{"gene":"TG_200330","gene_symbol":"BCC9","run_date":"2026-09-10T21:09:19","timeline":{"discoveries":[{"year":2022,"finding":"BCC9 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach, placing it within one of four architecturally distinct BC subcomplexes whose compositions change dynamically in correlation with changes in BC function during cell division.","method":"Reciprocal proximity-based biotinylation (BioID) proteomics with spatiotemporal and functional interrogation by disrupting expression of components","journal":"Nature communications","confidence":"Medium","confidence_rationale":"Tier 2 / Moderate — reciprocal BioID proteomics with functional disruption experiments in a single study; BCC9 is listed as a BC component but no specific mechanistic detail beyond complex membership is reported for BCC9 individually","pmids":["35941170"],"is_preprint":false,"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"current_model":"BCC9 is a basal complex component of Toxoplasma gondii, identified by proximity-based proteomics as part of one of four architecturally distinct subcomplexes whose composition changes dynamically during cell division, but its specific molecular function within the basal complex remains uncharacterized."},"narrative":{"mechanistic_narrative":"BCC9 is a component of the Toxoplasma gondii basal complex, identified through reciprocal proximity-based biotinylation (BioID) proteomics as part of one of four architecturally distinct basal complex subcomplexes whose compositions change dynamically in correlation with basal complex function during cell division [PMID:35941170]. Beyond its assignment as a basal complex component [PMID:35941170], no further mechanistic detail about BCC9 has been characterized in the available corpus.","teleology":[{"year":2022,"claim":"Establishing which proteins populate the Toxoplasma basal complex was needed to understand its architecture; BCC9 was placed within one of four distinct basal complex subcomplexes, defining it as a bona fide basal complex component.","evidence":"Reciprocal proximity-based biotinylation (BioID) proteomics with spatiotemporal and functional interrogation in Toxoplasma gondii","pmids":["35941170"],"confidence":"Medium","gaps":["No specific molecular function for BCC9 within the basal complex was determined","Direct binding partners and substrate/interaction interfaces of BCC9 are not resolved","The consequence of BCC9 loss on basal complex assembly or cell division is not established"],"attribution":[{"species":"Toxoplasma gondii","strain":""}]}],"mechanism_profile":{"molecular_activity":[],"localization":[],"pathway":[],"complexes":["Toxoplasma gondii basal complex (one of four architecturally distinct subcomplexes)"],"partners":[],"other_free_text":["Identified by reciprocal proximity-based biotinylation (BioID) proteomics as a basal complex component whose subcomplex composition changes dynamically during cell division [#0]"]},"strain_conflicts":[]},"prefetch_data":{},"corpus_meta":[{"pmid":"35941170","id":"PMC_35941170","title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function.","date":"2022","source":"Nature communications","url":"https://pubmed.ncbi.nlm.nih.gov/35941170","citation_count":46,"is_preprint":false}],"cost":{"stage1":{"model":"claude-sonnet-4-6","input_tokens":2793,"output_tokens":359,"usd":0.006882,"stage1_stop_reason":"end_turn"},"stage2":{"model":"claude-opus-4-8","input_tokens":4949,"output_tokens":1054,"usd":0.025548,"stage2_stop_reason":"end_turn"},"total_usd":0.033303,"stage1_batch_id":"msgbatch_01WgHz6kceqm573AGLyz57at","stage2_batch_id":"msgbatch_01LK4wDYj5zYquahv7gi9893","note":"batch pricing = 50% of standard","skipped":true,"corrective_rerun":"2026-09-10 wrong-gene rerun (round-4 rules)","corrective_reason":"re-read with subject anchor"},"stage1_raw":"```json\n{\n  \"discoveries\": [\n    {\n      \"year\": 2022,\n      \"finding\": \"BCC9 was identified as a component of the Toxoplasma gondii basal complex (BC) proteome through a reciprocal proximity-based biotinylation (BioID) approach, placing it within one of four architecturally distinct BC subcomplexes whose compositions change dynamically in correlation with changes in BC function during cell division.\",\n      \"method\": \"Reciprocal proximity-based biotinylation (BioID) proteomics with spatiotemporal and functional interrogation by disrupting expression of components\",\n      \"journal\": \"Nature communications\",\n      \"confidence\": \"Medium\",\n      \"confidence_rationale\": \"Tier 2 / Moderate — reciprocal BioID proteomics with functional disruption experiments in a single study; BCC9 is listed as a BC component but no specific mechanistic detail beyond complex membership is reported for BCC9 individually\",\n      \"pmids\": [\"35941170\"],\n      \"is_preprint\": false,\n      \"attribution\": [\n        {\"species\": \"Toxoplasma gondii\", \"strain\": \"\"}\n      ]\n    }\n  ],\n  \"current_model\": \"BCC9 is a basal complex component of Toxoplasma gondii, identified by proximity-based proteomics as part of one of four architecturally distinct subcomplexes whose composition changes dynamically during cell division, but its specific molecular function within the basal complex remains uncharacterized.\"\n}\n```","stage2_raw":"{\n  \"mechanistic_narrative\": \"BCC9 is a component of the Toxoplasma gondii basal complex, identified through reciprocal proximity-based biotinylation (BioID) proteomics as part of one of four architecturally distinct basal complex subcomplexes whose compositions change dynamically in correlation with basal complex function during cell division [#0]. Beyond its assignment as a basal complex component [#0], no further mechanistic detail about BCC9 has been characterized in the available corpus.\",\n  \"teleology\": [\n    {\n      \"year\": 2022,\n      \"claim\": \"Establishing which proteins populate the Toxoplasma basal complex was needed to understand its architecture; BCC9 was placed within one of four distinct basal complex subcomplexes, defining it as a bona fide basal complex component.\",\n      \"evidence\": \"Reciprocal proximity-based biotinylation (BioID) proteomics with spatiotemporal and functional interrogation in Toxoplasma gondii\",\n      \"pmids\": [\n        \"35941170\"\n      ],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No specific molecular function for BCC9 within the basal complex was determined\",\n        \"Direct binding partners and substrate/interaction interfaces of BCC9 are not resolved\",\n        \"The consequence of BCC9 loss on basal complex assembly or cell division is not established\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    },\n    {\n      \"year\": null,\n      \"claim\": \"The specific molecular function of BCC9 within the basal complex, its direct partners, and its role in cell division remain unknown.\",\n      \"evidence\": \"\",\n      \"pmids\": [],\n      \"confidence\": \"Medium\",\n      \"gaps\": [\n        \"No functional assay isolates BCC9's individual contribution to basal complex function\",\n        \"No structural or interaction model for BCC9 exists in the corpus\"\n      ],\n      \"attribution\": [\n        {\n          \"species\": \"Toxoplasma gondii\",\n          \"strain\": \"\"\n        }\n      ]\n    }\n  ],\n  \"mechanism_profile\": {\n    \"molecular_activity\": [],\n    \"localization\": [\n      {\n        \"term_id\": \"basal complex\",\n        \"supporting_discovery_ids\": [0],\n        \"attribution\": [\n          {\n            \"species\": \"Toxoplasma gondii\",\n            \"strain\": \"\"\n          }\n        ]\n      }\n    ],\n    \"pathway\": [],\n    \"complexes\": [\n      \"Toxoplasma gondii basal complex (one of four architecturally distinct subcomplexes)\"\n    ],\n    \"partners\": [],\n    \"other_free_text\": [\n      \"Identified by reciprocal proximity-based biotinylation (BioID) proteomics as a basal complex component whose subcomplex composition changes dynamically during cell division [#0]\"\n    ]\n  },\n  \"strain_conflicts\": []\n}","audit_flag":null,"strains":[{"strain":"ME49","gene_id":"TGME49_200330","name":"BCC9","product":"basal complex component BCC9","uniprot_acc":"A0A125YJ55","chromosome":"TGME49_chrVIII","start_pos":6803584},{"strain":"GT1","gene_id":"TGGT1_200330","name":null,"product":"hypothetical protein","uniprot_acc":null,"chromosome":"TGGT1_chrVIII","start_pos":6780776},{"strain":"RH88","gene_id":"TGRH88_083170","name":null,"product":"unspecified product","uniprot_acc":null,"chromosome":"CM023089","start_pos":11932227},{"strain":"VEG","gene_id":"TGVEG_200330","name":null,"product":"hypothetical protein","uniprot_acc":"B9Q3N5","chromosome":"TGVEG_chrVIII","start_pos":6831432}]}