{"gene":"TG_210255","gene_symbol":"TOM7","run_date":"2026-09-08T11:06:21","timeline":{"discoveries":[{"year":2016,"finding":"TgTom7 (T. gondii homologue of Tom7) is important for parasite survival and mitochondrial protein import, as demonstrated by loss-of-function analysis.","method":"Loss-of-function (knockdown/knockout) with readout of parasite survival and mitochondrial protein import","journal":"The Journal of biological chemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — defined loss-of-function phenotype with two readouts (survival and protein import), single lab","pmids":["27458014"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2016,"finding":"Contrary to the role of Tom7 in yeast (where it destabilizes the TOM complex), TgTom7 is important for TOM complex stability in T. gondii, indicating a diverged function during eukaryotic evolution.","method":"Loss-of-function analysis with assessment of TOM complex assembly/stability","journal":"The Journal of biological chemistry","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — direct functional comparison via loss-of-function and complex stability assay, single lab, single study","pmids":["27458014"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"TgTom7 is a component of the translocon of the outer mitochondrial membrane (TOM) complex in Toxoplasma gondii that is required for parasite survival, mitochondrial protein import, and TOM complex stability — a role distinct from its yeast ortholog, which destabilizes the TOM complex."},"narrative":{"mechanistic_narrative":"TgTom7 is a component of the translocon of the outer mitochondrial membrane (TOM) complex in Toxoplasma gondii that is required for parasite survival and mitochondrial protein import [PMID:27458014]. Loss-of-function analysis establishes that TgTom7 is important for TOM complex stability, a role that diverges from its yeast ortholog, where Tom7 destabilizes the TOM complex — indicating functional divergence during eukaryotic evolution [PMID:27458014]. Beyond these loss-of-function phenotypes, no further mechanistic detail has been characterized in the available corpus.","teleology":[{"year":2016,"claim":"Whether the Toxoplasma Tom7 homologue contributes to parasite viability and mitochondrial biogenesis was unknown; loss-of-function analysis established that TgTom7 is required for parasite survival and mitochondrial protein import, defining it as a functionally important TOM component.","evidence":"Loss-of-function (knockdown/knockout) with readouts of parasite survival and mitochondrial protein import in T. gondii","pmids":["27458014"],"confidence":"Medium","gaps":["Molecular mechanism of how TgTom7 supports protein import is not defined","Direct binding partners within the TOM complex not identified","Single lab, single study"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":2016,"claim":"Because yeast Tom7 destabilizes the TOM complex, whether the parasite ortholog retained this activity was unclear; complex-stability assays showed TgTom7 instead promotes TOM complex stability, establishing a diverged function during eukaryotic evolution.","evidence":"Loss-of-function analysis with assessment of TOM complex assembly/stability in T. gondii","pmids":["27458014"],"confidence":"Medium","gaps":["Structural basis for the stabilizing versus destabilizing role not resolved","Whether stability defect fully accounts for the import defect is untested","No reciprocal validation across independent labs"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]},{"year":null,"claim":"The molecular basis by which TgTom7 stabilizes the TOM complex and enables protein import, including its direct partners and structural arrangement, remains unresolved.","evidence":"No further mechanistic evidence in the available corpus","pmids":[],"confidence":"Medium","gaps":["No interaction map of TgTom7 within the TOM complex","No structural model","Substrate specificity of the import defect not characterized"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["TOM complex (translocon of the outer mitochondrial membrane)"],"partners":[],"other_free_text":["Required for T. gondii parasite survival","Function diverged from yeast Tom7: stabilizes rather than destabilizes the TOM complex"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"27458014","id":"PMC_27458014","title":"The Import of Proteins into the Mitochondrion of Toxoplasma gondii.","date":"2016","source":"The Journal of biological chemistry","url":"https://pubmed.ncbi.nlm.nih.gov/27458014","citation_count":42,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2871,"output_tokens":499,"usd":0.008049,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":5174,"output_tokens":1529,"usd":0.032047,"stage2_stop_reason":"end_turn"},"total_usd":0.040096,"stage1_batch_id":"msgbatch_01WokvBYTB6JLnYrpD4rXnyV","stage2_batch_id":"msgbatch_01M7hsQDvz6AqVC9DLVjQzzc","note":"batch pricing = 50% of standard"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2016,\n      \"finding\": \"TgTom7 (T. gondii homologue of Tom7) is important for parasite survival and mitochondrial protein import, as demonstrated by loss-of-function analysis.\",\n      \"method\": \"Loss-of-function (knockdown/knockout) with readout of parasite survival and mitochondrial protein import\",\n      \"journal\": \"The Journal of biological chemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — defined loss-of-function phenotype with two readouts (survival and protein import), single lab\",\n      \"pmids\": [\"27458014\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    },\n    {\n      \"year\": 2016,\n      \"finding\": \"Contrary to the role of Tom7 in yeast (where it destabilizes the TOM complex), TgTom7 is important for TOM complex stability in T. gondii, indicating a diverged function during eukaryotic evolution.\",\n      \"method\": \"Loss-of-function analysis with assessment of TOM complex assembly/stability\",\n      \"journal\": \"The Journal of biological chemistry\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — direct functional comparison via loss-of-function and complex stability assay, single lab, single study\",\n      \"pmids\": [\"27458014\"],\n      \"is_preprint\": false,\n      \"attribution\": [{\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}]\n    }\n  ],\n  \"current_model\": \"TgTom7 is a component of the translocon of the outer mitochondrial membrane (TOM) complex in Toxoplasma gondii that is required for parasite survival, mitochondrial protein import, and TOM complex stability — a role distinct from its yeast ortholog, which destabilizes the TOM complex.\"\n}\n```","stage2_raw":"```json\n{\n  \"mechanistic_narrative\": \"TgTom7 is a component of the translocon of the outer mitochondrial membrane (TOM) complex in Toxoplasma gondii that is required for parasite survival and mitochondrial protein import [#0]. Loss-of-function analysis establishes that TgTom7 is important for TOM complex stability, a role that diverges from its yeast ortholog, where Tom7 destabilizes the TOM complex \\u2014 indicating functional divergence during eukaryotic evolution [#1]. Beyond these loss-of-function phenotypes, no further mechanistic detail has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2016,\n      \"claim\": \"Whether the Toxoplasma Tom7 homologue contributes to parasite viability and mitochondrial biogenesis was unknown; loss-of-function analysis established that TgTom7 is required for parasite survival and mitochondrial protein import, defining it as a functionally important TOM component.\",\n      \"evidence\": \"Loss-of-function (knockdown/knockout) with readouts of parasite survival and mitochondrial protein import in T. gondii\",\n      \"pmids\": [\n        \"27458014\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Molecular mechanism of how TgTom7 supports protein import is not defined\",\n        \"Direct binding partners within the TOM complex not identified\",\n        \"Single lab, single study\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": 2016,\n      \"claim\": \"Because yeast Tom7 destabilizes the TOM complex, whether the parasite ortholog retained this activity was unclear; complex-stability assays showed TgTom7 instead promotes TOM complex stability, establishing a diverged function during eukaryotic evolution.\",\n      \"evidence\": \"Loss-of-function analysis with assessment of TOM complex assembly/stability in T. gondii\",\n      \"pmids\": [\n        \"27458014\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"Structural basis for the stabilizing versus destabilizing role not resolved\",\n        \"Whether stability defect fully accounts for the import defect is untested\",\n        \"No reciprocal validation across independent labs\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The molecular basis by which TgTom7 stabilizes the TOM complex and enables protein import, including its direct partners and structural arrangement, remains unresolved.\",\n      \"evidence\": \"No further mechanistic evidence in the available corpus\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No interaction map of TgTom7 within the TOM complex\",\n        \"No structural model\",\n        \"Substrate specificity of the import defect not characterized\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [\n      {\n        \"term_id\": \"GO:0015450\",\n        \"supporting_discovery_ids\": [\n          0\n        ],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"localization\": [\n      {\n        \"term_id\": \"GO:0005741\",\n        \"supporting_discovery_ids\": [\n          0,\n          1\n        ],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [\n      {\n        \"term_id\": \"GO:0030150\",\n        \"supporting_discovery_ids\": [\n          0\n        ],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"complexes\": [\n      \"TOM complex (translocon of the outer mitochondrial membrane)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Required for T. gondii parasite survival\",\n      \"Function diverged from yeast Tom7: stabilizes rather than destabilizes the TOM complex\"\n    ]\n  },\n  \"strain_conflicts\": []\n}\n```","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_210255","name":"TOM7","product":"mitochondrial import receptor subunit TOM7","uniprot_acc":"S8G8T8","chromosome":"TGME49_chrIX","start_pos":4925289},{"strain":"GT1","gene_id":"TGGT1_210255","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrIX","start_pos":4719308},{"strain":"RH88","gene_id":"TGRH88_016510","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023090","start_pos":4996012},{"strain":"VEG","gene_id":"TGVEG_210255","name":null,"product":"putative transmembrane protein","uniprot_acc":"A0A0F7V0X3","chromosome":"TGVEG_chrIX","start_pos":4969234}]}