{"gene":"TG_224700","gene_symbol":"ICMAP2","run_date":"2026-09-08T11:06:42","timeline":{"discoveries":[{"year":2024,"finding":"Depletion of ICMAP2 causes dissociation of intraconoidal microtubules (ICMTs), their detachment from the conoid, and dispersion of microtubule-associated vesicles (MVs) and rhoptries, demonstrating that ICMAP2 is required for maintaining ICMT integrity and scaffolding rhoptry positioning for iterative discharge.","method":"Conditional depletion (knockdown) of ICMAP2 with ultrastructural and fluorescence imaging readouts of ICMT organization, conoid attachment, and rhoptry/MV localization","journal":"Nature communications","confidence":"High","confidence_rationale":"Tier 2 / Moderate — clean loss-of-function with defined cellular phenotype (ICMT dissociation, conoid detachment, rhoptry dispersion) using direct localization/ultrastructural imaging in a single rigorous study","pmids":["38191574"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"ICMAP2 is an intraconoidal microtubule-associated protein in Toxoplasma gondii that maintains ICMT cohesion and their attachment to the conoid, thereby scaffolding the positioning of microtubule-associated vesicles and rhoptries for sustained, iterative rhoptry discharge during invasion."},"narrative":{"mechanistic_narrative":"ICMAP2 is an intraconoidal microtubule-associated protein in Toxoplasma gondii required to maintain the integrity of the intraconoidal microtubules (ICMTs) and their attachment to the conoid [PMID:38191574]. Conditional depletion of ICMAP2 causes the ICMTs to dissociate and detach from the conoid, with concomitant dispersion of microtubule-associated vesicles and rhoptries, establishing that ICMAP2 scaffolds the positioning of these organelles for sustained, iterative rhoptry discharge during invasion [PMID:38191574]. Beyond this single loss-of-function characterization, no further molecular detail — including direct binding partners, structural basis of ICMT association, or biochemical activity — has been characterized in the available corpus.","teleology":[{"year":2024,"claim":"It was unknown what maintains intraconoidal microtubule cohesion and anchors them to the conoid; conditional depletion showed ICMAP2 is required for ICMT integrity and for scaffolding the positioning of microtubule-associated vesicles and rhoptries, establishing its role in enabling iterative rhoptry discharge.","evidence":"Conditional knockdown of ICMAP2 with ultrastructural and fluorescence imaging of ICMT organization, conoid attachment, and rhoptry/MV localization in Toxoplasma gondii","pmids":["38191574"],"confidence":"High","gaps":["No direct binding partners or molecular basis of ICMAP2-ICMT association identified","No structural model of how ICMAP2 tethers ICMTs to the conoid","Mechanism linking ICMT integrity to rhoptry discharge not resolved biochemically"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The molecular partners, structural basis of microtubule binding, and biochemical activity of ICMAP2 remain undefined.","evidence":"","pmids":[],"confidence":"High","gaps":["No interactome or reciprocal binding validation for ICMAP2","No domain-level or structural characterization","No mechanism for how ICMAP2 loss propagates to rhoptry mispositioning"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":[],"partners":[],"other_free_text":["Intraconoidal microtubule-associated protein required for ICMT integrity and attachment to the conoid [#0]","Scaffolds positioning of microtubule-associated vesicles and rhoptries for iterative rhoptry discharge during invasion [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"38191574","id":"PMC_38191574","title":"Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins.","date":"2024","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/38191574","citation_count":16,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2848,"output_tokens":393,"usd":0.00722,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5008,"output_tokens":1078,"usd":0.025995,"stage2_stop_reason":"end_turn"},"total_usd":0.033215,"stage1_batch_id":"msgbatch_01WokvBYTB6JLnYrpD4rXnyV","stage2_batch_id":"msgbatch_01M7hsQDvz6AqVC9DLVjQzzc","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2024,\n      \"finding\": \"Depletion of ICMAP2 causes dissociation of intraconoidal microtubules (ICMTs), their detachment from the conoid, and dispersion of microtubule-associated vesicles (MVs) and rhoptries, demonstrating that ICMAP2 is required for maintaining ICMT integrity and scaffolding rhoptry positioning for iterative discharge.\",\n      \"method\": \"Conditional depletion (knockdown) of ICMAP2 with ultrastructural and fluorescence imaging readouts of ICMT organization, conoid attachment, and rhoptry/MV localization\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"High\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — clean loss-of-function with defined cellular phenotype (ICMT dissociation, conoid detachment, rhoptry dispersion) using direct localization/ultrastructural imaging in a single rigorous study\",\n      \"pmids\": [\"38191574\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"ICMAP2 is an intraconoidal microtubule-associated protein in Toxoplasma gondii that maintains ICMT cohesion and their attachment to the conoid, thereby scaffolding the positioning of microtubule-associated vesicles and rhoptries for sustained, iterative rhoptry discharge during invasion.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"ICMAP2 is an intraconoidal microtubule-associated protein in Toxoplasma gondii required to maintain the integrity of the intraconoidal microtubules (ICMTs) and their attachment to the conoid [#0]. Conditional depletion of ICMAP2 causes the ICMTs to dissociate and detach from the conoid, with concomitant dispersion of microtubule-associated vesicles and rhoptries, establishing that ICMAP2 scaffolds the positioning of these organelles for sustained, iterative rhoptry discharge during invasion [#0]. Beyond this single loss-of-function characterization, no further molecular detail — including direct binding partners, structural basis of ICMT association, or biochemical activity — has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2024,\n      \"claim\": \"It was unknown what maintains intraconoidal microtubule cohesion and anchors them to the conoid; conditional depletion showed ICMAP2 is required for ICMT integrity and for scaffolding the positioning of microtubule-associated vesicles and rhoptries, establishing its role in enabling iterative rhoptry discharge.\",\n      \"evidence\": \"Conditional knockdown of ICMAP2 with ultrastructural and fluorescence imaging of ICMT organization, conoid attachment, and rhoptry/MV localization in Toxoplasma gondii\",\n      \"pmids\": [\n        \"38191574\"\n      ],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"No direct binding partners or molecular basis of ICMAP2-ICMT association identified\",\n        \"No structural model of how ICMAP2 tethers ICMTs to the conoid\",\n        \"Mechanism linking ICMT integrity to rhoptry discharge not resolved biochemically\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular partners, structural basis of microtubule binding, and biochemical activity of ICMAP2 remain undefined.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"High\",\n      \"gaps\": [\n        \"No interactome or reciprocal binding validation for ICMAP2\",\n        \"No domain-level or structural characterization\",\n        \"No mechanism for how ICMAP2 loss propagates to rhoptry mispositioning\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [],\n    \"pathway\": [],\n    \"complexes\": [],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Intraconoidal microtubule-associated protein required for ICMT integrity and attachment to the conoid [#0]\",\n      \"Scaffolds positioning of microtubule-associated vesicles and rhoptries for iterative rhoptry discharge during invasion [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_224700","name":"ICMAP2","product":"intraconoidal microtubule-associated protein ICMAP2","uniprot_acc":"S8F0H4","chromosome":"TGME49_chrX","start_pos":2865450},{"strain":"GT1","gene_id":"TGGT1_224700","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrX","start_pos":2774904},{"strain":"RH88","gene_id":"TGRH88_044350","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023091","start_pos":4698069},{"strain":"VEG","gene_id":"TGVEG_224700","name":null,"product":"hypothetical protein","uniprot_acc":"V4YWG1","chromosome":"TGVEG_chrX","start_pos":2764633}]}