{"gene":"TG_233450","gene_symbol":"SRS1","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":1997,"finding":"SRS1 (SAG1-related sequence 1) was identified as a GPI-anchored surface protein of T. gondii tachyzoites, encoded by a gene co-directionally transcribed upstream of SAG1. Antisera against recombinant SRS1 produced in E. coli recognized a specific 46 kDa band in parasite lysates corresponding to the largest GPI-anchored protein detected by Western blot.","method":"Genomic/cDNA cloning, recombinant protein expression in E. coli, Western blot, GPI-anchor analysis","journal":"Molecular and biochemical parasitology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct characterization with multiple methods (cloning, Western blot, GPI identification) in a single focused study","pmids":["9364971"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":1998,"finding":"SRS1 was confirmed as one of the GPI-anchored surface antigens dominating the tachyzoite surface, sharing conserved structural motifs (tandemly duplicated motif and 12 conserved cysteines) with SAG1 and other SAG/SRS family members, suggesting a shared secondary/tertiary structure important for a conserved function.","method":"EST database search, sequence analysis, immunofluorescence, surface expression confirmation","journal":"Infection and immunity","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — sequence analysis and immunofluorescence across multiple family members, single lab","pmids":["9573113"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2001,"finding":"Vaccination of mice with recombinant SRS1 protein (produced in E. coli) conferred partial protective immunity against lethal T. gondii challenge, with up to 25% survival and absence of brain cysts in survivors, demonstrating immunogenic potential of SRS1 in vivo.","method":"Recombinant protein immunization, lethal challenge assay, brain cyst quantification, Western blot of convalescent sera","journal":"The Journal of veterinary medical science","confidence":"Low","confidence_rationale":"Tier 3 / Weak — single lab, single method set; functional outcome (survival/cyst) without molecular mechanism defined","pmids":["11346179"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":"RH"}]},{"year":2035,"finding":"Peptides derived from polymorphic regions of SRS1 were used in a peptide microarray to detect T. gondii clonal type-specific IgY antibody responses in experimentally infected chickens and turkeys, indicating that SRS1 contains strain-polymorphic epitopes recognized by the host immune system.","method":"Synthetic peptide microarray, serology with experimentally infected poultry, ROC analysis","journal":"International journal for parasitology","confidence":"Low","confidence_rationale":"Tier 3 / Weak — indirect functional inference from serological tool development; no direct mechanistic experiment on SRS1 protein function","pmids":["29969590"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":"RH; ME49; NED"}]}],"current_model":"SRS1 (SAG1-related sequence 1) is a GPI-anchored surface protein of T. gondii tachyzoites, encoded by a gene located upstream of and co-directionally transcribed with SAG1; it shares conserved structural motifs (tandem repeat domain and 12 conserved cysteines) with other SAG/SRS family members, is expressed on the parasite surface as a ~46 kDa protein, contains strain-polymorphic epitopes recognized by the host immune system, and can elicit partial protective immunity in mice, but its precise molecular function in host-cell interaction or parasite biology remains uncharacterized."},"narrative":{"mechanistic_narrative":"SRS1 (SAG1-related sequence 1) is a GPI-anchored surface protein of Toxoplasma gondii tachyzoites, encoded by a gene positioned upstream of and co-directionally transcribed with SAG1 [PMID:9364971]. It is one of the abundant GPI-anchored antigens dominating the tachyzoite surface, migrating as a ~46 kDa band and sharing the tandemly duplicated motif and 12 conserved cysteines characteristic of the SAG/SRS family, features consistent with a conserved fold shared with SAG1 [PMID:9364971, PMID:9573113]. SRS1 carries strain-polymorphic epitopes and is immunogenic in vivo: recombinant SRS1 elicits partial protective immunity against lethal challenge in mice [PMID:11346179]. Beyond its surface localization and immunogenicity, the molecular function of SRS1 in host-cell interaction or parasite biology has not been characterized in the available corpus.","teleology":[{"year":1997,"claim":"Establishing that the SAG1-adjacent locus encodes a bona fide surface protein answered whether SRS1 is expressed and membrane-associated, defining it as a GPI-anchored tachyzoite surface antigen.","evidence":"Genomic/cDNA cloning, recombinant expression in E. coli, Western blot, and GPI-anchor analysis in T. gondii","pmids":["9364971"],"confidence":"Medium","gaps":["No molecular function or binding partner identified","Role in host-cell attachment or invasion untested","Regulation and stage-specificity of expression not resolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":1998,"claim":"Placing SRS1 within the SAG/SRS family clarified that it shares conserved structural motifs with SAG1, implying a common fold and potentially a conserved function among dominant surface antigens.","evidence":"EST database search, sequence analysis, and immunofluorescence confirming surface expression in T. gondii","pmids":["9573113"],"confidence":"Medium","gaps":["Shared structure does not establish a shared molecular activity","No structural model of SRS1 itself","Functional consequence of the conserved cysteines untested"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2001,"claim":"Immunization experiments addressed whether SRS1 is a protective antigen, showing it can elicit partial immunity but leaving its native role undefined.","evidence":"Recombinant protein immunization with lethal challenge and brain cyst quantification in mice (RH strain)","pmids":["11346179"],"confidence":"Low","gaps":["Protection is partial (up to 25% survival) and mechanism of immunity undefined","Does not reveal the parasite-intrinsic function of SRS1","Single lab, single method set"],"attribution":[{"species":"Toxoplasma gondii","strain":"RH"}]},{"year":2035,"claim":"Serological profiling established that SRS1 harbors strain-polymorphic epitopes distinguishable by host antibody responses, enabling clonal-type discrimination.","evidence":"Synthetic peptide microarray and serology in experimentally infected chickens and turkeys with ROC analysis (RH, ME49, NED strains)","pmids":["29969590"],"confidence":"Low","gaps":["Indirect inference from a diagnostic tool; no direct mechanistic experiment on SRS1 protein function","Functional significance of the polymorphisms unknown","Anomalous timeline year"],"attribution":[{"species":"Toxoplasma gondii","strain":"RH"},{"species":"Toxoplasma gondii","strain":"ME49"},{"species":"Toxoplasma gondii","strain":"NED"}]},{"year":null,"claim":"The molecular function of SRS1 — whether it mediates host-cell adhesion, invasion, or immune modulation like other SAG/SRS members — remains unresolved.","evidence":"","pmids":[],"confidence":"Low","gaps":["No ligand or host receptor identified","No knockout/knockdown phenotype reported","No direct role in attachment or invasion demonstrated"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":["SAG1 (FAMILY RELATIONSHIP AND ADJACENT CO-DIRECTIONAL GENE LOCUS, NOT A PHYSICAL INTERACTION) [#0, #1]"],"other_free_text":["GPI-anchored, ~46 kDa tachyzoite surface antigen [#0]","Member of the SAG/SRS family sharing a tandemly duplicated motif and 12 conserved cysteines with SAG1 [#1]","Contains strain-polymorphic epitopes recognized by host antibodies (RH, ME49, NED) [#3]","Recombinant SRS1 confers partial protective immunity in mice (RH challenge) [#2]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"9573113","id":"PMC_9573113","title":"The surface of Toxoplasma tachyzoites is dominated by a family of glycosylphosphatidylinositol-anchored antigens related to SAG1.","date":"1998","source":"Infection and immunity","url":"https://pubmed.ncbi.nlm.nih.gov/9573113","citation_count":116,"is_preprint":false},{"pmid":"15664946","id":"PMC_15664946","title":"Sarcocystis neurona merozoites express a family of immunogenic surface antigens that are orthologues of the Toxoplasma gondii surface antigens (SAGs) and SAG-related sequences.","date":"2005","source":"Infection and immunity","url":"https://pubmed.ncbi.nlm.nih.gov/15664946","citation_count":37,"is_preprint":false},{"pmid":"11346179","id":"PMC_11346179","title":"Modified protection against Toxoplasma gondii lethal infection and brain cyst formation by vaccination with SAG2 and SRS1.","date":"2001","source":"The Journal of veterinary medical science","url":"https://pubmed.ncbi.nlm.nih.gov/11346179","citation_count":27,"is_preprint":false},{"pmid":"9364971","id":"PMC_9364971","title":"Identification and characterization of SRS1, a Toxoplasma gondii surface antigen upstream of and related to SAG1.","date":"1997","source":"Molecular and biochemical parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/9364971","citation_count":26,"is_preprint":false},{"pmid":"24496792","id":"PMC_24496792","title":"Differential locus expansion distinguishes Toxoplasmatinae species and closely related strains of Toxoplasma gondii.","date":"2014","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/24496792","citation_count":19,"is_preprint":false},{"pmid":"32365814","id":"PMC_32365814","title":"Epidemiologic Survey on Toxoplasma gondii and Trichinella pseudospiralis Infection in Corvids from Central Italy.","date":"2020","source":"Pathogens (Basel, Switzerland)","url":"https://pubmed.ncbi.nlm.nih.gov/32365814","citation_count":12,"is_preprint":false},{"pmid":"17221136","id":"PMC_17221136","title":"Toxoplasma gondii genotyping in a dog co-infected with distemper virus and ehrlichiosis rickettsia.","date":"2006","source":"Revista do Instituto de Medicina Tropical de Sao Paulo","url":"https://pubmed.ncbi.nlm.nih.gov/17221136","citation_count":12,"is_preprint":false},{"pmid":"29969590","id":"PMC_29969590","title":"Analysis of Toxoplasma gondii clonal type-specific antibody reactions in experimentally infected turkeys and chickens.","date":"2018","source":"International journal for parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/29969590","citation_count":10,"is_preprint":false},{"pmid":"27794096","id":"PMC_27794096","title":"High frequency detection of Toxoplasma gondii DNA in human neonatal tissue from Libya.","date":"2016","source":"Transactions of the Royal Society of Tropical Medicine and Hygiene","url":"https://pubmed.ncbi.nlm.nih.gov/27794096","citation_count":10,"is_preprint":false},{"pmid":"26264261","id":"PMC_26264261","title":"Congenital toxoplasmosis and pregnancy malaria detection post-partum: Effective diagnosis and its implication for efficient management of congenital infection.","date":"2015","source":"Parasitology international","url":"https://pubmed.ncbi.nlm.nih.gov/26264261","citation_count":7,"is_preprint":false},{"pmid":"28368078","id":"PMC_28368078","title":"Genotyping of Toxoplasma gondii strain directly from human CSF samples of congenital toxoplasmosis clinical case.","date":"2017","source":"The new microbiologica","url":"https://pubmed.ncbi.nlm.nih.gov/28368078","citation_count":7,"is_preprint":false},{"pmid":"28108401","id":"PMC_28108401","title":"Constitutive expression and characterization of a surface SRS (NcSRS67) protein of Neospora caninum with no orthologue in Toxoplasma gondii.","date":"2017","source":"Parasitology international","url":"https://pubmed.ncbi.nlm.nih.gov/28108401","citation_count":6,"is_preprint":false},{"pmid":"36871500","id":"PMC_36871500","title":"In vitro anti-Toxoplasma gondii effects of a coccidiostat dinitolmide.","date":"2023","source":"Veterinary parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/36871500","citation_count":2,"is_preprint":false},{"pmid":"39160342","id":"PMC_39160342","title":"First Report on the Molecular Detection and Genetic Characterization of Toxoplasma gondii From Donkeys in Kenya.","date":"2024","source":"Acta parasitologica","url":"https://pubmed.ncbi.nlm.nih.gov/39160342","citation_count":1,"is_preprint":false},{"pmid":"24505177","id":"PMC_24505177","title":"Polymerase chain reaction based detection of Toxoplasma gondii from lymph node aspirates of dogs.","date":"2012","source":"Journal of parasitic diseases : official organ of the Indian Society for Parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/24505177","citation_count":1,"is_preprint":false},{"pmid":"33387894","id":"PMC_33387894","title":"The activity of the diaminopyrimidine dihydrofolate reducatase inhibitor, iclaprim, against Toxoplasma gondii in an in vitro model: a pilot study.","date":"2020","source":"Diagnostic microbiology and infectious disease","url":"https://pubmed.ncbi.nlm.nih.gov/33387894","citation_count":1,"is_preprint":false},{"pmid":"42188907","id":"PMC_42188907","title":"Advances and Translational Challenges in Toxoplasma gondii Vaccine Development: From Antigen Discovery to mRNA and One Health Strategies.","date":"2026","source":"Veterinary sciences","url":"https://pubmed.ncbi.nlm.nih.gov/42188907","citation_count":0,"is_preprint":false},{"pmid":"42347660","id":"PMC_42347660","title":"Protective Effect Against Acute Experimental Toxoplasmosis Conferred by Intranasal Immunisation with Toxoplasma gondii Membrane Proteins Plus CpG Adjuvant.","date":"2026","source":"Vaccines","url":"https://pubmed.ncbi.nlm.nih.gov/42347660","citation_count":0,"is_preprint":false},{"pmid":"24822344","id":"PMC_24822344","title":"[Stable green fluorescent protein expression in Toxoplasma gondii mutant].","date":"2011","source":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","url":"https://pubmed.ncbi.nlm.nih.gov/24822344","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":35442,"output_tokens":1131,"usd":0.061645,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6092,"output_tokens":2175,"usd":0.042417,"stage2_stop_reason":"end_turn"},"total_usd":0.104062,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"paper list: removed papers about other genes"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 1997,\n      \"finding\": \"SRS1 (SAG1-related sequence 1) was identified as a GPI-anchored surface protein of T. gondii tachyzoites, encoded by a gene co-directionally transcribed upstream of SAG1. Antisera against recombinant SRS1 produced in E. coli recognized a specific 46 kDa band in parasite lysates corresponding to the largest GPI-anchored protein detected by Western blot.\",\n      \"method\": \"Genomic/cDNA cloning, recombinant protein expression in E. coli, Western blot, GPI-anchor analysis\",\n      \"journal\": \"Molecular and biochemical parasitology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct characterization with multiple methods (cloning, Western blot, GPI identification) in a single focused study\",\n      \"pmids\": [\"9364971\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 1998,\n      \"finding\": \"SRS1 was confirmed as one of the GPI-anchored surface antigens dominating the tachyzoite surface, sharing conserved structural motifs (tandemly duplicated motif and 12 conserved cysteines) with SAG1 and other SAG/SRS family members, suggesting a shared secondary/tertiary structure important for a conserved function.\",\n      \"method\": \"EST database search, sequence analysis, immunofluorescence, surface expression confirmation\",\n      \"journal\": \"Infection and immunity\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — sequence analysis and immunofluorescence across multiple family members, single lab\",\n      \"pmids\": [\"9573113\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2001,\n      \"finding\": \"Vaccination of mice with recombinant SRS1 protein (produced in E. coli) conferred partial protective immunity against lethal T. gondii challenge, with up to 25% survival and absence of brain cysts in survivors, demonstrating immunogenic potential of SRS1 in vivo.\",\n      \"method\": \"Recombinant protein immunization, lethal challenge assay, brain cyst quantification, Western blot of convalescent sera\",\n      \"journal\": \"The Journal of veterinary medical science\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — single lab, single method set; functional outcome (survival/cyst) without molecular mechanism defined\",\n      \"pmids\": [\"11346179\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"RH\"}]\n    },\n    {\n      \"year\": 2035,\n      \"finding\": \"Peptides derived from polymorphic regions of SRS1 were used in a peptide microarray to detect T. gondii clonal type-specific IgY antibody responses in experimentally infected chickens and turkeys, indicating that SRS1 contains strain-polymorphic epitopes recognized by the host immune system.\",\n      \"method\": \"Synthetic peptide microarray, serology with experimentally infected poultry, ROC analysis\",\n      \"journal\": \"International journal for parasitology\",\n      \"confidence\": \"Low\",\n      \"confidence_rationale\": \"Tier 3 / Weak — indirect functional inference from serological tool development; no direct mechanistic experiment on SRS1 protein function\",\n      \"pmids\": [\"29969590\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"RH; ME49; NED\"}]\n    }\n  ],\n  \"current_model\": \"SRS1 (SAG1-related sequence 1) is a GPI-anchored surface protein of T. gondii tachyzoites, encoded by a gene located upstream of and co-directionally transcribed with SAG1; it shares conserved structural motifs (tandem repeat domain and 12 conserved cysteines) with other SAG/SRS family members, is expressed on the parasite surface as a ~46 kDa protein, contains strain-polymorphic epitopes recognized by the host immune system, and can elicit partial protective immunity in mice, but its precise molecular function in host-cell interaction or parasite biology remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"SRS1 (SAG1-related sequence 1) is a GPI-anchored surface protein of Toxoplasma gondii tachyzoites, encoded by a gene positioned upstream of and co-directionally transcribed with SAG1 [#0]. It is one of the abundant GPI-anchored antigens dominating the tachyzoite surface, migrating as a ~46 kDa band and sharing the tandemly duplicated motif and 12 conserved cysteines characteristic of the SAG/SRS family, features consistent with a conserved fold shared with SAG1 [#0, #1]. SRS1 carries strain-polymorphic epitopes and is immunogenic in vivo: recombinant SRS1 elicits partial protective immunity against lethal challenge in mice [#2]. Beyond its surface localization and immunogenicity, the molecular function of SRS1 in host-cell interaction or parasite biology has not been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 1997,\n      \"claim\": \"Establishing that the SAG1-adjacent locus encodes a bona fide surface protein answered whether SRS1 is expressed and membrane-associated, defining it as a GPI-anchored tachyzoite surface antigen.\",\n      \"evidence\": \"Genomic/cDNA cloning, recombinant expression in E. coli, Western blot, and GPI-anchor analysis in T. gondii\",\n      \"pmids\": [\"9364971\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No molecular function or binding partner identified\",\n        \"Role in host-cell attachment or invasion untested\",\n        \"Regulation and stage-specificity of expression not resolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 1998,\n      \"claim\": \"Placing SRS1 within the SAG/SRS family clarified that it shares conserved structural motifs with SAG1, implying a common fold and potentially a conserved function among dominant surface antigens.\",\n      \"evidence\": \"EST database search, sequence analysis, and immunofluorescence confirming surface expression in T. gondii\",\n      \"pmids\": [\"9573113\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Shared structure does not establish a shared molecular activity\",\n        \"No structural model of SRS1 itself\",\n        \"Functional consequence of the conserved cysteines untested\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2001,\n      \"claim\": \"Immunization experiments addressed whether SRS1 is a protective antigen, showing it can elicit partial immunity but leaving its native role undefined.\",\n      \"evidence\": \"Recombinant protein immunization with lethal challenge and brain cyst quantification in mice (RH strain)\",\n      \"pmids\": [\"11346179\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Protection is partial (up to 25% survival) and mechanism of immunity undefined\",\n        \"Does not reveal the parasite-intrinsic function of SRS1\",\n        \"Single lab, single method set\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"RH\"}]\n    },\n    {\n      \"year\": 2035,\n      \"claim\": \"Serological profiling established that SRS1 harbors strain-polymorphic epitopes distinguishable by host antibody responses, enabling clonal-type discrimination.\",\n      \"evidence\": \"Synthetic peptide microarray and serology in experimentally infected chickens and turkeys with ROC analysis (RH, ME49, NED strains)\",\n      \"pmids\": [\"29969590\"],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"Indirect inference from a diagnostic tool; no direct mechanistic experiment on SRS1 protein function\",\n        \"Functional significance of the polymorphisms unknown\",\n        \"Anomalous timeline year\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"RH\"},\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"ME49\"},\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"NED\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular function of SRS1 — whether it mediates host-cell adhesion, invasion, or immune modulation like other SAG/SRS members — remains unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Low\",\n      \"gaps\": [\n        \"No ligand or host receptor identified\",\n        \"No knockout/knockdown phenotype reported\",\n        \"No direct role in attachment or invasion demonstrated\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0038023\", \"supporting_discovery_ids\": [2, 3], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0009986\", \"supporting_discovery_ids\": [0, 1], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0098552\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [\"SAG1 (family relationship and adjacent co-directional gene locus, not a physical interaction) [#0, #1]\"],\n    \"other_free_text\": [\n      \"GPI-anchored, ~46 kDa tachyzoite surface antigen [#0]\",\n      \"Member of the SAG/SRS family sharing a tandemly duplicated motif and 12 conserved cysteines with SAG1 [#1]\",\n      \"Contains strain-polymorphic epitopes recognized by host antibodies (RH, ME49, NED) [#3]\",\n      \"Recombinant SRS1 confers partial protective immunity in mice (RH challenge) [#2]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_233450","name":"SRS1","product":"SAG-related sequence SRS29A","uniprot_acc":"S8GAG4","chromosome":"TGME49_chrVIII","start_pos":2660930},{"strain":"GT1","gene_id":"TGGT1_233450","name":"SRS1","product":"SAG-related sequence SRS29A","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":2656903},{"strain":"RH88","gene_id":"TGRH88_077440","name":null,"product":"SAG-related sequence SRS29A","uniprot_acc":null,"chromosome":"CM023089","start_pos":7767262},{"strain":"VEG","gene_id":"TGVEG_233450","name":"SRS1","product":"SAG-related sequence SRS29A","uniprot_acc":"B9Q4G2","chromosome":"TGVEG_chrVIII","start_pos":2669490}]}