{"gene":"TG_235380","gene_symbol":"AC5","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"AC5 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with IMC proteins as bait, localizing to a suborganellar compartment of the IMC.","method":"BioID (proximity-dependent biotin identification) in T. gondii","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Weak — BioID proximity labeling with localization, single lab, single method; AC5 identified but not individually functionally evaluated by knockout in this study","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"AC5 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling as localizing to a suborganellar compartment of the IMC, though its specific molecular function within the IMC has not yet been experimentally established."},"narrative":{"mechanistic_narrative":"AC5 is a component of the Toxoplasma gondii inner membrane complex (IMC), identified by proximity-dependent biotin identification (BioID) using IMC proteins as bait and localizing to a suborganellar compartment of the IMC, the apical cap [PMID:25691595]. Beyond this localization within the IMC, no further mechanistic detail for AC5 has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"Before this work the protein composition of the T. gondii IMC and its suborganellar sub-domains was incompletely defined; BioID established AC5 as a bona fide IMC-associated protein localizing to a discrete apical cap compartment, placing it within the parasite's pellicular cytoskeletal architecture.","evidence":"BioID proximity labeling with IMC proteins as bait in T. gondii, with localization","pmids":["25691595"],"confidence":"Medium","gaps":["Molecular function of AC5 within the IMC not experimentally established","No knockout or functional characterization of AC5 in this study","Direct binding partners and mechanism of recruitment to the apical cap unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii inner membrane complex (IMC)"],"partners":[],"other_free_text":["Identified by BioID proximity labeling using IMC proteins as bait; not individually functionally evaluated by knockout in the identifying study."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false},{"pmid":"26680740","id":"PMC_26680740","title":"An ensemble of specifically targeted proteins stabilizes cortical microtubules in the human parasite Toxoplasma gondii.","date":"2015","source":"Molecular biology of the cell","url":"https://pubmed.ncbi.nlm.nih.gov/26680740","citation_count":46,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3382,"output_tokens":581,"usd":0.00943,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5279,"output_tokens":1376,"usd":0.030398,"stage2_stop_reason":"end_turn"},"total_usd":0.03347,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"AC5 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID) with IMC proteins as bait, localizing to a suborganellar compartment of the IMC.\",\n      \"method\": \"BioID (proximity-dependent biotin identification) in T. gondii\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Weak — BioID proximity labeling with localization, single lab, single method; AC5 identified but not individually functionally evaluated by knockout in this study\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"AC5 is a novel component of the Toxoplasma gondii inner membrane complex (IMC), identified by BioID proximity labeling as localizing to a suborganellar compartment of the IMC, though its specific molecular function within the IMC has not yet been experimentally established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AC5 is a component of the Toxoplasma gondii inner membrane complex (IMC), identified by proximity-dependent biotin identification (BioID) using IMC proteins as bait and localizing to a suborganellar compartment of the IMC, the apical cap [#0]. Beyond this localization within the IMC, no further mechanistic detail for AC5 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"Before this work the protein composition of the T. gondii IMC and its suborganellar sub-domains was incompletely defined; BioID established AC5 as a bona fide IMC-associated protein localizing to a discrete apical cap compartment, placing it within the parasite's pellicular cytoskeletal architecture.\",\n      \"evidence\": \"BioID proximity labeling with IMC proteins as bait in T. gondii, with localization\",\n      \"pmids\": [\n        \"25691595\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Molecular function of AC5 within the IMC not experimentally established\",\n        \"No knockout or functional characterization of AC5 in this study\",\n        \"Direct binding partners and mechanism of recruitment to the apical cap unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of AC5 within the IMC, its interaction partners, and its contribution to parasite biology remain undetermined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional assay or genetic ablation of AC5 reported\",\n        \"No defined direct partners or complex membership\",\n        \"Role in IMC assembly, motility, or invasion unknown\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"inner membrane complex (apical cap suborganellar compartment)\",\n        \"supporting_discovery_ids\": [\n          0\n        ],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Toxoplasma gondii inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified by BioID proximity labeling using IMC proteins as bait; not individually functionally evaluated by knockout in the identifying study.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_235380","name":"AC5","product":"apical cap protein AC5","uniprot_acc":"S8EZW4","chromosome":"TGME49_chrX","start_pos":4815589},{"strain":"GT1","gene_id":"TGGT1_235380","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrX","start_pos":4705213},{"strain":"RH88","gene_id":"TGRH88_042070","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023091","start_pos":2720206},{"strain":"VEG","gene_id":"TGVEG_235380","name":null,"product":"hypothetical protein","uniprot_acc":"A0A125YYX2","chromosome":"TGVEG_chrX","start_pos":4731514}]}