{"gene":"TG_231840","gene_symbol":"PCR3","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"The preconoidal rings (PCRs), which include PCR3 (TG_231840) as part of their composition, serve as a hub for glideosome components including Formin1, as established by ultrastructure expansion microscopy.","method":"Ultrastructure expansion microscopy (U-ExM)","journal":"Nature microbiology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct localization experiment with functional consequence using U-ExM in a single rigorous study; PCR3 is a preconoidal ring component but the abstract does not detail PCR3-specific experiments separately from the broader PCR complex","pmids":["36109645"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"PCR3 is a component of the preconoidal rings (PCRs) in Toxoplasma gondii, structures that function as a hub for glideosome components including Formin1, linking the PCRs to actomyosin-dependent gliding motility and invasion; however, specific molecular mechanisms or direct binding partners for PCR3 itself have not yet been experimentally established."},"narrative":{"mechanistic_narrative":"PCR3 is a component of the preconoidal rings (PCRs) of Toxoplasma gondii, apical cytoskeletal structures that serve as a hub for glideosome components including Formin1, thereby linking the PCRs to the actomyosin machinery underlying gliding motility and invasion [PMID:36109645]. Beyond its identification as a PCR constituent, no PCR3-specific molecular mechanism or direct binding partner has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Whether the preconoidal rings organize glideosome components was unknown; imaging the apical complex at high resolution established that the PCRs—of which PCR3 is a compositional member—act as a hub concentrating glideosome factors such as Formin1.","evidence":"Ultrastructure expansion microscopy (U-ExM) in Toxoplasma gondii","pmids":["36109645"],"confidence":"Medium","gaps":["No PCR3-specific experiments distinguish its role from the broader PCR complex","No direct binding partner of PCR3 has been identified","The molecular contribution of PCR3 to Formin1 recruitment or motility is undefined"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Preconoidal rings (PCRs)"],"partners":[],"other_free_text":["PCRs serve as a hub for glideosome components including Formin1 [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"36109645","id":"PMC_36109645","title":"Conoid extrusion regulates glideosome assembly to control motility and invasion in Apicomplexa.","date":"2022","source":"Nature microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/36109645","citation_count":37,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2848,"output_tokens":932,"usd":0.011262,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5800,"output_tokens":1900,"usd":0.03825,"stage2_stop_reason":"end_turn"},"total_usd":0.083264,"stage1_batch_id":"msgbatch_01TEPNZ4MCzJ7xtsNwosiDRk","stage2_batch_id":"msgbatch_01FQShM7n1sfCr4pWTDShmki","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"The preconoidal rings (PCRs), which include PCR3 (TG_231840) as part of their composition, serve as a hub for glideosome components including Formin1, as established by ultrastructure expansion microscopy.\",\n      \"method\": \"Ultrastructure expansion microscopy (U-ExM)\",\n      \"journal\": \"Nature microbiology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct localization experiment with functional consequence using U-ExM in a single rigorous study; PCR3 is a preconoidal ring component but the abstract does not detail PCR3-specific experiments separately from the broader PCR complex\",\n      \"pmids\": [\"36109645\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"PCR3 is a component of the preconoidal rings (PCRs) in Toxoplasma gondii, structures that function as a hub for glideosome components including Formin1, linking the PCRs to actomyosin-dependent gliding motility and invasion; however, specific molecular mechanisms or direct binding partners for PCR3 itself have not yet been experimentally established.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"PCR3 is a component of the preconoidal rings (PCRs) of Toxoplasma gondii, apical cytoskeletal structures that serve as a hub for glideosome components including Formin1, thereby linking the PCRs to the actomyosin machinery underlying gliding motility and invasion [#0]. Beyond its identification as a PCR constituent, no PCR3-specific molecular mechanism or direct binding partner has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Whether the preconoidal rings organize glideosome components was unknown; imaging the apical complex at high resolution established that the PCRs—of which PCR3 is a compositional member—act as a hub concentrating glideosome factors such as Formin1.\",\n      \"evidence\": \"Ultrastructure expansion microscopy (U-ExM) in Toxoplasma gondii\",\n      \"pmids\": [\n        \"36109645\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No PCR3-specific experiments distinguish its role from the broader PCR complex\",\n        \"No direct binding partner of PCR3 has been identified\",\n        \"The molecular contribution of PCR3 to Formin1 recruitment or motility is undefined\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"It remains unknown what specific molecular function PCR3 performs within the preconoidal rings and which partners it directly binds.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No direct interaction partner established for PCR3\",\n        \"No functional or knockout phenotype attributed to PCR3 alone\",\n        \"No structural model of PCR3 within the PCR\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"preconoidal rings\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"gliding motility / host cell invasion (glideosome)\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"Preconoidal rings (PCRs)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"PCRs serve as a hub for glideosome components including Formin1 [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_231840","name":"PCR3","product":"preconoidal region protein PCR3","uniprot_acc":"A0A125YG78","chromosome":"TGME49_chrVIII","start_pos":1578009},{"strain":"GT1","gene_id":"TGGT1_231840","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":1574106},{"strain":"RH88","gene_id":"TGRH88_075890","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":6684880},{"strain":"VEG","gene_id":"TGVEG_231840","name":null,"product":"putative tola protein","uniprot_acc":"B6KJN2","chromosome":"TGVEG_chrVIII","start_pos":1584912}]}