{"gene":"TG_217510","gene_symbol":"IMC19","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2015,"finding":"IMC19 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID), localizing to a specific suborganellar compartment of the IMC (alveoli or cytoskeletal network).","method":"BioID proximity labeling in T. gondii, followed by mass spectrometry identification","journal":"mBio","confidence":"Medium","confidence_rationale":"Tier 3 / Moderate — BioID proximity labeling with MS identification in a single study; localization established but limited functional characterization specific to IMC19","pmids":["25691595"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"IMC19 is a novel component of the Toxoplasma gondii inner membrane complex, identified by BioID proximity labeling, and resides within the IMC's alveolar or cytoskeletal subcompartments, though its specific molecular function remains uncharacterized."},"narrative":{"mechanistic_narrative":"IMC19 is a component of the inner membrane complex (IMC) of Toxoplasma gondii, identified through BioID proximity labeling and localizing to a specific suborganellar subcompartment of the IMC, either its alveolar membranes or the underlying cytoskeletal network [PMID:25691595]. Beyond this identification and localization, no further mechanistic detail about IMC19 has been characterized in the available corpus.","teleology":[{"year":2015,"claim":"The composition of the Toxoplasma IMC was incompletely defined; applying BioID proximity labeling to the IMC established IMC19 as a bona fide novel constituent and placed it within a defined IMC subcompartment.","evidence":"BioID proximity labeling followed by mass spectrometry identification in T. gondii","pmids":["25691595"],"confidence":"Medium","gaps":["No molecular activity or biochemical function assigned to IMC19","Precise assignment between alveolar membranes versus cytoskeletal network not resolved","No direct binding partners or complex membership validated for IMC19"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["inner membrane complex (IMC)"],"partners":[],"other_free_text":["Identified by BioID proximity labeling as a novel IMC component localizing to the alveolar or cytoskeletal subcompartment of the IMC."]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"25691595","id":"PMC_25691595","title":"Novel components of the Toxoplasma inner membrane complex revealed by BioID.","date":"2015","source":"mBio","url":"https://pubmed.ncbi.nlm.nih.gov/25691595","citation_count":148,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":3086,"output_tokens":339,"usd":0.007171,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4918,"output_tokens":869,"usd":0.023158,"stage2_stop_reason":"end_turn"},"total_usd":0.062724,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2015,\n      \"finding\": \"IMC19 was identified as a novel component of the Toxoplasma gondii inner membrane complex (IMC) using proximity-dependent biotin identification (BioID), localizing to a specific suborganellar compartment of the IMC (alveoli or cytoskeletal network).\",\n      \"method\": \"BioID proximity labeling in T. gondii, followed by mass spectrometry identification\",\n      \"journal\": \"mBio\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 3 / Moderate — BioID proximity labeling with MS identification in a single study; localization established but limited functional characterization specific to IMC19\",\n      \"pmids\": [\"25691595\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"IMC19 is a novel component of the Toxoplasma gondii inner membrane complex, identified by BioID proximity labeling, and resides within the IMC's alveolar or cytoskeletal subcompartments, though its specific molecular function remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"IMC19 is a component of the inner membrane complex (IMC) of Toxoplasma gondii, identified through BioID proximity labeling and localizing to a specific suborganellar subcompartment of the IMC, either its alveolar membranes or the underlying cytoskeletal network [#0]. Beyond this identification and localization, no further mechanistic detail about IMC19 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2015,\n      \"claim\": \"The composition of the Toxoplasma IMC was incompletely defined; applying BioID proximity labeling to the IMC established IMC19 as a bona fide novel constituent and placed it within a defined IMC subcompartment.\",\n      \"evidence\": \"BioID proximity labeling followed by mass spectrometry identification in T. gondii\",\n      \"pmids\": [\n        \"25691595\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No molecular activity or biochemical function assigned to IMC19\",\n        \"Precise assignment between alveolar membranes versus cytoskeletal network not resolved\",\n        \"No direct binding partners or complex membership validated for IMC19\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of IMC19, its direct interaction partners, and its role in IMC assembly or parasite biology remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional assay (knockout/knockdown) reported for IMC19\",\n        \"No structural or domain characterization\",\n        \"No defined role in motility, division, or invasion\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0070258\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"inner membrane complex (IMC)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified by BioID proximity labeling as a novel IMC component localizing to the alveolar or cytoskeletal subcompartment of the IMC.\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_217510","name":"IMC19","product":"inner membrane complex protein IMC19","uniprot_acc":"S8G8Y2","chromosome":"TGME49_chrXII","start_pos":1884821},{"strain":"GT1","gene_id":"TGGT1_217510","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrXII","start_pos":1824650},{"strain":"RH88","gene_id":"TGRH88_059410","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023093","start_pos":1896460},{"strain":"VEG","gene_id":"TGVEG_217510","name":null,"product":"hypothetical protein","uniprot_acc":"A0A125YQG1","chromosome":"TGVEG_chrXII","start_pos":1858292}]}