{"gene":"TG_227900","gene_symbol":"AP2X1","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2025,"finding":"AP2X-1 is expressed during tachyzoite and bradyzoite stages but decreases in the mature merozoite stage; knockout of ap2X-1 significantly impaired tachyzoite invasion and replication while increasing the frequency of bradyzoite differentiation.","method":"CRISPR-based gene knockout, phenotypic assays (invasion, replication), immunofluorescence","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — clean KO with defined cellular phenotypes (invasion, replication, differentiation), single lab with multiple orthogonal phenotypic readouts","pmids":["40823841"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"finding":"AP2X-1 physically associates with the HDAC3/MORC repressor complex, and loss of ap2X-1 leads to upregulation of bradyzoite- and sexual stage-specific genes, indicating AP2X-1 functions as a transcriptional repressor of these gene sets through this complex.","method":"Co-immunoprecipitation/complex association assay, RNA-seq transcriptomics after knockout","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — complex association demonstrated and corroborated by transcriptomic changes, single lab","pmids":["40823841"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"finding":"CUT&TAG analysis revealed substantial overlap between AP2X-1 genomic binding and HDAC3/MORC complex occupancy at promoters of bradyzoite- and sexual stage-specific genes, and ATAC-seq demonstrated that AP2X-1 influences chromatin compaction and accessibility at these loci.","method":"CUT&TAG (Cleavage Under Targets and Tagmentation), ATAC-seq","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — two orthogonal genome-wide chromatin methods in single lab establishing direct promoter binding and chromatin remodeling function","pmids":["40823841"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"finding":"Single-cell RNA sequencing of the ap2X-1 knockout strain revealed a mixed population including tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like parasites, indicating AP2X-1 is a critical negative regulator of sexual commitment and stage transitions.","method":"Single-cell RNA sequencing (scRNA-seq) of knockout parasites","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — single-cell transcriptomics with KO, single lab, multiple stage identities detected","pmids":["40823841"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"finding":"Loss of ap2X-1 resulted in significant attenuation of T. gondii virulence and decreased brain cyst formation in vivo, consistent with its role in repressing bradyzoite differentiation and sexual commitment.","method":"Mouse infection model (in vivo virulence assay, brain cyst counting)","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — in vivo KO phenotype with defined readouts (virulence, cyst burden), single lab","pmids":["40823841"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"AP2X-1 is a nuclear transcription factor expressed in tachyzoites and bradyzoites that associates with the HDAC3/MORC repressor complex and binds promoters of bradyzoite- and sexual stage-specific genes to repress their expression, thereby suppressing bradyzoite differentiation and sexual commitment; loss of AP2X-1 impairs tachyzoite invasion and replication, derepresses stage-specific gene programs, promotes chromatin accessibility at sexual-stage loci, and attenuates virulence and brain cyst formation in vivo."},"narrative":{"mechanistic_narrative":"AP2X-1 is a nuclear ApiAP2-family transcriptional repressor in Toxoplasma gondii that suppresses stage differentiation and sexual commitment to maintain the proliferative tachyzoite program [PMID:40823841]. Expressed in tachyzoites and bradyzoites but downregulated in mature merozoites, AP2X-1 is required for efficient tachyzoite invasion and replication, and its loss increases bradyzoite differentiation [PMID:40823841]. It acts through physical association with the HDAC3/MORC repressor complex, and genome-wide binding overlaps HDAC3/MORC occupancy at promoters of bradyzoite- and sexual stage-specific genes, where AP2X-1 promotes chromatin compaction and restricts accessibility [PMID:40823841]. Consequent to this repression, knockout parasites derepress bradyzoite- and sexual-stage gene programs and give rise to a mixed population of tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like cells, identifying AP2X-1 as a critical negative regulator of stage transitions [PMID:40823841]. In vivo, loss of AP2X-1 attenuates virulence and reduces brain cyst formation, linking its repressive function to pathogenesis [PMID:40823841].","teleology":[{"year":2025,"claim":"Whether AP2X-1 contributes to tachyzoite fitness and stage identity was unknown; knockout established it is required for invasion and replication and normally restrains bradyzoite differentiation.","evidence":"CRISPR knockout with invasion, replication, and differentiation phenotyping and immunofluorescence in T. gondii","pmids":["40823841"],"confidence":"Medium","gaps":["Does not define which direct target genes drive the invasion/replication defect","Strain background not specified in the attribution"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"claim":"The molecular mechanism of repression was unknown; AP2X-1 was shown to associate with the HDAC3/MORC repressor complex and its loss derepresses bradyzoite- and sexual stage-specific genes, establishing it as a transcriptional repressor acting through this complex.","evidence":"Co-immunoprecipitation/complex association plus RNA-seq of knockout parasites in T. gondii","pmids":["40823841"],"confidence":"Medium","gaps":["Interaction shown by complex association without reciprocal or structural validation of the AP2X-1–HDAC3/MORC contact","Whether AP2X-1 recruits the complex or vice versa is unresolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"claim":"Whether AP2X-1 binds target promoters directly and remodels chromatin was open; CUT&TAG and ATAC-seq showed binding overlapping HDAC3/MORC occupancy at stage-specific promoters and a role in chromatin compaction/accessibility.","evidence":"CUT&TAG genomic occupancy and ATAC-seq chromatin accessibility profiling in T. gondii","pmids":["40823841"],"confidence":"Medium","gaps":["DNA sequence motif recognized by AP2X-1 not defined","Direct causality between binding and compaction versus indirect effects not dissected"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"claim":"The breadth of stage programs controlled by AP2X-1 was unclear; single-cell RNA-seq of the knockout revealed emergence of tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like cells, establishing AP2X-1 as a negative regulator of sexual commitment and stage transitions.","evidence":"Single-cell RNA sequencing of knockout T. gondii","pmids":["40823841"],"confidence":"Medium","gaps":["Does not establish whether derepressed cells are functionally competent for the full sexual cycle","Temporal ordering of stage-fate decisions not resolved"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2025,"claim":"Whether the repressive function affects pathogenesis was unknown; knockout attenuated virulence and decreased brain cyst formation in mice, linking AP2X-1 repression to in vivo disease.","evidence":"Mouse infection model with virulence and brain cyst quantification","pmids":["40823841"],"confidence":"Medium","gaps":["Does not separate the contribution of invasion/replication defects from stage-derepression to attenuation","Single-lab in vivo result"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The DNA-binding specificity of AP2X-1 and the direction of recruitment within the HDAC3/MORC complex remain undefined.","evidence":"","pmids":[],"confidence":"Medium","gaps":["No AP2X-1 binding motif reported","No structural or reciprocal validation of the HDAC3/MORC association","Mechanism of complex recruitment to stage-specific promoters unknown"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["HDAC3/MORC repressor complex"],"partners":["HDAC3","MORC"],"other_free_text":["Expressed in tachyzoites and bradyzoites, downregulated in mature merozoites [#0]","Required for tachyzoite invasion and replication; knockout increases bradyzoite differentiation [#0]","Binds promoters of bradyzoite- and sexual stage-specific genes and promotes chromatin compaction [#2]","Knockout yields mixed tachyzoite/bradyzoite/merozoite/sporozoite-like populations, indicating negative regulation of sexual commitment [#3]","Loss attenuates virulence and reduces brain cyst formation in mice [#4]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"20865045","id":"PMC_20865045","title":"Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.","date":"2010","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/20865045","citation_count":234,"is_preprint":false},{"pmid":"29932347","id":"PMC_29932347","title":"The Epigenome, Cell Cycle, and Development in Toxoplasma.","date":"2018","source":"Annual review of microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/29932347","citation_count":51,"is_preprint":false},{"pmid":"20657661","id":"PMC_20657661","title":"The construction and use of log-odds substitution scores for multiple sequence alignment.","date":"2010","source":"PLoS computational biology","url":"https://pubmed.ncbi.nlm.nih.gov/20657661","citation_count":48,"is_preprint":false},{"pmid":"22486860","id":"PMC_22486860","title":"Cactin is essential for G1 progression in Toxoplasma gondii.","date":"2012","source":"Molecular microbiology","url":"https://pubmed.ncbi.nlm.nih.gov/22486860","citation_count":23,"is_preprint":false},{"pmid":"25393307","id":"PMC_25393307","title":"Distinct strains of Toxoplasma gondii feature divergent transcriptomes regardless of developmental stage.","date":"2014","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/25393307","citation_count":22,"is_preprint":false},{"pmid":"18983845","id":"PMC_18983845","title":"New eukaryotic systematics: a phylogenetic perspective of developmental gene expression in the Apicomplexa.","date":"2008","source":"International journal for parasitology","url":"https://pubmed.ncbi.nlm.nih.gov/18983845","citation_count":12,"is_preprint":false},{"pmid":"39690155","id":"PMC_39690155","title":"Apicomplexan mitoribosome from highly fragmented rRNAs to a functional machine.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/39690155","citation_count":9,"is_preprint":false},{"pmid":"36178892","id":"PMC_36178892","title":"Genomic insights into host and parasite interactions during intracellular infection by Toxoplasma gondii.","date":"2022","source":"PloS one","url":"https://pubmed.ncbi.nlm.nih.gov/36178892","citation_count":2,"is_preprint":false},{"pmid":"40823841","id":"PMC_40823841","title":"AP2X-1 is a negative regulator of Toxoplasma gondii sexual commitment.","date":"2025","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/40823841","citation_count":1,"is_preprint":false},{"pmid":"42087241","id":"PMC_42087241","title":"Host-cell dependent epigenetic profiles associated with survival outcomes in T. gondii infection.","date":"2026","source":"Epigenetics & chromatin","url":"https://pubmed.ncbi.nlm.nih.gov/42087241","citation_count":0,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":18746,"output_tokens":1392,"usd":0.038559,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":6295,"output_tokens":2778,"usd":0.050463,"stage2_stop_reason":"end_turn"},"total_usd":0.089022,"stage1_batch_id":"msgbatch_01WokvBYTB6JLnYrpD4rXnyV","stage2_batch_id":"msgbatch_01M7hsQDvz6AqVC9DLVjQzzc","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"paper list: removed papers about other genes"},"stage1_raw":"Looking at the corpus, I need to identify papers specifically about AP2X-1 (also written as AP2X1). The key paper is PMID:40823841 (paper [27]), which directly studies AP2X-1 in Toxoplasma gondii. Let me also check for any other relevant papers about this specific factor.\n\nPaper [27] is the primary mechanistic paper about AP2X-1. Papers [1]-[26] and [28]-[32] are about other AP2 factors or general surveys. Paper [32] mentions AP2X-8 in a genomic context only. I'll extract discoveries only from paper [27].\n\n```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2025,\n      \"finding\": \"AP2X-1 is expressed during tachyzoite and bradyzoite stages but decreases in the mature merozoite stage; knockout of ap2X-1 significantly impaired tachyzoite invasion and replication while increasing the frequency of bradyzoite differentiation.\",\n      \"method\": \"CRISPR-based gene knockout, phenotypic assays (invasion, replication), immunofluorescence\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean KO with defined cellular phenotypes (invasion, replication, differentiation), single lab with multiple orthogonal phenotypic readouts\",\n      \"pmids\": [\"40823841\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"AP2X-1 physically associates with the HDAC3/MORC repressor complex, and loss of ap2X-1 leads to upregulation of bradyzoite- and sexual stage-specific genes, indicating AP2X-1 functions as a transcriptional repressor of these gene sets through this complex.\",\n      \"method\": \"Co-immunoprecipitation/complex association assay, RNA-seq transcriptomics after knockout\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — complex association demonstrated and corroborated by transcriptomic changes, single lab\",\n      \"pmids\": [\"40823841\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"CUT&TAG analysis revealed substantial overlap between AP2X-1 genomic binding and HDAC3/MORC complex occupancy at promoters of bradyzoite- and sexual stage-specific genes, and ATAC-seq demonstrated that AP2X-1 influences chromatin compaction and accessibility at these loci.\",\n      \"method\": \"CUT&TAG (Cleavage Under Targets and Tagmentation), ATAC-seq\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — two orthogonal genome-wide chromatin methods in single lab establishing direct promoter binding and chromatin remodeling function\",\n      \"pmids\": [\"40823841\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Single-cell RNA sequencing of the ap2X-1 knockout strain revealed a mixed population including tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like parasites, indicating AP2X-1 is a critical negative regulator of sexual commitment and stage transitions.\",\n      \"method\": \"Single-cell RNA sequencing (scRNA-seq) of knockout parasites\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — single-cell transcriptomics with KO, single lab, multiple stage identities detected\",\n      \"pmids\": [\"40823841\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"finding\": \"Loss of ap2X-1 resulted in significant attenuation of T. gondii virulence and decreased brain cyst formation in vivo, consistent with its role in repressing bradyzoite differentiation and sexual commitment.\",\n      \"method\": \"Mouse infection model (in vivo virulence assay, brain cyst counting)\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — in vivo KO phenotype with defined readouts (virulence, cyst burden), single lab\",\n      \"pmids\": [\"40823841\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"AP2X-1 is a nuclear transcription factor expressed in tachyzoites and bradyzoites that associates with the HDAC3/MORC repressor complex and binds promoters of bradyzoite- and sexual stage-specific genes to repress their expression, thereby suppressing bradyzoite differentiation and sexual commitment; loss of AP2X-1 impairs tachyzoite invasion and replication, derepresses stage-specific gene programs, promotes chromatin accessibility at sexual-stage loci, and attenuates virulence and brain cyst formation in vivo.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"AP2X-1 is a nuclear ApiAP2-family transcriptional repressor in Toxoplasma gondii that suppresses stage differentiation and sexual commitment to maintain the proliferative tachyzoite program [#0, #3]. Expressed in tachyzoites and bradyzoites but downregulated in mature merozoites, AP2X-1 is required for efficient tachyzoite invasion and replication, and its loss increases bradyzoite differentiation [#0]. It acts through physical association with the HDAC3/MORC repressor complex, and genome-wide binding overlaps HDAC3/MORC occupancy at promoters of bradyzoite- and sexual stage-specific genes, where AP2X-1 promotes chromatin compaction and restricts accessibility [#1, #2]. Consequent to this repression, knockout parasites derepress bradyzoite- and sexual-stage gene programs and give rise to a mixed population of tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like cells, identifying AP2X-1 as a critical negative regulator of stage transitions [#1, #3]. In vivo, loss of AP2X-1 attenuates virulence and reduces brain cyst formation, linking its repressive function to pathogenesis [#4].\",\n  \"teleology\": [\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether AP2X-1 contributes to tachyzoite fitness and stage identity was unknown; knockout established it is required for invasion and replication and normally restrains bradyzoite differentiation.\",\n      \"evidence\": \"CRISPR knockout with invasion, replication, and differentiation phenotyping and immunofluorescence in T. gondii\",\n      \"pmids\": [\"40823841\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not define which direct target genes drive the invasion/replication defect\",\n        \"Strain background not specified in the attribution\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"The molecular mechanism of repression was unknown; AP2X-1 was shown to associate with the HDAC3/MORC repressor complex and its loss derepresses bradyzoite- and sexual stage-specific genes, establishing it as a transcriptional repressor acting through this complex.\",\n      \"evidence\": \"Co-immunoprecipitation/complex association plus RNA-seq of knockout parasites in T. gondii\",\n      \"pmids\": [\"40823841\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Interaction shown by complex association without reciprocal or structural validation of the AP2X-1–HDAC3/MORC contact\",\n        \"Whether AP2X-1 recruits the complex or vice versa is unresolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether AP2X-1 binds target promoters directly and remodels chromatin was open; CUT&TAG and ATAC-seq showed binding overlapping HDAC3/MORC occupancy at stage-specific promoters and a role in chromatin compaction/accessibility.\",\n      \"evidence\": \"CUT&TAG genomic occupancy and ATAC-seq chromatin accessibility profiling in T. gondii\",\n      \"pmids\": [\"40823841\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"DNA sequence motif recognized by AP2X-1 not defined\",\n        \"Direct causality between binding and compaction versus indirect effects not dissected\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"The breadth of stage programs controlled by AP2X-1 was unclear; single-cell RNA-seq of the knockout revealed emergence of tachyzoite-, bradyzoite-, merozoite-, and sporozoite-like cells, establishing AP2X-1 as a negative regulator of sexual commitment and stage transitions.\",\n      \"evidence\": \"Single-cell RNA sequencing of knockout T. gondii\",\n      \"pmids\": [\"40823841\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not establish whether derepressed cells are functionally competent for the full sexual cycle\",\n        \"Temporal ordering of stage-fate decisions not resolved\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2025,\n      \"claim\": \"Whether the repressive function affects pathogenesis was unknown; knockout attenuated virulence and decreased brain cyst formation in mice, linking AP2X-1 repression to in vivo disease.\",\n      \"evidence\": \"Mouse infection model with virulence and brain cyst quantification\",\n      \"pmids\": [\"40823841\"],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Does not separate the contribution of invasion/replication defects from stage-derepression to attenuation\",\n        \"Single-lab in vivo result\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The DNA-binding specificity of AP2X-1 and the direction of recruitment within the HDAC3/MORC complex remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No AP2X-1 binding motif reported\",\n        \"No structural or reciprocal validation of the HDAC3/MORC association\",\n        \"Mechanism of complex recruitment to stage-specific promoters unknown\"\n      ],\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\"term_id\": \"GO:0000122\", \"supporting_discovery_ids\": [1, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0003700\", \"supporting_discovery_ids\": [1, 2, 3], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0000976\", \"supporting_discovery_ids\": [2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"localization\": [\n      {\"term_id\": \"GO:0005634\", \"supporting_discovery_ids\": [1, 2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0006338\", \"supporting_discovery_ids\": [2], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]},\n      {\"term_id\": \"GO:0030154\", \"supporting_discovery_ids\": [0, 3], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [\n      \"HDAC3/MORC repressor complex\"\n    ],\n    \"partners\": [\n      \"HDAC3\",\n      \"MORC\"\n    ],\n    \"other_free_text\": [\n      \"Expressed in tachyzoites and bradyzoites, downregulated in mature merozoites [#0]\",\n      \"Required for tachyzoite invasion and replication; knockout increases bradyzoite differentiation [#0]\",\n      \"Binds promoters of bradyzoite- and sexual stage-specific genes and promotes chromatin compaction [#2]\",\n      \"Knockout yields mixed tachyzoite/bradyzoite/merozoite/sporozoite-like populations, indicating negative regulation of sexual commitment [#3]\",\n      \"Loss attenuates virulence and reduces brain cyst formation in mice [#4]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_227900","name":"AP2X1","product":"AP2 domain transcription factor AP2X-1","uniprot_acc":"S8G6D4","chromosome":"TGME49_chrX","start_pos":744093},{"strain":"GT1","gene_id":"TGGT1_227900","name":null,"product":"AP2 domain transcription factor AP2X-1","uniprot_acc":null,"chromosome":"TGGT1_chrX","start_pos":654907},{"strain":"RH88","gene_id":"TGRH88_047220","name":null,"product":"AP2 domain transcription factor AP2X-1","uniprot_acc":null,"chromosome":"CM023091","start_pos":6820859},{"strain":"VEG","gene_id":"TGVEG_227900","name":null,"product":"AP2 domain transcription factor AP2X-1","uniprot_acc":"V4ZKE7","chromosome":"TGVEG_chrX","start_pos":634732}]}