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No identified RNA targets or protein interactors\",\n        \"No structural model\",\n        \"Mechanism of stress-dependent recruitment to granules unknown\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0004386\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0010494\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0034063\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Helicase function authenticated by complementation of a yeast \\u0394dhh1 strain [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_236650","name":"DDX17","product":"DEAD (Asp-Glu-Ala-Asp) box polypeptide 17","uniprot_acc":"S8EZK1","chromosome":"TGME49_chrX","start_pos":5470709},{"strain":"GT1","gene_id":"TGGT1_236650","name":null,"product":"DEAD (Asp-Glu-Ala-Asp) box polypeptide 17","uniprot_acc":null,"chromosome":"TGGT1_chrX","start_pos":5362273},{"strain":"RH88","gene_id":"TGRH88_041180","name":null,"product":"DEAD (Asp-Glu-Ala-Asp) box polypeptide 17","uniprot_acc":null,"chromosome":"CM023091","start_pos":2060850},{"strain":"VEG","gene_id":"TGVEG_236650","name":null,"product":"DEAD (Asp-Glu-Ala-Asp) box polypeptide 17","uniprot_acc":"B9PVZ6","chromosome":"TGVEG_chrX","start_pos":5396539}]}