{"gene":"TG_212800","gene_symbol":"SPC24","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"SPC24 was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics and deep homology searches; the apicomplexan kinetochore complex that includes SPC24 is organized into at least four discrete compartments with different biochemical interactions, subkinetochore localizations, and evolutionary rates across the phylum.","method":"Interactomics (co-immunoprecipitation/proximity labeling) combined with deep homology searches and fluorescence microscopy-based subkinetochore localization","journal":"The Journal of cell biology","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — interactomics plus orthogonal localization and homology analyses in a single study; SPC24-specific data not separated from complex-level results in the abstract","pmids":["36006241"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""},{"species":"Plasmodium berghei","strain":""}]}],"current_model":"SPC24 is a newly identified, highly divergent kinetochore component in apicomplexan parasites (Toxoplasma gondii and Plasmodium berghei) that forms part of a multi-compartment kinetochore assembly involved in spindle attachment and chromosome segregation, with composition and organization showing plasticity across parasite lifecycle stages."},"narrative":{"mechanistic_narrative":"SPC24 is a highly divergent kinetochore component identified in apicomplexan parasites, forming part of a multi-compartment kinetochore assembly involved in spindle attachment and chromosome segregation [PMID:36006241]. It was recognized as one of 13 previously unknown apicomplexan kinetochore proteins, situated within a complex organized into at least four discrete compartments that differ in their biochemical interactions, subkinetochore localizations, and evolutionary rates across the phylum [PMID:36006241]. Beyond this identification and its placement within the kinetochore complex, nothing further about SPC24 is characterized in the available corpus.","teleology":[{"year":2022,"claim":"Before this work the apicomplexan kinetochore was largely undefined; identifying SPC24 as a bona fide component established that these parasites possess a divergent but organized kinetochore, resolving where SPC24 fits within chromosome segregation machinery.","evidence":"Interactomics combined with deep homology searches and fluorescence-based subkinetochore localization in Toxoplasma gondii and Plasmodium berghei","pmids":["36006241"],"confidence":"Medium","gaps":["SPC24-specific interactions and localization were not separated from complex-level results","No direct functional or knockout data establishing SPC24's individual role in spindle attachment or segregation","No structural model or defined binding partners for SPC24 itself"],"attribution":[{"species":"Toxoplasma gondii","strain":""},{"species":"Plasmodium berghei","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Apicomplexan kinetochore complex"],"partners":[],"other_free_text":["Identified as one of 13 previously unknown apicomplexan kinetochore components [#0]","Kinetochore complex containing SPC24 is organized into at least four discrete compartments with distinct biochemical interactions, subkinetochore localizations, and evolutionary rates [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"36006241","id":"PMC_36006241","title":"Composition and organization of kinetochores show plasticity in apicomplexan chromosome segregation.","date":"2022","source":"The Journal of cell biology","url":"https://pubmed.ncbi.nlm.nih.gov/36006241","citation_count":30,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2807,"output_tokens":408,"usd":0.007271,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5004,"output_tokens":1205,"usd":0.027572,"stage2_stop_reason":"end_turn"},"total_usd":0.07128,"stage1_batch_id":"msgbatch_01WokvBYTB6JLnYrpD4rXnyV","stage2_batch_id":"msgbatch_01M7hsQDvz6AqVC9DLVjQzzc","note":"batch pricing = 50% of standard","corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"SPC24 was identified as one of 13 previously unknown kinetochore components in Apicomplexa through interactomics and deep homology searches; the apicomplexan kinetochore complex that includes SPC24 is organized into at least four discrete compartments with different biochemical interactions, subkinetochore localizations, and evolutionary rates across the phylum.\",\n      \"method\": \"Interactomics (co-immunoprecipitation/proximity labeling) combined with deep homology searches and fluorescence microscopy-based subkinetochore localization\",\n      \"journal\": \"The Journal of cell biology\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — interactomics plus orthogonal localization and homology analyses in a single study; SPC24-specific data not separated from complex-level results in the abstract\",\n      \"pmids\": [\"36006241\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"SPC24 is a newly identified, highly divergent kinetochore component in apicomplexan parasites (Toxoplasma gondii and Plasmodium berghei) that forms part of a multi-compartment kinetochore assembly involved in spindle attachment and chromosome segregation, with composition and organization showing plasticity across parasite lifecycle stages.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"SPC24 is a highly divergent kinetochore component identified in apicomplexan parasites, forming part of a multi-compartment kinetochore assembly involved in spindle attachment and chromosome segregation [#0]. It was recognized as one of 13 previously unknown apicomplexan kinetochore proteins, situated within a complex organized into at least four discrete compartments that differ in their biochemical interactions, subkinetochore localizations, and evolutionary rates across the phylum [#0]. Beyond this identification and its placement within the kinetochore complex, nothing further about SPC24 is characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Before this work the apicomplexan kinetochore was largely undefined; identifying SPC24 as a bona fide component established that these parasites possess a divergent but organized kinetochore, resolving where SPC24 fits within chromosome segregation machinery.\",\n      \"evidence\": \"Interactomics combined with deep homology searches and fluorescence-based subkinetochore localization in Toxoplasma gondii and Plasmodium berghei\",\n      \"pmids\": [\n        \"36006241\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"SPC24-specific interactions and localization were not separated from complex-level results\",\n        \"No direct functional or knockout data establishing SPC24's individual role in spindle attachment or segregation\",\n        \"No structural model or defined binding partners for SPC24 itself\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"How SPC24 individually contributes to kinetochore assembly, its direct binding partners, and its functional requirement for chromosome segregation remain unresolved.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No SPC24-specific loss-of-function phenotype reported\",\n        \"Direct molecular partners of SPC24 not defined\",\n        \"No structural characterization of SPC24\"\n      ],\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"},\n        {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\"term_id\": \"GO:0000776\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}, {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]}\n    ],\n    \"pathway\": [\n      {\"term_id\": \"GO:0007059\", \"supporting_discovery_ids\": [0], \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}, {\"species\": \"Plasmodium berghei\", \"strain\": \"\"}]}\n    ],\n    \"complexes\": [\"Apicomplexan kinetochore complex\"],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified as one of 13 previously unknown apicomplexan kinetochore components [#0]\",\n      \"Kinetochore complex containing SPC24 is organized into at least four discrete compartments with distinct biochemical interactions, subkinetochore localizations, and evolutionary rates [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_212800","name":"SPC24","product":"kinetochore protein SPC24, putative","uniprot_acc":"S8FBW9","chromosome":"TGME49_chrV","start_pos":608207},{"strain":"GT1","gene_id":"TGGT1_212800","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrV","start_pos":477211},{"strain":"RH88","gene_id":"TGRH88_024160","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023086","start_pos":643357},{"strain":"VEG","gene_id":"TGVEG_212800","name":null,"product":"putative Ppg3","uniprot_acc":"B9QGL1","chromosome":"TGVEG_chrV","start_pos":573073}]}